井长勤;刘振华;李文锦;张尊;王林林;

• 1:河南师范大学生命科学学院
• 2:新乡医学院生命科学技术学院

摘要(Abstract)：

为探讨咪唑类离子液体氯化1-辛基-3-甲基咪唑([C8mim][Cl])对酵母细胞的增殖生长和细胞膜通透性的影响,以不同浓度的[C8mim][Cl]处理酵母细胞,研究离子液体对酵母细胞增殖和菌落形成的影响,通过测定酵母细胞外液蛋白质和核酸的含量,判断膜通透性的大小和[C8mim][Cl]对细胞膜性结构的损伤。结果显示,0.1 mmol·L-1的[C8mim][Cl]延长了酵母细胞到达对数生长期的时间,在6~9 h之间对酵母细胞的增殖存在明显的抑制作用。1 mmol·L-1的[C8mim][Cl]使酵母细胞增殖不能到达对数生长期,对酵母细胞的增殖一直具有较强的抑制作用。随着离子液体浓度的增加,小菌落的数量增多。当平板内[C8mim][Cl]浓度达到10 mmol·L-1时,完全抑制了菌落的形成。[C8mim][Cl]处理酵母细胞后,细胞外液中OD280、OD260的值显著升高。研究表明,细胞膜等膜性结构通透性的增加是离子液体[C8mim][Cl]抑制酵母细胞增殖生长的原因之一。

关键词(KeyWords)： 离子液体;酿酒酵母;增殖生长;膜通透性

Abstract:

Keywords:

基金项目(Foundation): 河南省科技攻关重点项目(12A180022)

作者(Author): 井长勤;刘振华;李文锦;张尊;王林林;

Email:

参考文献(References)：

• [1]Sheldon R.Catalytic reactions in ionic liquids[J].Chemical Communications(Cambridge),2001(23):2399-2407
• [2]Li X Y,Jing C Q,Zang X Y,et al.Toxic cytological alteration and mitochondrial dysfunction in PC12 cells induced by 1-octyl-3-methylimidazolium chloride[J].Toxicology in Vitro,2012,26(7):1087-1092
• [3]张保芳,蒲敏,陈标华,等.一类新型绿色环保的介质材料——咪唑类离子液体[J].材料科学与工程学报,2006,24(1):165-168Zhang B F,Pu M,Chen B H,et al.New environmentalfriend material—imidazolium-based ionic liquids[J].Journal of Materials Science and Engineering,2006,24(1):165-168(in Chinese)
• [4]柯明,周爱国,宋昭峥,等.离子液体的毒性[J].化学进展,2007,19(5):671-679Ke M,Zhou A G,Song Z Z,et al.Toxicity of ionic liquids[J].Progress in Chemistry,2007,19(5):671-679(in Chinese)
• [5]徐红梅,田芳,靳丹萍,等.咪唑型离子液体对小鼠的急性毒性[J].环境科学学报,2011,31(2):420-423Xu H M,Tian F,Jin D P,et al.Acute toxicity of imidazolium ionic liquid to mice[J].Acta Scientiae Circumstantiae,2011,31(2):420-423(in Chinese)
• [6]Dong M,Zhu L,Zhu S,et al.Toxic effects of 1-decyl-3-methylimidazolium bromide ionic liquid on the antioxidant enzyme system and DNA in zebrafish(Danio rerio)livers[J].Chemosphere,2013,91(8):1107-1112
• [7]Li X Y,Zeng S H,Dong X Y,et al.Acute toxicity and responses of antioxidant systems to 1-methyl-3-octylimidazolium bromide at different developmental stages of goldfish[J].Ecotoxicology,2012,21(1):253-259
• [8]李效宇,曾师虎,张伟红,等.离子液体溴化1-辛基-3-甲基咪唑对太行隆肛蛙蝌蚪的毒性及其抗氧化系统的影响[J].河南师范大学学报:自然科学版,2010,38(1):138-141Li X Y,Zeng S H,Zhang W H,et al.Acute and subacute toxicity of 1-octyl-3-methylimidazolium bromide on Feirana taihangnicus tadpoles and effects on the antioxidant enzymes and lipid peroxidation of frog tadpoles[J].Journal of Henan Normal University:Natural Science,2010,38(1):138-141(in Chinese)
• [9]洪霞,薛永来,戴志聪,等.1-丁基-3-甲基咪唑三氟乙酸盐对小麦种子萌发和幼苗生长的毒性[J].江苏农业科学,2012,40(8):74-77Hong X,Xue Y L,Dai Z C,et al.Effects of[Bmim]CF3COOH on seed germination and seedlings growth in wheat[J].Jiangsu Agricultural Sciences,2012,40(8):74-77(in Chinese)
• [10]杨芬芬,孟洪,李春喜,等.离子液体对三种农作物发芽和生长的毒性研究[J].环境工程学报,2009,3(4):751-754Yang F F,Meng H,Li C X,et al.Ecotoxicity of ionic liquids on the germination and growth of three seeds[J].Chinese Journal of Environmental Engineering,2009,3(4):751-754(in Chinese)
• [11]Samori C,Malferrari D,Valbonesi P,et al.Introduction of oxygenated side chain into imidazolium ionic liquids:Evaluation of the effects at different biological organization levels[J].Ecotoxicology and Environmental Safety,2010,73(6):1456-1464
• [12]Kumar M,Trivedi N,Reddy C R,et al.Toxic effects of imidazolium ionic liquids on the green seaweed Ulva lactuca:Oxidative stress and DNA damage[J].Chemical Research in Toxicology,2011,24(11):1882-1890
• [13]Li X Y,Dong X Y,Bai X,et al.The embryonic and postembryonic developmental toxicity of imidazoliumbased ionic liquids on Physa acuta[J].Environmental Toxicology,2012,Jul 30.doi:10.1002/tox.21797.
• [14]Li X Y,Luo Y R,Yun M X,et al.Effects of 1-methyl-3-octylimidazolium bromide on the anti-oxidant system of earthworm[J].Chemosphere,2010,78(7):853-858
• [15]Swatloski R P,Holbrey J D,Memon S B,et al.Using Caenorhabditis elegans to probe toxicity of 1-alkyl-3-methylimidazolium chloride based ionic liquids[J].Chemical Communications(Cambridge),2004(6):668-669
• [16]Ventura S P,Marques C S,Rosatella A A,et al.Toxicity assessment of various ionic liquid families towards Vibrio fischeri marine bacteria[J].Ecotoxicology and Environmental Safety,2012,76(2):162-168
• [17]Jing C Q,liu Y T,Shen X Y,et al.Toxic evaluation of the alkylmethylimidazolium-based ionic liquids in yeast Saccharomyces cerevisiae by flow cytometry[J].Toxin Reviews,2012,31(1-4):27-31
• [18]刘芳,刘树深,刘海玲.部分离子液体及其混合物对发光菌的毒性作用[J].生态毒理学报,2007,2(2):164-171Liu F,Liu S S,Liu H L.Toxicities of selected ionic liquids and their mixtures to photobacteria[J].Asian Journal of Ecotoxicology,2007,2(2):164-171(in Chinese)
• [19]Stock F,Hoffmann J,Ranke J,et al.Effects of ionic liquids on the acetylcholinesterase-A structure-activity relationship consideration[J].Green Chemistry,2004(6):286-290
• [20]Rusiecka I,Skladanowski A C.Induction of the multixenobiotic/multidrug resistance system in HeLa cells in response to imidazolium ionic liquids[J].Acta Biochimica Polonica,2011,58(2):187-192
• [21]Li X Y,Jing C Q,Lei W L,et al.Apoptosis caused by imidazolium-based ionic liquids in PC12 cells[J].Ecotoxicology and Environmental Safety,2012,83:102-107
• [22]Kashiwagi A,Urabe I,Kaneko K,et al.Adaptive response of a gene network to environmental changes by fitness-induced attractor selection[J].PLoS One,2006,1:e49
• [23]李习艺,庄志雄,刘建军,等.低剂量氢醌、甲醛及过氧化氢刺激人胚肺成纤维细胞适应性反应的共同差异蛋白的表达[J].中国药理学与毒理学杂志,2007,21(5):410-415Li X Y,Zhuang Z X,Liu J J,et al.Expression of proteins for adaptive response induced by low concentrations of hyderoquinone,formaldehyde and hydrogen peroxide in human embryonic lung fibroblasts[J].Chinese Journal of Pharmacology and Toxicology,2007,21(5):410-415(in Chinese)
• [24]管增伟,王盛兰,李勇,等.凋亡细胞细胞膜和线粒体的动态变化[J].卫生研究,2000,29(2):83-86Guan Z W,Wang S L,Li Y,et al.Kinetics of plasma membrane and mitochondrial alterations in HL-60 cells undergoing apoptosis[J].Journal of Hygiene Research,2000,29(2):83-86(in Chinese)