肖琳;付聪;梁思斯;毛彩霞;袭著革;杨旭;

• 1:华中师范大学生命科学学院
• 2:华中师范大学生命科学学院
• 3:华中师范大学生命科学学院
• 4:华中师范大学生命科学学院

摘要(Abstract)：

为了探讨纳米SiO2和常规SiO2颗粒对Hela细胞的细胞毒性作用,采用不同浓度的纳米SiO2和常规SiO2颗粒(0.05、0.1、0.2、0.4、0.8、1.6μg·μL-1)对Hela细胞进行12h染毒,应用MTT法检测细胞毒性效应.研究发现,较低浓度(≤0.2μg·μL-1)的纳米SiO2和常规SiO2对Hela细胞无明显细胞毒性(p>0.05);较高浓度时,纳米SiO2(≥0.4μg·μL-1)和常规SiO2(≥0.8μg·μL-1)对Hela细胞具有明显细胞毒性作用(p<0.01),并且随浓度增大细胞毒性增强;当浓度≥0.4μg·μL-1时,纳米SiO2的细胞毒性明显高于相同浓度的常规SiO2(p<0.05).以上结果表明,纳米SiO2和常规SiO2颗粒均能对Hela细胞产生细胞毒性,且纳米SiO2的细胞毒性强于常规SiO2;低浓度(≤0.2μg·μL-1)的纳米SiO2和常规SiO2具有很好的生物相容性.

关键词(KeyWords)： 纳米SiO2;常规SiO2;Hela细胞;细胞毒性;MTT法

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展规划(863)项目(No.2006AA03Z330)

作者(Author): 肖琳;付聪;梁思斯;毛彩霞;袭著革;杨旭;

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