葛会林;刘树深;朱祥伟;王丽娟;

• 1:同济大学环境科学与工程学院长江水环境教育部重点实验室
• 2:西南大学生命科学学院三峡库区生态环境教育部重点实验室

摘要(Abstract)：

以斜生栅藻(Scenedesmus obliquus)为指示生物,96微孔板为暴露反应载体,SpectraMax M5酶标仪为吸光度测试设备,建立了测定毒物对藻生长抑制毒性的微板吸光法.论文系统研究了微板吸光法中斜生栅藻的可见吸收光谱和生长曲线以及pH和暴露时间对藻生长的影响,同时应用该方法成功测定了环嗪酮、阿特拉津、西草净、扑灭通、苯嗪草酮、敌草快、草甘膦7种除草剂和磷胺、甲胺磷2种杀虫剂对斜生栅藻的剂量-效应曲线(DRC).通过对剂量-效应数据进行非线性最小二乘模拟,获得了这些农药的半数效应浓度EC50及置信区间.对比标准锥形瓶栅藻毒性试验,微板吸光法具有测试简便快速,所需样品体积少,便于多次平行毒性测试等优点.

关键词(KeyWords)： 斜生栅藻;除草剂;杀虫剂;微板藻毒性;剂量-效应曲线

Abstract:

Keywords:

基金项目(Foundation): 国家863计划专题课题(No.2007AA06Z417);; 国家自然科学基金项目(No.20777056)

作者(Author): 葛会林;刘树深;朱祥伟;王丽娟;

Email:

DOI:

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