低浓度五氯酚暴露对稀有鮈鲫体内SOD活性、GSH和HSP70含量的影响Effects of Low Concentration of Pentachlorophenol Exposure on SOD Activity,GSH and HSP70 Content in Rare Minnow(Gobiocypris rarus)
王辅明;朱祥伟;马永鹏;刘树深;刘堰;
摘要(Abstract):
为筛选检测低浓度五氯酚(PCP)对水生生物毒性效应较敏感的指标,将稀有鮈鲫(Gobiocypris rarus)暴露于浓度分别为0.5、1.5、5.0、15和50μg·L-1的PCP中2、7、14、21d,同时设乙醇对照组,检测稀有鮈鲫体内(整体匀浆)超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)、热激蛋白70(HSP70)含量等生化指标.结果表明,相同暴露时间下,随着PCP暴露浓度的升高,SOD活性均表现为先抑制后激活趋势,GSH和HSP70含量无明显变化;相同暴露浓度下,随着PCP暴露时间的延长,SOD活性均表现为先激活后抑制趋势,GSH和HSP70含量无明显变化.初步认定相对于GSH、HSP70含量,SOD活性的变化可以更有效地反映低浓度PCP对稀有鮈鲫的毒性效应.
关键词(KeyWords): 稀有鮈鲫;PCP;SOD;GSH;HSP70
基金项目(Foundation): 三峡库区生态环境教育部重点实验室科学研究基金;; 全国优秀博士学位论文作者基金(No.200355)
作者(Author): 王辅明;朱祥伟;马永鹏;刘树深;刘堰;
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参考文献(References):
- Cao J H,Ma G Z,Fang Z Q.2004.Effects of cadmiumexposure on SOD activity in gill and liver of grass carp[J].Reservoir Fisheries,24(1):9-10(in Chinese)
- Cao W X,Wang J W.2003.Rare minnoe:A new laboratoryanimal in China[J].Laboratory Animal Science and Management,20(1):96-99(in Chinese)
- Chen H G,Li Z L,Xu Y,Kong Z M,Liu Z T.2006.DNAdamage induced by sodium pentachlorophenate in crucian kidneycells in vivo and in vitro[J].Journal of Environment andHealth,23(6):515-517(in Chinese)
- Fang Z,Liu Z T,Wang W H,Li J,Li Z,Zheng M H.2006.Influence of lymphocyte from human peripheral blood by sodiumpentachlorophenate and dioxons remnant toxicant[J].ChinaEnvironmental Science,26(1):39-42(in Chinese)
- Feder M E,Hofmann G E.1999.Heat-shock proteins,molecularchaperones,and the stress response:evolutionary and ecologicalphysiology[J].Annual Review of Physiology,61:243-282
- Forreiter C,Nover L.1998.Heat induced stress proteins and theconcept of molecular chaperones[J].Journal of Biosciences,23(4):287-302
- Glover J R,Lindquist S.1998.HSP104,HSP70,and HSP40:anovel chaperone system that rescues previously aggregated proteins[J].Cell,94(1):73-82
- Hightower L E.1991.Heat shock,stress proteins,chaperones,and proteotoxicity[J].Cell,66(2):191-197
- Iwama G K,Thomas P T,Forsyth R B,Vijayan M M.1998.Heat shock protein expression in fish[J].Reviews in FishBiology and Fisheries,8(1):35-56
- JttelM.1999.Escaping cell death:survival proteins in cancer[J].Experimental Cell Research,248(1):30-43
- Jiang L,Zhou Z,Hu J,Wu Y D,Zhang J,Liang Y,Zheng MH.2008.Combined toxicity of pentachlorophenol(PCP)andoctachlorodibenzo-p-dioxin(OCDD)on development of zebrafish(Danio rerio)embryos[J].Asian Journal of Ecotoxicology,3(4):370-376(in Chinese)
- Li W M,Yin D Q,Zhou Y,Zhang M,Wang L S.2003.Pentachlorophenol-induced oxidative stress in liver of crucian carp[J].Rural Eco-Environment,19(1):40-42(in Chinese)
- Liu H,Wang X R,Wang W M,Shen H.2005.Effects oflong-term exposure of low level zinc and Zn-EDTA complexionzinc accumulation and antioxidant defense system in liver ofCarassius auratus[J].Environmental Science,26(1):173-176(in Chinese)
- Ma X Y,Fan L M,Gan J L,Hu G D,Qu J H,Chen J Z.2007.Effects of TBT on the activities of superoxide dismutaseand the content of glutathione in two tissues of Oreochromisaureus[J].Journal of Safety and Environment,7(4):9-11(inChinese)
- MenpK A,Penttinen O,Kukkonen J V K.2004.Pentachlorophenol(PCP)bioaccumulation and effect on heatproduction on salmon eggs at different stages of development[J].Aquatic Toxicology,68(1):75-85
- Meng S L,Chen J Z,Hu G D,Qu J H,Leng C M.2007.Effects of low level atrazine exposure on the activity ofsuperoxide dismutase(SOD)in Carassius auratus[J].Journal ofAgro-Environment Science,26(1):170-174(in Chinese)
- Mittler R.2002.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Science,7(9):405-410
- Nover L.1991.Heat Shock Response[M].Boca Raton:CRCPress,1-487
- Pavlica M,Klobucar G I,Mojas N,Erben R,Papes D.2001.Detection of DNA damage in haemocytes of zebra mussel usingcomet assay[J].Mutation Research,490(2):209-214
- Pavlica M,Klobucar G I,Vetma N,Erben R,Papes D.2000.Detection of micronuclei in haemocytes of zebra mussel and greatramshorn snail exposed to pentachlorophenol[J].MutationResearch,465(1-2):145-150
- Song R X,Ruan H J,Jing X Y,Liu Z T.2007.Genotoxicityof Sodium Pentachlorophenol[J].Carcinogenesis,Teratogenesis&Mutagenesis,19(5):355-357(in Chinese)
- Wang F,Chen Y Y,Lin S B.1997.Chemical assay of chlorophenolin water and fish guts[J].Foreign Medical Sciences(SectionHygiene),24(3):162-164(in Chinese)
- Wang J H,Liu H X,Xu T.1989.The role of superoxidedismutase(SOD)in stress physiology and senescence physiologyof plant[J].Plant Physiology Communications,1(1):1-7(inChinese)
- Xu L H,Zhang Y Y,Chen Y Y.1995.The advances of molecularecotoxicology and its significance in water environment protection[J].Acta Hydrobiologica Sinica,19(2):171-185(in Chinese)
- Yang S Z,Han X D,Chen W.2005.Advances in the toxicityof pentachlorophenol on organism[J].Journal of Environmentand Health,22(5):396-398(in Chinese)
- Zhang M,Gu Y F,Gu Y,Wang B,Yin D Q.2005.Cytotoxicitytesting of pentachlorophenol in lower concentration to blood cellsof Cyprinus Carpios in vitro[J].Environmental Chemistry,24(3):302-305(in Chinese)
- Zhang W S,Xu X Q,Zhou J Z,Fan W Z,Zhang B S,ZhouJ A,Yang B F.1995.Environmental pollution and effect ofcrowd health of pentachlorophenol in Jintan region[J].JiangsuJournal of Preventive Medicine,(2):20-23(in Chinese)
- Zheng M,Zhu L.2005.Toxicity effects of pentachlorophenol onBrachydanio rerio[J].Chinese Journal of Applied Ecology,16(10):1967-1971(in Chinese)
- Zhou J,Li Y B.2007.Algae Hepatotoxicity and superoxidedismutase(SOD)activity induced by mixture of nickel sulfate,potassium dichromate and cobalt chloride[J].Journal of MedicalResearch,36(1):89-90(in Chinese)
- Zhou Q F,Jiang G B,Liu J Y.2003.Studies on the acute anchronic toxicity of Tributyl Tin compound on Rare Minnow[J]Science in China(Series B),33(2):150-156(in Chinese)
- Zhou Y X,Chen S P,Hu W,Sun M J.1995.A new toxicitytest organism—Gobiocypris rarus[J].Zoological Research,16(1):59-63(in Chinese)
- Zhou Y X,Chen S P,Sun M J,Hu W.1994.Acute,subchronicand chronic toxicity of pentanchlorophenol to Daphnia magna[J].Zoological Research,15(4):79-84(in Chinese)
- 曹剑辉,马广智,方展强.2004.镉对草鱼鳃和肝组织超氧化物歧化酶活性的影响[J].水利渔业,24(1):9-10
- 曹文宣,王剑伟.2003.稀有鮈鲫——一种新的鱼类实验动物[J].实验动物科学与管理,20(S1):96-99
- 陈海刚,李兆利,徐韵,孔志明,刘征涛.2006.五氯酚钠对鲤鱼肾细胞DNA损伤的体内和体外研究[J].环境与健康,23(6):515-517
- 方征,刘征涛,王婉华,李霁,李政,郑明辉.2006.五氯酚钠及二噁英残留毒物对人外周血淋巴细胞的影响[J].中国环境科学,26(1):39-42
- 蒋琳,周珍,胡俊,吴艳娣,张捷,梁勇,郑明辉.2008.五氯酚和八氯代二苯并二噁英复合物暴露对斑马鱼胚胎发育的毒性效应[J].生态毒理学报,3(4):370-376
- 李伟民,尹大强,周岩,张民,王连生.2003.五氯酚对鲫鱼肝脏的氧化损伤[J].农村生态环境,19(1):40-42
- 刘慧,王晓蓉,王为木,沈骅.2005.低浓度锌及其EDTA配合物长期暴露对鲫鱼肝脏锌富集及抗氧化系统的影响[J].环境科学,26(1):173-176
- 马晓燕,范立民,甘居利,胡庚东,瞿建宏,陈家长.2007.三丁基锡(TBT)对罗非鱼两组织SOD和GSH的影响[J].安全与环境学报,7(4):9-11
- 孟顺龙,陈家长,胡庚东,瞿建宏,冷春梅.2007.低浓度阿特拉津对鲫鱼超氧化物歧化酶(SOD)活性的影响[J].农业环境科学学报,26(1):170-174
- 宋瑞霞,阮鸿洁,景欣月,刘征涛.2007.五氯酚钠的细胞遗传毒性研究[J].癌变·畸变·突变,19(5):355-357
- 王芳,陈亚妍,林少彬.1997.水及鱼胆中氯酚的化学测定[J].国外医学(卫生学分册),24(3):162-164
- 王建华,刘鸿先,徐同.1989.超氧化物歧化酶(SOD)在植物逆境和衰老中的作用[J].植物生理学通讯,1(1):1-7
- 徐立红,张甬元,陈宜瑜.1995.分子生态毒理学研究进展及其在水环境保护中的意义[J].水生生物学报,19(2):171-185
- 杨淑贞,韩晓冬,陈伟.2005.五氯酚对生物体的毒性研究进展[J].环境与健康杂志,22(5):396-398
- 张民,顾宇飞,顾颖,王斌,尹大强.2005.低浓度五氯酚对鲫鱼血液细胞毒性的体外研究[J].环境化学,24(3):302-305
- 张文生,许小青,周静珍,樊文中,张培生,周金敖,杨炳富.1995.金坛地区五氯酚环境污染及其对人群健康影响[J].江苏预防医学,2:20-23
- 郑敏,朱琳.2005.五氯酚对斑马鱼胚胎的毒性效应研究[J].应用生态学报,16(10):1967-1971
- 周继,李玉白.2007.鹅膏蕈及镍铬钴混合物对小鼠肝毒性及SOD活性的影响[J].医学研究杂志,36(1):89-90
- 周群芳,江桂斌,刘稷燕.2003.三丁基锡化合物对稀有鮈鲫的急慢性毒理研究[J].中国科学(B辑),33(2):150-156
- 周永欣,成水平,胡炜,孙美娟.1995.稀有鮈鲫——一种新的鱼类毒性试验材料[J].动物学研究,16(1):59-63
- 周永欣,成水平,孙美娟,胡炜.1994.五氯酚对大型溞的急性亚慢性和慢性毒性[J].动物学研究,15(4):79-84