低浓度五氯酚暴露对稀有鮈鲫体内SOD活性、GSH和HSP70含量的影响Effects of Low Concentration of Pentachlorophenol Exposure on SOD Activity,GSH and HSP70 Content in Rare Minnow(Gobiocypris rarus)
王辅明;朱祥伟;马永鹏;刘树深;刘堰;
摘要(Abstract)：
为筛选检测低浓度五氯酚(PCP)对水生生物毒性效应较敏感的指标,将稀有鮈鲫(Gobiocypris rarus)暴露于浓度分别为0.5、1.5、5.0、15和50μg·L-1的PCP中2、7、14、21d,同时设乙醇对照组,检测稀有鮈鲫体内(整体匀浆)超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)、热激蛋白70(HSP70)含量等生化指标.结果表明,相同暴露时间下,随着PCP暴露浓度的升高,SOD活性均表现为先抑制后激活趋势,GSH和HSP70含量无明显变化;相同暴露浓度下,随着PCP暴露时间的延长,SOD活性均表现为先激活后抑制趋势,GSH和HSP70含量无明显变化.初步认定相对于GSH、HSP70含量,SOD活性的变化可以更有效地反映低浓度PCP对稀有鮈鲫的毒性效应.
关键词(KeyWords)： 稀有鮈鲫;PCP;SOD;GSH;HSP70
基金项目(Foundation): 三峡库区生态环境教育部重点实验室科学研究基金;; 全国优秀博士学位论文作者基金(No.200355)
作者(Author): 王辅明;朱祥伟;马永鹏;刘树深;刘堰;
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