农药对土壤微生物群落的副作用的研究方法Methods for Detecting Side-Effect of Pesticides to Soil Microbial Communities
李少南;
摘要(Abstract):
微生物群落在保持土壤肥力和对外界适应能力方面起着十分重要的作用.随着农药的广泛应用和农药评估体系的建立与完善,人们越来越关注农药对土壤微生物群落的负面影响,并且尝试用多种方法进行研究.虽然对其中任何一种方法的使用,均有助于提高人们对农药副作用的认识,但是使用少数几种方法所获得的信息,已能够满足风险评估的最低要求.论文以农药风险评估的"资料要求"为依据,对"资料要求"中涉及的"碳转化"和"氮转化"两种室内试验的特点和设计思路进行了分析.此外还简要介绍了国内外农药管理部门在土壤微生物风险评估中常用的几种模型,并探讨了这些模型与具体试验方法之间的关联.从文中可以看出,对于农药的土壤微生物影响,现已建立起了比较规范化的室内测定方法,目前缺乏的是与风险评估模型相配套的田间试验方法或准则.
关键词(KeyWords): 农药;土壤微生物群落;副作用;方法学
基金项目(Foundation): 浙江省自然科学基金(No.Y307448);; 浙江省教育厅科研项目(No.20070138)
作者(Author): 李少南;
Email:
DOI:
参考文献(References):
- Cai D J,Jiang X L,Cai Y Q.1986.Safety assessment ofchemical pesticides for eco-environment-I.Effects and assessmentof chemical pesticides on soil microbes[J].Rural Eco-Environment,(2):9-13(in Chinese)
- Ehle H.1993.Side-effects on soil microflora[A].//BBA(BiologischeBundesanstalt für Land-und Forstwirtschaft,Berlin-Dahlem).Criteria for assessment of plant protection products in the registrationprocedure[C],Berlin und Hamburg:Kommissionsveriag PaulParey,84-86
- EPA(United States Environmental Protection Agency).1996.Ecological effects test guidelines OPPTS850.5100Soil microbialcommunity toxicity test[S].Washington,D.C.:EPA PublicAccess Gopher712-C-96-161
- EPPO(European and Mediterranean Plant Protection Organization).2002.Normes OEPP/EPPO Standards PP3/7(2)Environmentalrisk assessment scheme for plant protection products,Chapter8:Soil organisms and functions[S].Paris:EPPO
- FAO(Food and Agricultural Organization of the United Nations).1981.Second expert consultation on environmental criteria forregistration of pesticides[R].FAO Plant Production andProtection Paper28.Rome:FAO Sales Agents,1-60
- FAO(Food and Agriculture Organization of the United Nations).1989.Revised guidelines on environmental criteria for the registrationof pesticides[Z].Rome:FAO Sales Agents,1-47
- Johnen B G.1977.Ecological effects of pesticides on soilmicroflora[J].Soil Science,123(5):319-324
- Institute of Soil Science,Chinese Academy of Sciences.1985.Method of Soil Microorganisms-Determination of Soil Respiration[M].Beijing:Science Press,216-222(in Chinese)
- Locke M A,Zablotowicz R M.2004.Pesticides in soils-benefitsand limitations soil health[A].//Schjonning P,Elmholt S,Christensen B T.Managing Soil Quality:Challenges in ModernAgriculture[C].Oxfordshire,UK:CAB International,239-259
- Ministry of Environmental Protection of People’s Republic ofChina.1989.Guidelines on the Safety Assessment ChemicalPesticides[Z].Beijing:Ministry of Environmental Protection ofPeople’s Republic of China(in Chinese)
- OECD/OCDE(Organization for Economic Cooperation andDevelopment).2000a.OECD guideline for the testing ofchemicals216Soil microorganisms:nitrogen transformation test[S].Paris:OECD
- OECD/OCDE(Organization for Economic Cooperation andDevelopment).2000b.OECD guideline for the testing ofchemicals217Soil microorganisms:carbon transformation test[S].Paris:OECD
- OEHHA(Office of Environmental Health Hazard Assessment).2008.Soil toxicity and bioassessment test methods for ecologicalrisk assessmen[tZ].Sacramento,CA,USA:California EnvironmentalProtection Agency,16-39
- Soulas G,Lors C.1999.Perspectives and limitations in ssessingside-effects of pesticides on the soil microflora[A].//Bell C R,Brylinsky M,Johnson-Green P.Proceedings of the8thInternational Symposium on Microbial Ecology,Halifax,Canada:Atlantic Canada Society for Microbial Ecology,1-6
- Yan C S.1988.Soil Fertility Research Methods-Determination ofAmmonification and Nitrification in Soil[M].Beijing:AgriculturalPress,218-224(in Chinese)
- 蔡道基,江希流,蔡玉琪.1986.化学农药对生态环境安全评价研究—I.化学农药对土壤微生物的影响与评价[J].农村生态环境,(2):9-13
- 严昶生.1988.土壤肥力研究方法土壤氨化和硝化作用测定[M].北京:农业出版社,218-224
- 中科院南京土壤研究所微生物室.1985.土壤呼吸量测定土壤微生物研究法[M].北京:科学出版社,216-222
- 国家环境保护局.1989.化学农药安全评价试验准则[Z].北京:国家环境保护局