侯天芳;王广发;陈乾华;

• 1:航空总医院呼吸科
• 2:北京大学第一医院呼吸和危重症科

摘要(Abstract)：

大气颗粒物对肺免疫系统有潜在毒性作用,打破免疫系统平衡,大气颗粒物成分中危害首当其冲的是大气细颗粒物(PM_(2.5)),为研究大气细颗粒物引起的机体T淋巴细胞中Th1/Th2免疫失衡方向。本研究通过免疫组化实验方法检测暴露后小鼠肺组织中T淋巴细胞的表达,进一步采用流式细胞术检测大气细颗粒物气管滴注后小鼠肺脏淋巴细胞中Th1/Th2比例。暴露组小鼠肺组织免疫组化研究结果提示浸润细胞区有大量的CD4~+T细胞,中高剂量暴露组小鼠肺组织中淋巴细胞亚群Th1/Th2比例向Th1偏移。大气细颗粒物影响免疫失衡,使T淋巴细胞向Th1漂移。

关键词(KeyWords)： 大气细颗粒物;CD4~+T细胞;免疫失衡;流式细胞术

Abstract:

Keywords:

基金项目(Foundation): 北京市自然科学基金重点项目(No.7161013)

作者(Author): 侯天芳;王广发;陈乾华;

Email:

参考文献(References)：

• [1] Guo Y,Li S,Tian Z,et al. The burden of air pollution on years of life lost in Beijing,China,2004-08:Retrospective regression analysis of daily deaths[J]. British Medical Journal,2013,347:1-10
• [2] Kariisa M,Foraker R,Pennell M,et al. Short-and long-term effects of ambient ozone and fine particulate matter on the respiratory health of chronic obstructive pulmonary disease subjects[J]. Archives of Environmental&Occupational Health,2015,70:56-62
• [3] Ghasemi Z,Varasteh A R,Moghadam M,et al. Sublingual immunotherapy with Sal k1 expressing lactococcus lactis down-regulates Th2 immune responses in Balb/c mice[J]. Iranian Journal of Allergy,Asthma,and Immunology,2018,17(3):281-290
• [4] Rich D Q,Kipen H M,Huang W,et al. Association between changes in air pollution levels during the Beijing Olympics and biomarkers of inflammation and thrombosis in healthy young adults[J]. The Journal of the American Medical Association,2012,307(19):2068-2078
• [5] Zhang J,Zhu T,Kipen H,et al. Cardiorespiratory biomarker responses in healthy young adults to drastic air quality changes surrounding the 2008 Beijing Olympics[J]. Research Report(Health Effects Institute),2013,174:5-174
• [6] Hou T,Liao J,Cheng Z,et al. Elevated expression of miR-146,miR-139 and miR-340 involved in regulating Th1/Th2 balance with acute exposure of fine particulate matter in mice[J]. International Immunopharmacology,2018,54:68-77
• [7]刘瑞婷,韩志伟,李嘉伟.北京冬季雾霾事件的气象特征分析[J].气候与环境研究,2014(2):164-172Liu R T,Han Z W,Li J W. Meteorological characteristics of winter fog and haze events in Beijing[J]. Climatic and Environmental Research,2014(2):164-172(in Chinese)
• [8] Wang H,Zhuang Y,Wang Y,et al. Long-term monitoring and source apportionment of PM2.5/PM10in Beijing,China[J]. Journal of Environmental Sciences,2008,20(11):1323-1327
• [9] Zhang J J,McCreanor J E,Cullinan P,et al. Health effects of real-world exposure to diesel exhaust in persons with asthma[J]. Research Report(Health Effects Institute),2009,138:5-109
• [10]史宇,张建辉,罗海江,等.北京市2012-2013年秋冬季大气颗粒物污染特征分析[J].生态环境学报,2013(9):1571-1577Shi Y,Zhang J H,Luo H J,et al. Characteristics of atmospheric particulate matter pollution in Beijing in autumn and winter in 2012-2013 years[J]. Ecology and Environmental Sciences,2013(9):1571-1577(in Chinese)
• [11] Song C,He J,Wu L,et al. Health burden attributable to ambient PM2.5in China[J]. Environmental Pollution,2017,223:575-586
• [12] Lu Y,Su S,Jin W,et al. Characteristics and cellular effects of ambient particulate matter from Beijing[J].Environmental Pollution,2014,191:63-69
• [13] Zhang L W,Chen X,Xue X D,et al. Long-term exposure to high particulate matter pollution and cardiovascular mortality:A 12-year cohort study in four cities in Northern China[J]. Environment International,2014,62:41-47
• [14] Shang Y,Zhu T,Lenz A G,et al. Reduced in vitro,toxicity of fine particulate matter collected during the2008 summer Olympic Games in Beijing:The roles of chemical and biological components[J]. Toxicology in Vitro,2013,27(7):2084-2093
• [15] Farina F,Sancini G,Mantecca P,et al. The acute toxic effects of particulate matter in mouse lung are related to size and season of collection[J]. Toxicology Letters,2011,202(3):209-217
• [16] Lodovici M,Bigagli E. Oxidative stress and air pollution exposure[J]. Journal of Toxicology,2011,2011:487074
• [17] Ebisu K,Bell M L. Airborne PM2.5chemical components and low birth weight in the northeastern and midAtlantic regions of the United States[J]. Environmental Health Perspectives,2012,120:1746-1752
• [18] Chaudhari P R,Gupta R,Gajghate D G,et al. Heavy metal pollution of ambient air in Nagpur City[J]. Environmental Monitoring and Assessment, 2012, 184:2487-2496
• [19] Ergenekon P,UlutasK. Heavy metal content of total suspended air particles in the heavily industrialized town of Gebze,Turkey[J]. Bulletin of Environmental Contamination&Toxicology,2014,92:90-95
• [20] Pan Y,Tian S,Li X,et al. Trace elements in particulate matter from metropolitan regions of Northern China:Sources,concentrations and size distributions[J]. Science of the Total Environment,2015,537:9-22
• [21] Qiao L,Cai J,Wang H,et al. PM2.5 constituents and hospital emergency-room visits in Shanghai,China[J].Environmental Science&Technology,2014,48(17):10406-10414
• [22] Sarnat J A,Marmur A,Klein M,et al. Fine particle sources and cardiorespiratory morbidity:An application of chemical mass balance and factor analytical sourceapportionment methods[J]. Environmental Health Perspectives,2008,116(4):459-466
• [23] Agoro R,Benmerzoug S,Rose S,et al. An iron-rich diet decreases the mycobacterial burden and correlates with hepcidin upregulation,lower levels of proinflammatory mediators,and increased T-cell recruitment in a model of mycobacterium bovis bacille calmette-guerin infection[J]. The Journal of Infectious Diseases,2017,216(7):907-918
• [24] Rahman M T,Karim M M. Metallothionein:A potential link in the regulation of zinc in nutritional immunity[J]. Biological Trace Element Research,2018,182(1):1-13
• [25] Liang P,Peng S,Zhang M,et al. Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model[J]. Bioscience Reports,2017,37(6):BSR20171071
• [26] Li Q,Zhang H,Yu L,et al. Down-regulation of Notch signaling pathway reverses the Th1/Th2 imbalance in tuberculosis patients[J]. International Immunopharmacology,2018,54:24-32
• [27] Chang X,Zhu A,Liu F,et al. Role of NF-κB activation and Th1/Th2 imbalance in pulmonary toxicity induced by nano Ni O[J]. Environmental Toxicology,2017,32(4):1354-1362
• [28] Gu X Y,Chu X,Zeng X L,et al. Effects of PM2.5exposure on the Notch signaling pathway and immune imbalance in chronic obstructive pulmonary disease[J]. Environmental Pollution(Barking,Essex:1987),2017,226:163-173
• [29] Chakir H,Wang H,Lefebvre D E,et al. T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations:Predominant role of GATA-3[J]. Journal of Immunological Methods,2003,278(1-2):157-169
• [30] Park E J,Roh J,Kim Y,et al. PM 2.5 collected in a residential area induced Th1-type inflammatory responses with oxidative stress in mice[J]. Environmental Research,2011,111(3):348-355
• [31] Yoshizaki K,Brito J M,Toledo A C,et al. Subchronic effects of nasally instilled diesel exhaust particulates on the nasal and airway epithelia in mice[J]. Inhalation Toxicology,2010,22(7):610-617
• [32] Zhao J,Xie Y,Qian C,et al. Imbalance of Th1 and Th2 cells in cardiac injury induced by ambient fine particles[J]. Toxicology Letters,2012,208:225-231