3种酚类化合物对多刺裸腹溞GST和AChE活力的影响Effects of Phenolic Compounds on Glutathione-S-Transferase and Acetylcholinesterase in Moina macrocopa
张辰佳;王兰;王茜;
摘要(Abstract):
为探讨酚类化合物对水生生物的毒性效应,实验研究了多刺裸腹溞(Moina macrocopa)暴露于不同浓度的苯酚(0.25、0.75、1.25、1.75、2.25mg·L-1)、邻苯二酚(0.10、0.25、0.40、0.55、0.70mg·L-1)和间苯二酚(0.015、0.030、0.045、0.060、0.075mg·L-1)后,体内谷胱甘肽-S-转移酶(GST)和乙酰胆碱酯酶(AChE)活力的动态变化.结果表明,3种酚类化合物处理24h后,多刺裸腹溞体内GST活力随染毒浓度的升高呈先升高后降低趋势.处理48h后,体内GST活力随苯酚和邻苯二酚浓度的升高逐渐降低,而间苯二酚对GST活力的影响与处理24h后GST活力的变化趋势相似.3种酚类化合物处理24h、48h后,多刺裸腹溞体内AChE活力均呈先升高后降低趋势.48h处理多刺裸腹溞体内GST和AChE活力均显著低于24h处理.短时间内低浓度酚类化合物对GST和AChE活力具有诱导作用,但随着酚类化合物处理浓度的升高和处理时间的延长酶活力受到显著抑制.GST和AChE活力的变化能够灵敏地反映出酚类化合物对多刺裸腹溞的毒性大小及造成的损伤,初步推断可作为生物标志物来评价酚类污染物对水生生物的生化毒性.
关键词(KeyWords): 多刺裸腹溞;酚类化合物;谷胱甘肽-S-转移酶;乙酰胆碱酯酶
基金项目(Foundation): 山西省青年科技基金资助项目(No.20051035)
作者(Author): 张辰佳;王兰;王茜;
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DOI:
参考文献(References):
- Avilez I M,Hori T S,De Almeida L C,Hackbarth A,DaCunha Bastos Neto J,Da Cunha Bastos V L,Moraes G.2008.Effects of phenol in antioxidant metabolism in matrinx觔,Bryconamazonicus(Teleostei;Characidae)[J].Comparative Biochemistryand Physiology.Toxicology Pharmacology,148(2):136-142
- Barata C,Varo I,Navarro J C,Arun S,Porte C.2005.Antioxidantenzyme activities and lipid peroxidation in the freshwater cladoceranDaphnia magna exposed to redox cycling compounds[J].Comparative Biochemistry and Physiology.Toxicology Pharmacology,140(2):175-186
- Blum D J W,Speece R E.1990.Determining chemical toxicityto aquatic species[J].Environmental Science&Technology,24(3):284-293
- Calabrese E J,Baldwin L A.2003.Inorganics and hormesis[J]Critical Reviews in Toxicology,33(3):215-304
- Chen B J,Chen M S.1990.Acute toxicity of arsenic,phenol,mercury and chromium to the larvae of Penaeus Orientali[J].Marine Sciences,14(3):51-53(in Chinese)
- Chen J R.1996.Hydrochemistry[M].Beijing:Chinese AgriculturePress(in Chinese)
- Chen J Z,Leng C M,Hu G D,Qu J H.2008.Effects ofcontinuous exposure to deltamethrin on the acetylcholinesteraseactivity in blood serum of tilapia[J].Chinese Journal ofEcology,27(5):776-779(in Chinese)
- Dos Santos Miron D,Crestani M,Rosa Shettinger M,MariaMorsch V,Baldisserotto B,Angel Tierno M,Moraes G,VieiraV L.2005.Effects of the herbicides clomazone,quinclorac,andmetsulfuron methyl on acetylcholinesterase activity in the silvercatfish(Rhamdia quelen)(Heptapteridae)[J].Ecotoxicology andEnvironmental Safety,61(3):398-403
- Gao W,Liu J F,Li S W.2000.Systematic research on theacute toxicity of phenol and its substitutes[J].Journal of SuzhouInstitute of Urban Construction and Environmental Protection,13(2):32-36(in Chinese)
- George S G.1994.Enzymology and molecular biology of phaseII xenobiotic-conjugating enzymes in fish[A]//Malins D C,Ostrander G K.Aquatic Toxicology;Molecular,Biochemical andCellular Perspectives[C].Washington D C:Lewis Publishers,CRC Press,37-85
- Guo J,Gao J F,Tang Z H.2007.Kinetic mechanism andapplication on acetylcholinesterase[J].Agrochemicals,46(1):18-28(in Chinese)
- Hansch C,McKarns S C,Smith C J,Doolittle D J.2000.Comparative QSAR evidence for a free-radical mechanism ofphenol-induced toxicity[J].Chemico-Biological Interactions,127(1):61-72
- Jemec A,Drobne D,Tisler T,Trebse P,Ros M,Sepcic'K.2007.The applicability of acetylcholinesterase and glutathione S-transferase in Daphnia magna toxicity test[J].ComparativeBiochemistry and Physiology.Toxicology Pharmacology,144(4):303-309
- Li S N,Xie X C,Tan Y J,Zhu G N.2005.Induction ofTriazophos to brain acetylcholinesterase from topmouth gudgeon,Pseudorasbora parva[J].Chinese Journal of Pesticide Science,7(1):59-62(in Chinese)
- Lushchak V I,Lushchak L P,Mota A A,Hermes-Lima M.2001.Oxidative stress and antioxidant defenses in goldfishCarassius auratus during anoxia and reoxygenation[J].AmericanJournal of Physiology-Regulatory Integrative and ComparativePhysiology,280(1):100-107
- Petrusek A.2002.Moina(Crustacea:Anomopoda,Moinidae)inthe czech republic:a review[J].Acta Societatis ZoologicaeBohemicae,66:213-220
- Qu J H,Chen J Z,Hu G D,Wu W.2006.Dynamic changesof catalase and glutathione-s-transferase in the different tissues ofTilapia exposed to phenol[J].Ecology and Environment,15(4):687-692(in Chinese)
- Schultz T W,Holcombe G W,Phipps G L.1986.Relationshipsof quantitative structure-activity to comparative toxicity ofselected phenols in the Pimephales promelas and Tetrahymenapyriformis test systems[J].Ecotoxicology and EnvironmentalSafety,12(2):146-153
- State Environmental Protection Administration People’s Republicof China.1991.GB/T13266-91Water Quality—Determination ofthe Acute Toxicity of Substance to Daphnia(Daphnia magnastraus)[S].Beijing:State Environmental Protection AdministrationPeople’s Republic of China(in Chinese)
- The Editorial Board of Standard Methods for Water andWastewater Monitoring and Analysis.2002.Standard Methods forWater and Wastewater Monitoring and Analysis(4th Edition)[M].Beijing:China Environmental Science Press
- Van der Oost R,Beyer J,Vermeulen N P E.2003.Fishbioaccumulation and biomarkers in environmental risk assessment:a review[J].Environmental Toxicology and Pharmacology,13(2):57-149
- Wei A H,Xu H,Zhu Y.2001.Accumulation,distribution andrelease of phenolate in Carps(Cyprinus carpio Linn)[J].Fisheries Science,20(1):12-14(in Chinese)
- Wu W,Chen J Z,Leng C M,Tong X.2006.The effect ofdeltamethrin on glutathione and giutathione-S-transferase in thebody of Tilapia[J].China Environmental Science,26(4):474-477(in Chinese)
- Yang H L,Nie L J,Zhu S G,Zhou X W.2006.Structure andcatalytic mechanism of the glutathione transferasea[J].Journal ofChengdu University(Natural Science Edition),25(1):19-24(inChinese)
- Yu R L,Zhao Y H,Hu G R.2003.Acute toxicity of phenolchemicals to Daphnia magna at different pH values and theQSAR study[J].Journal of Huaqiao University(Natural Science),24(1):44-47(in Chinese)
- Zhang M,Li D,Chen Y B,Fang F,Luo X T.2006.Researchprogress in acetylcholinesterase molecular biology[J].Agrochemicals,45(1):8-11(in Chinese)
- Zhang N,Zhou Q X,Li T,Luo Y.2008.Ecotoxic effects ofoxidation hair dyes on Perinereis aibuhiteusis grube and itsenzyme activities[J].Asian Journal of Ecotoxicology,3(1):65-71(in Chinese)
- Zhou Q F,Fu J J,Meng H Z,Zhu X Y,Jiang G B,Zhang JB,Liu J M,Shi G Q.2007.Subacute toxic effects ofnitrobenzene in water on the Oryzias latipes and Gobiocyprisrarus[J].Science in China(Series B:Chemistry),37(2):197-206(in Chinese)
- 陈碧娟,陈民山.1990.汞、砷、铬、苯酚对东方对虾幼体急性致毒的实验研究[J].海洋科学,14(3):51-53
- 陈佳荣.1996.水化学[M].北京:中国农业出版社
- 陈家长,冷春梅,胡庚东,瞿建宏.2008.溴氰菊酯连续暴露对罗非鱼血清乙酰胆碱酯酶活性的影响[J].生态学杂志,27(5):776-779
- 高为,刘江锋,李绍伟.2000.苯酚类毒物的毒性研究[J].苏州城建环保学院学报,13(2):32-36
- 郭晶,高菊芳,唐振华.2007.乙酰胆碱酯酶的动力学机制及其应用[J].农药,46(1):18-28
- 国家环境保护总局.1991.GB/T13266-91水质物质对溞类(大型溞)急性毒性测定方法[S].北京:国家环境保护总局
- 李少南,谢显传,谭亚军,朱国念.2005.三唑磷对麦穗鱼脑组织中乙酰胆碱酯酶的诱导[J].农药学学报,7(1):59-62
- 瞿建宏,陈家长,胡庚东,吴伟.2006.苯酚胁迫下罗非鱼组织中过氧化氢酶与谷胱甘肽-S-转移酶的动态变化[J].生态环境,15(4):687-692
- 水和废水监测分析方法编委会.2002.水和废水监测分析方法(第4版)[M].北京:中国环境科学出版社
- 魏爱泓,徐虹,朱毅.2001.鲤对酚的积累、分配和释放研究[J].水产科学,20(1):12-14
- 吴伟,陈家长,冷春梅,佟欣.2006.溴氰菊酯对罗非鱼谷胱甘肽及S转移酶的影响[J].中国环境科学,26(4):474-477
- 杨海灵,聂力嘉,朱圣庚,周先碗.2006.谷胱甘肽硫转移酶结构与功能研究进展[J].成都大学学报(自然科学版),25(1):19-24
- 于瑞莲,赵元慧,胡恭任.2003.酚类对大型溞的急性毒性及QSAR研究[J].华侨大学学报(自然科学版),24(1):44-47
- 张明,李盾,陈仪本,方峰,罗雪桃.2006.乙酰胆碱酯酶分子生物学研究进展[J].农药,45(1):8-11
- 张宁,周启星,李婷,罗义.2008.氧化型染发剂对沙蚕的毒性效应及对部分酶活性的影响[J].生态毒理学报,3(1):65-71
- 周群芳,傅建捷,孟海珍,朱学艳,江桂斌,张建斌,刘杰民,时国庆.2007.水体硝基苯对日本青鳉和稀有鮈鲫的亚急性毒理学效应[J].中国科学(B辑:化学),37(2):197-206