朱枫;钱晨;卢彦;

• 1:南京大学生命科学学院医药生物技术国家重点实验室

摘要(Abstract)：

微囊藻毒素对生物机体具有强烈的毒性.近几年的研究表明,微囊藻毒素能够诱导细胞产生氧化胁迫,这可能是微囊藻毒素的致毒机理的一个重要方面.在总结国内外相关研究基础上,论文从微囊藻毒素诱导细胞氧化损伤以及影响其抗氧化防御系统两个方面综述了微囊藻毒素诱导细胞氧化应激的研究进展.

关键词(KeyWords)： 微囊藻毒素;氧化胁迫;致毒机理;抗氧化防御系;细胞氧化损伤

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(No.2008CB418102);; 教育部科学技术研究重点项目(No.107049)

作者(Author): 朱枫;钱晨;卢彦;

Email:

参考文献(References)：

• Ana I P,Angeles J,Silvia P,Isabel M,Ana M C.2008.Protective role of vitamin E on the microcystin-induced oxidative stress in tilapia fish(Oreochromis niloticusz)[J].EnvirmentaToxicology and Chemistry,27(5):1152-1159
• Ana I P,Silvia P,Angeles J,Isabel M,Ana M C.2007.Timedependent oxidative stress responses after acute exposure to toxiccyanobaterial cells containing microcystins in tilapia fish(Oreochromis niloticus)under laboratory conditions[J].AquaticToxicology,84(3):337-345
• Anja P,Shumon C,Stephan P.2007.Uptake of Microcystins LR and LF(cyanobacterial toxins)in seedings of several importantagricultural plant species and the correlation with cellular damage[J].Environmental Toxicology,22:436-442
• Carmichael W W,Azevedo S M F O,An J S,R J R Molica,Jochimsen E M,Lau S,Rinehart K L,Shaw G R,Eaglesham GK.2001.Human fatalities from cyanobacteria:chemical andbiological evidence for cyanotoxins[J].Environmental HealthPerspectives,109(7):663-668
• Chen T,Cui J,Liang Y,Xin X B,Young O,Chen C,Shen P.2006.Identification of human liver mitochondrial aldehyddehydrogenase as a potential target for microcystin-LR[J]Toxicology,220(1):71-80
• Chen T,Wang Q S,Cui J,Yang W,Shi Q,Hua Z H,Ji J Gand Shen P P.2005.Induction of apoptosis in mouse liver bmicrocystin-LR[J].Molecuar Cellular Proteomics,4(7):958-97
• Ding W X,Ong C N.2003.Role of oxidative stress andmitochondrial changes in cyanobacteria-induced apoptosis andhepatotoxicity[J].FEMS Microbiology letters,220(1):1-7
• Ding W X,Shen H M,Ong C N.2000a.Microcystic cyanobacteriaextract induces cytoskeletal disruption and intracellular glutathionealteration in Hepatocytes[J].Environmental Health Perspectives,108(7):605-609
• Ding W X,Shen H M,Ong C N.2000b.Critical role ofreactive oxygen species and mitochondrial permeability transitionin microcystin-induced rapid apoptosis in rat hepatocytes[J].Hepatology,32(3):547-555
• Ding W X,Shen H M,Ong C N.2001a.Critical role ofreactive oxygen species formation in mc-induced cytoskeletondisruption in primary cultured hepatocytes[J].Journal ofToxicology and Environmental Health A,285:507-519
• Ding W X,Shen H M,Ong C N.2001b.Pivotal role ofmitochondrial Ca in microcystin-induced mitochondrial permeabilitytransition in rat hepatocytes[J].Biochemical and BiophysicalResearch Communications,285(5):1155-1161
• Ding W X,Shen H M,Ong C N.2002.Calpain activation aftermitochondrial permeability transition in microcystin-induced celldeath in rat hepatocytes[J].Biochemical and BiophysicalResearch Communications,291(2):321-331
• Ding W X,Shen H M,Zhu H G,Ong C N.1998.Hepatotoxicity and formation of reactive oxygen species inducedby Microcystis aeruginos toxin[J].Chinese Journal of PreventiveMedicine,32(5):278-280(in Chinese)
• Dong X,Jill G,Sherry M,Thomas S,William T.2006.Mitochondrial aldehyde dehydrogenase attenuates hyperoxia-inducedcell death through activation of ERK/MAPK and PI3K-Akt pathwaysin lung epithelial cells[J].American Journal of Physiology.Lung Cell Molecular Physiology,291:L966-L975
• Fu W Y,Xu L H.2006.Progress in mechanism of microcystintoxicity[J].Journal of Zhejiang University(Medical Sciences),35(3):342-346(in Chinese)
• Guzman R E,Solter P F.1999.Hepatic oxidative stress followingprolonged sublethal microcystin LR exposure[J].ToxicologicPathology,27:582-588
• Hu Z Q,Liu Y D,Li D H,Dauta A.2005.Growth andantioxidant system of the cyanobacterium Synechococcus elongatusin response to microcystin-RR[J].Hydrobiologia,534(1-3):23-29
• Huang W M,Xing W,Li D H,Liu Y D.2007.The influenceof exogenous DMSO and the ASA to tobacco cells,ROSgeneration and antioxidant systems in response to microcystin[J].Acta Hydrobiologica Sinica,31(6):869-873(in Chinese)
• Huang W N,Xing W,Li D H,Liu Y D.2008.The role ofglutathione metabolism in tolerance of tabacco BY-2suspensioncells to microcystin-RR[J].Bulletin of Environmental Contaminationand Toxicology,80(3):215-219
• Jaeg J P.2007.Microcystins:intoxication in domestic animalsand food safety[J].Revue De Medicine Veterinaire,158(1):46-58
• Jayaraj R,Anand T,Rao P V.2006.Activity and gene expressionprofile of certain antioxidant enzymes to microcystin-LR inducedoxidative stress in mice[J].Toxicology,220(2-3):136-146
• Leaao J C,Geracitano L A,Monserrat J M,Amado L L,YunesJ S.2008.Microcystin-induced oxidative stress in Laeonereisacuta(Polychaeta,Nereididae)[J].Marine Environmental Research,66:92-94
• Li X Y,Liu Y S,Song L R,Qiao Z G.2003.The anti oxidation system in carp hepatocyte in response to the toxicity ofmicrocystin[J].Acta Hydrobiologica Sinica,27(5):472-475(inChinese)
• Li Y,Sheng J,Sha J H,Han X D.2008.The toxic effects ofmicrocystin-LR on the reproductive system of male rats in vivoand in vitro[J].Reproductive Toxicology,26(3-4):239-245
• Natarajan V,Scribuner W M.1998.Reactive oxygen speciessignaling through regulation of protein tyrosine phosphorylation inendothelial cells[J].Environmental Health Perspectives,106(Suppl.5):1205-1212
• Paual K,Elaine H,Ana C,Rui C.2008.Analysis of chemokinesand reactive oxygen species formation by rat and human neutrophilsinduced by microcystin-LA,YR and-LR[J].Toxicon,51(7):1274-1280
• Paulina S,Bozena B,Jaromir M,Wirgiliusz D.2006.Damageof cell membrane and antioxidative system in human erythrocytesindubated with microcystin-LR in vitro[J].Toxicon,47(4):387-397
• Pinho G L L,Moura D R C,Maciel F E,Bianchini A,Yunes JS,Proenca L A O,Monserrat J M.2005.Antioxidant responsesand oxidative stress after microcystin exposure in the hepatopancreasof an estuarine crab species[J].Ecotoxicology and EnvironmentalSafety,61(3):353-360
• Sampson J B,Beckman J S.2001.Hydrogen peroxide damagesthe zinc-binding site of zinc-de覱cient Cu,Zn superoxide dismutase[J].Archives of Biochemistry and Biophysics,392:8-13
• Song R X,Liu Z T,Shen P P.2004.Injury effect ofmicrocystins extracted from Taihu lake on chromosome and DNA[J].Chinese Journal of Public Health,20(12):1446-1447(inChinese)
• Stefan H,Joachim N.2000.Effects of serine/threonine proteinphosphatases on ion channels in excitable membranes[J].Physiological Reviews,80(1):173-210
• Sun Y H,Tang R,Li D P,Zhang X Z,Fu J,Xie P.2008.Acute effects of microcystins on the transcription of antioxidantenzyme genes in crucian carp Carassius auratus[J].EnvironmentalToxicology,23(2):145-152
• Wei Y N,Wang D,Li F,Zou X,Young D O,Ji J G,Shen PP.2008.Involvement of JNK regulation in oxidative stress mediated murine liver injury by microcystin-LR[J].Apaptosis,13(8):1031-1042
• Weng D,Lu Y,Wei Y N,Liu Y,Shen P P.2003.The roleof ROS in microcystin-LR-induced hepatocyte apoptosis and liverinjury in mice[J].Toxicology,232(1-2):15-23
• Yang C Y,Wang W B,Li D H,Liu Y D.2008.Growth andantioxidant system of Escherichia coli in response to microcystin RR[J].Bulletin of Environmental contamination and Toxicology,81(5):427-431
• Yin L Y,Huang J Q,Huang W M,Li D H,Liu Y D.2005.Responses of antioxidant system in Arabidopsis thaliana suspensioncells to the toxicity of microcystin-RR[J].Toxicon,46(8):859-864
• Yin L Y,Huang J Q,Huang W M,Li D H,Wang G H,LiuY D.2005.Microcystin-RR induces accumulation of reactiveoxygen species and alteration of antioxidant systems in tobaccoBY-2cells[J].Toxicon,46(5):507-512
• Zegura B,Lah T T,Filipic M.2004.The role of reactiveoxygen species in microcystin-LR-induced DNA damage[J].Toxicology,200(1):59-68
• Zegura B,Lah T T,Filipic M.2006.Alteration of intracelluarGSH levels and its role in microcystin-LR-induced DNA damagein human hepatoma HepG-2cells[J].Mutation Research,611(1-2):25-33
• Zegura B,Volcic M,Lah T T,Filipic M.2008.Different sensitivities of human colon adenocarcinoma(CaCo-2),astrocytoma(IPDDC-A2)and lymphoblastoid(NCNC)cell lines to microcystinLR induced reactive oxygen species and DNA damage[J].Toxicon,52(3):518-525
• Zhang H,Zhang J,Chen Y,Zhu Y.2007.Influence ofintracellular Ca2+,mitochondria membrane potential,reactive oxygenspecies,and intrancellular ATP on the mechanism of microcystin LR induced apoptosis in Carassius auratus lymphocytes in vitro[J].Environmental Toxicology,22(6):559-564
• Zhang H,Zhang J,Chen Y,Zhu Y.2008.Microcystin-RRinduces apoptosis in fish lymphocytes by generating reactiveoxygen species and causing mitochondrial damage[J].FishPhysiologia Biochemistry,34(4):307-312
• Zhang Z Y,Liang H J,Shen H M,Wang J N.1999.Theeffect of oxidative damage to primary cultured rat hepatocytes inreponse to the extract of Blue-green algae[J].Chinese Journalof Public Health,15(4):280-282(in Chinese)
• 丁文兴,沈汉民,朱惠刚,OngChoon Nam.1998.铜绿微囊藻毒素的肝细胞毒性及活性氧化生成作用[J].中国预防医学杂志,32(5):278-280
• 傅文宇,徐立红.2006.微囊藻毒素致毒机理的研究进展[J].浙江大学学报(医学版),35(3),342-346
• 黄文敏,邢伟,李敦海,刘永定.2007.外源DMSO和ASA对微囊藻毒素胁迫下烟草细胞ROS生成和抗氧化系统的影响[J].水生生物学报,31(6):869-873
• 李效宇,刘永定,宋立荣,乔志刚.2003.鲤肝细胞抗氧化系统对微囊藻毒素毒性的反应[J].水生生物学报,27(5):472475
• 梁艳,卢彦,沈萍萍.2007.微囊藻毒素-LR对小鼠巨噬细胞吞噬功能及活性氧水平的影响[J].中国药理学与毒理学杂志,21(1):55-58
• 宋瑞霞,刘征涛,沈萍萍.2004.太湖微囊藻毒素对细胞染色体及DNA损伤效应[J].中国公共卫生,20(12):1446-1447
• 张志勇,梁恒进,沈汉民,王俊南.1999.蓝藻提取物对原代培养大鼠肝细胞的氧化损伤作用研究[J].中国公共卫生,15(4):280-282