党红蕾;那广水;高会;李瑞婧;高艳飞;姚子伟;祖国仁;

• 1:大连工业大学生物工程学院
• 2:国家海洋局近岸海域生态环境重点实验室国家海洋环境监测中心

摘要(Abstract)：

为探究海洋环境中持久性有机污染物——全氟辛烷磺酸(PFOS)的生物毒性效应,以半滑舌鳎肝脏细胞(HTLC)为研究对象,将其暴露于含不同浓度PFOS的DMEM-F12培养基中,分别染毒24、48、72 h后,利用噻唑蓝比色法(MTT)和透射电镜实验评价PFOS的细胞毒性;同时测定活性氧自由基(ROS)和超氧化物歧化酶(SOD)活性来探讨PFOS对细胞的氧化损伤效应。结果发现,细胞活性随PFOS浓度升高呈先促进后抑制趋势,当PFOS浓度达到1 000%mol·L-1时细胞活性受到显著抑制(P<0.01);电镜结果显示PFOS能引起与代谢相关的细胞器如线粒体、内质网等发生肿胀甚至破损;与对照组相比,ROS含量和SOD活性分别在20%mol·L-1、200%mol·L-1开始出现显著性差异(P<0.05),证实在PFOS引起的氧化应激反应中SOD起到了清除自由基作用以维持细胞稳态。研究表明,PFOS对海洋鱼类细胞具有一定的生物毒性,能引起细胞产生氧化应激反应,并进一步破坏生物膜系统,从而导致细胞增殖和多种代谢途径受到抑制。

关键词(KeyWords)： 全氟辛烷磺酸;肝细胞;半滑舌鳎;细胞毒性;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家海洋局海洋公益性科研专项(201105013);; 国家自然科学基金(21377032);; 国家海洋局近岸海域生态环境重点实验室开放基金(201506)

作者(Author): 党红蕾;那广水;高会;李瑞婧;高艳飞;姚子伟;祖国仁;

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