党红蕾;那广水;高会;李瑞婧;高艳飞;姚子伟;祖国仁;

• 1:大连工业大学生物工程学院
• 2:国家海洋局近岸海域生态环境重点实验室国家海洋环境监测中心

摘要(Abstract)：

为探究海洋环境中持久性有机污染物——全氟辛烷磺酸(PFOS)的生物毒性效应,以半滑舌鳎肝脏细胞(HTLC)为研究对象,将其暴露于含不同浓度PFOS的DMEM-F12培养基中,分别染毒24、48、72 h后,利用噻唑蓝比色法(MTT)和透射电镜实验评价PFOS的细胞毒性;同时测定活性氧自由基(ROS)和超氧化物歧化酶(SOD)活性来探讨PFOS对细胞的氧化损伤效应。结果发现,细胞活性随PFOS浓度升高呈先促进后抑制趋势,当PFOS浓度达到1 000%mol·L-1时细胞活性受到显著抑制(P<0.01);电镜结果显示PFOS能引起与代谢相关的细胞器如线粒体、内质网等发生肿胀甚至破损;与对照组相比,ROS含量和SOD活性分别在20%mol·L-1、200%mol·L-1开始出现显著性差异(P<0.05),证实在PFOS引起的氧化应激反应中SOD起到了清除自由基作用以维持细胞稳态。研究表明,PFOS对海洋鱼类细胞具有一定的生物毒性,能引起细胞产生氧化应激反应,并进一步破坏生物膜系统,从而导致细胞增殖和多种代谢途径受到抑制。

关键词(KeyWords)： 全氟辛烷磺酸;肝细胞;半滑舌鳎;细胞毒性;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家海洋局海洋公益性科研专项(201105013);; 国家自然科学基金(21377032);; 国家海洋局近岸海域生态环境重点实验室开放基金(201506)

作者(Author): 党红蕾;那广水;高会;李瑞婧;高艳飞;姚子伟;祖国仁;

Email:

DOI:

参考文献(References)：

• [1]杨莉莉,金芬,李敏洁,等.食品和食品包装材料中全氟化合物(PFCs)的研究进展[J].食品工业科技,2014,35(8):367-373Yang L L,Jin F,Li M J,et al.Research progress in advance of perfluorochemicals in food[J].Science and Technology of Food Industry,2014,35(8):367-373(in Chinese)
• [2]梅胜放.我国PFOS/PFOA的生产,应用以及国内外标准现状[J].有机氟工业,2008(1):21-25
• [3]Wang T,Wang Y,Liao C,et al.Perspectives on theinclusion of perfluorooctane sulfonate into the stockholm convention on persistent organic pollutants 1[J].Environmental Science&Technology,2009,43(14):5171-5175
• [4]杨永亮,路国慧,杨伟贤,等.沈阳地区水环境和生物样品中全氟化合物的污染分布特征[J].环境科学学报,2010,30(10):2097-2107Yang Y L,Lu G H,Yang W X,et al.Levels and distribution of perfluorinated compounds in water and biological samples from the Shenyang area,China[J].Acta Scientiae Circumstantiae,2010,30(10):2097-2107(in Chinese)
• [5]潘媛媛,史亚利,蔡亚岐.土壤,底泥和活性污泥中全氟化合物的高效液相色谱-串联质谱分析方法[J].环境化学,2010,29(3):519-523Pan Y Y,Shi Y L,Cai Y Q.Determination of perfluorinated compounds in soil,sediment and sludge using HPLC-MS/MS[J].Environmental Chemistry,2010,29(3):519-523(in Chinese)
• [6]胡佳玥,戴家银.全氟及多氟类化合物在人体分布及其毒性研究进展[J].生态毒理学报,2013,8(5):650-657Hu J Y,Dai J Y.Advance instudies on human distribution and toxic effects of perfluoroalkyl and polyfluoroalkyl substances[J].Asian Journal of Ecotoxicology,2013,8(5):650-657(in Chinese)
• [7]杨兰琴,冯雷雨,陈银广.中国水环境中全氟化合物的污染水平及控制策略[J].化工进展,2012,31(10):2304-2312Yang L Q,Feng L Y,Chen Y G.Pollution status and control strategies of perfluorinated compounds in water environments over China:A review[J].Chemical Industry and Engineering Progress,2012,31(10):2304-2312(in Chinese)
• [8]韩建,方展强.水环境PFOS和PFOA的污染现状及毒理效应研究进展[J].水生态学杂志,2010,3(2):99-105
• [9]Pan Y,Shi Y,Wang Y,et al.Investigation of perfluorinated compounds(PFCs)in mollusks from coastal waters in the Bohai Sea of China[J].Journal of Environmental Monitoring,2010,12(2):508-513
• [10]Chen C,Wang T,Naile J E,et al.Perfluorinated compounds in aquatic products from Bohai Bay,Tianjin,China[J].Human and Ecological Risk Assessment:An International Journal,2011,17(6):1279-1291
• [11]Gulkowska A,Jiang Q,So M K,et al.Persistent perfluorinated acids in seafood collected from two cities of China[J].Environmental Science&Technology,2006,40(12):3736-3741
• [12]Zhao Y G,Wan H T,Law A,et al.Risk assessment for human consumption of perfluorinated compound-contaminated freshwater and marine fish from Hong Kong and Xiamen[J].Chemosphere,2011,85(2):277-283
• [13]Lin A Y C,Panchangam S C,Lo C C.The impact of semiconductor,electronics and optoelectronic industries on downstream perfluorinated chemical contamination in Taiwanese rivers[J].Environmental Pollution,2009,157(4):1365-1372
• [14]Moody C A,Martin J W,Kwan W C,et al.Monitoring perfluorinated surfactants in biota and surface water samples following an accidental release of fire-fighting foam into Etobicoke Creek[J].Environmental Science&Technology,2002,36(4):545-551
• [15]Taniyasu S,Kannan K,Horii Y,et al.A survey of perfluorooctanesulfonate and related perfluorinated organic compounds in water,fish,birds,and humans from Japan[J].Environmental Science&Technology,2003,37(12):2634-2639
• [16]Hoff P T,Van Campenhout K,Van de Vijver K,et al.Perfluorooctane sulfonic acid and organohalogen pollutants in liver of three freshwater fish species in Flanders(Belgium):Relationships with biochemical and organismal effects[J].Environmental Pollution,2005,137(2):324-333
• [17]Fang C,Huang Q,Ye T,et al.Embryonic exposure to PFOS induces immunosuppression in the fish larvae of marine Medaka[J].Ecotoxicology and Environmental Safety,2013,92:104-111
• [18]叶露,吴玲玲,蒋雨希,等.PFOS/PFOA对斑马鱼(Danio rerio)胚胎致毒效应研究[J].环境科学,2009,29(6):170-174Ye L,Wu L L,Jiang Y X,et al.Toxicological study of PFOS/PFOA to zebrafish(Danio rerio)embryos[J].Environmental Science,2009,29(6):170-174(in Chinese)
• [19]Shi X,Du Y,Lam P K S,et al.Developmental toxicity and alteration of gene expression in zebrafish embryos exposed to PFOS[J].Toxicology and Applied Pharmacology,2008,230(1):23-32
• [20]Xia J,Fu S,Cao Z,et al.Ecotoxicological effects of waterborne PFOS exposure on swimming performance and energy expenditure in juvenile goldfish(Carassius auratus)[J].Journal of Environmental Sciences,2013,25(8):1672-1679
• [21]Dorts J,Kestemont P,Marchand P A,et al.Ecotoxicoproteomics in gills of the sentinel fish species,Cottusgobio,exposed to perfluorooctane sulfonate(PFOS)[J].Aquatic Toxicology,2011,103(1):1-8
• [22]任国诚,陈松林,沙珍霞.半滑舌鳎肝脏细胞系的建立与鉴定[J].高技术通讯,2008,18(6):657-660Ren G C,Chen S L,Sha Z X.Development and characterization of a liver cell line from half-smooth tongue-sole(Cynoglossus semilaevis)[J].Chinese High Technology Letters,2008,18(6):657-660(in Chinese)
• [23]李龙,陈家堃.现代毒理学实验技术原理与方法[M].北京:化学工业出版社,2006:79
• [24]沈葆真,曹文军,钟键.全氟化合物在生物体内污染水平的研究进展[J].化学与生物工程,2014,31(1):6-9
• [25]Bao J,Krrman A,van Bavel B,et al.Perfluoroalkyl substances in the blood samples from a male population of Sweden[J].Chinese Science Bulletin,2014,59(4):388-395
• [26]刘晓晖,胡宏,李双月,等.全氟辛烷磺酸神经发育毒性机制研究进展[J].生态毒理学报,2013,8(5):643-649Liu X H,Hu H,Li S Y,et al.Research progress onmechanisms in the developmental neurotoxicity of PFOS[J].Asian Journal of Ecotoxicology,2013,8(5):643-649(in Chinese)
• [27]Pereiro N,Moyano R,Blanco A,et al.Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic-pituitary-adrenal axis in adult male rats[J].Toxicology Letters,2014,230(2):252-262
• [28]Liu C,Du Y,Zhou B.Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes[J].Aquatic Toxicology,2007,85(4):267-277
• [29]Kienzler A,Bony S,Devaux A.DNA repair activity in fish and interest in ecotoxicology:A review[J].Aquatic Toxicology,2013,134:47-56
• [30]端正花,王勋功,王华.全氟辛烷羧酸(PFOA)与全氟辛烷磺酸(PFOS)的细胞毒性效应[J].生态毒理学报,2014,9(2):353-357Duan Z H,Wang X G,Wang H.The cytotoxic effect of PFOA and PFOS[J].Asian Journal of Ecotoxicology,2014,9(2):353-357(in Chinese)
• [31]Florentin A,Deblonde T,Diguio N,et al.Impacts of two perfluorinated compounds(PFOS and PFOA)on human hepatoma cells:Cytotoxicity but no genotoxicity?[J].International Journal of Hygiene and Environmental Health,2011,214(6):493-499
• [32]Shan G,Ye M,Zhu B,et al.Enhanced cytotoxicity of pentachlorophenol by perfluorooctanesulfonate or perfluorooctanoic acid in Hep G2 cells[J].Chemosphere,2013,93(9):2101-2107
• [33]Reuter S,Gupta S C,Chaturvedi M M,et al.Oxidative stress,inflammation,and cancer:How are they linked?[J].Free Radical Biology and Medicine,2010,49(11):1603-1616
• [34]张晶,梁岳,方展强.全氟辛烷磺酸类物质对剑尾鱼Cu/Zn-SOD及相关应激基因表达的影响[J].水产学报,2014,38(4):482-490
• [35]王贺威,马胜伟,张喆,等.全氟辛烷磺酸盐(PFOS)胁迫对翡翠贻贝抗氧化酶的影响[J].生态毒理学报,2012,7(5):508-516Wang H W,Ma S W,Zhang Z,et al.Effects of perfluorooctane sulfonate(PFOS)exposure on antioxidant enzymes of Perna viridis[J].Asian Journal of Ecotoxicology,2012,7(5):508-516(in Chinese)
• [36]Wang X,Zhao G,Liang J,et al.PFOS-induced apoptosis through mitochondrion-dependent pathway in human-hamster hybrid cells[J].Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2013,754(1):51-57
• [37]Klaunig J E,Kamendulis L M.The role of oxidative stress in carcinogenesis[J].Annual Review of Pharmacology and Toxicology,2004,44:239-267
• [38]Orrenius S,Nicotera P,Zhivotovsky B.Cell death mechanisms and their implications in toxicology[J].Toxicological Sciences,2011,119(1):3-19
• [39]Zhang D Y,Xu X L,Shen X Y,et al.Analysis of apoptosis induced by perfluorooctane sulfonates(PFOS)in mouse leydig cells in vitro[J].Toxicology Mechanisms and Methods,2015,25(1):21-25