代谢活化作用对多溴代联苯类污染物类/抗甲状腺激素活性的影响Infection on Thyroid Hormone Agonistic and Antagonistic Activation of Polybrominated Diphenyl Ether by in Vitro Metabolism
刘芸;李剑;马梅;王子健;饶凯锋;张玉秀;
摘要(Abstract)：
选用大鼠肝匀浆(S9)和鼠肝癌细胞(H4ⅡE)两种体系代谢活化多溴代联苯醚混标(BDEs)和十溴联苯醚(BDE209),采用重组甲状腺激素受体基因酵母检测BDEs和BDE209母体及其代谢产物的类/抗甲状腺激素效应.结果表明,BDEs和BDE209母体均不表现甲状腺激素效应(p>0.05);但是经S9和H4ⅡE细胞代谢活化后,其代谢产物表现出明显的类甲状腺激素活性和抗甲状腺激素活性(p<0.05),BDEs和BDE209的干扰甲状腺激素效应需要经过代谢活化步骤.比较不同代谢活化体系,重组酵母细胞本身的代谢活化作用并不显著,而H4ⅡE细胞和S9代谢活化体系均能够导致活性中间体.
关键词(KeyWords)： 多溴联苯醚;重组酵母;S9;H4ⅡE;甲状腺激素受体;体外代谢
基金项目(Foundation): 国家自然科学基金项目(No.50778170);; 国家高技术研究发展计划(863)项目(No.2007AA06Z414)
作者(Author): 刘芸;李剑;马梅;王子健;饶凯锋;张玉秀;
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