朱飞龙;郝伟玉;尹晶;常静;李济彤;李建中;李伟;王会利;

• 1:中国科学院生态环境研究中心
• 2:中国科学院大学

摘要(Abstract)：

腈菌唑(myclobutanil,MT)是一种应用广泛的手性杀菌剂,其手性对映体为(+)-腈菌唑(MT1)和(-)-腈菌唑(MT2)。腈菌唑手性单体具有不同的生物活性,但很少有其对爬行动物的对应选择毒性研究。为了评估腈菌唑手性单体对丽斑麻蜴性腺系统的毒性影响,将MT1(50 mg·kg-1)和MT2(50 mg·kg-1)以经口灌胃方式分别暴露给蜥蜴28 d。暴露期间,记录观察蜥蜴体重、血液中性激素(睾酮T和雌二醇E2)浓度以及性腺相关基因(3β-HSD、17β-HSD、CYP11A、CYP17、CYP19A、ER-α和AR)的表达图谱的变化。在MT1暴露组中,蜥蜴体重在28 d出现明显下降。而在MT2暴露组中,蜥蜴体重没有明显变化。雄性蜥蜴在经过1 d的暴露后,E2浓度在MT1暴露组中明显下降,而在MT2暴露组中明显上升。这些结果表明了腈菌唑手性单体的对映选择性毒性。在MT2暴露14 d后,雌性蜥蜴中CYP19A基因的表达上调弥补了体内E2浓度的降低。在MT1暴露的14 d,蜥蜴卵巢中观察到的CYP11A和CYP17基因表达下调以及CYP19A基因表达不变的这一现象解释了蜥蜴血液中E2和T的浓度减少。这些结果表明:MT1和MT2暴露对蜥蜴体内性激素造成不同的影响,而这些影响会相应改变性腺相关基因的表达。综上所述,MT1和MT2对性腺系统具有潜在的内分泌干扰效应,可能对蜥蜴造成不同程度的生殖损伤。

关键词(KeyWords)： 腈菌唑;蜥蜴;内分泌干扰;基因表达图谱

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21477152)

作者(Author): 朱飞龙;郝伟玉;尹晶;常静;李济彤;李建中;李伟;王会利;

Email:

参考文献(References)：

• [1]Goetz A K,Ren H,Schmid J E,et al.Disruption of testosterone homeostasis as a mode of action for the reproductive toxicity of triazole fungicides in the male rat[J].Toxicological Sciences,2007,95(1):227-239
• [2]Yu L,Chen M,Liu Y,et al.Thyroid endocrine disruption in zebrafish larvae following exposure to hexaconazole and tebuconazole[J].Aquatic Toxicology,2013,138-139:35-42
• [3]Li J,Chang J,Li W,et al.Disruption of sex-hormone levels and steroidogenic-related gene expression on Mongolia Racerunner(Eremias argus)after exposure to triadimefon and its enantiomers[J].Chemosphere,2017,171:554-563
• [4]Lewis D L,Garrison A W,Wommack K E,et al.Influence of environmental changes on degradation of chiral pollutants in soils[J].Nature,1999,401(6756):898-901
• [5]Cheng C,Huang L,Diao J,et al.Enantioselective toxic effects and degradation of myclobutanil enantiomers in Scenedesmus obliquus[J].Chirality,2013,25(12):858-864
• [6]Dong F,Li J,Chankvetadze B,et al.Chiral triazole fungicide difenoconazole:Absolute stereochemistry,stereoselective bioactivity,aquatic toxicity,and environmental behavior in vegetables and soil[J].Environmental Science&Technology,2013,47(7):3386-3394
• [7]Li Y,Dong F,Liu X,et al.Chiral fungicide triadimefon and triadimenol:Stereoselective transformation in greenhouse crops and soil,and toxicity to Daphnia magna[J].Journal of Hazardous Materials,2014,265:115-123
• [8]Yan J,Zhang P,Wang X,et al.Stereoselective degradation of chiral fungicide myclobutanil in rat liver microsomes[J].Chirality,2014,26(1):51-55
• [9]Rockett J C,Narotsky M G,Thompson K E,et al.Effect of conazole fungicides on reproductive development in the female rat[J].Reproductive Toxicology,2006,22(4):647-658
• [10]Goetz A K,Dix D J.Toxicogenomic effects common to triazole antifungals and conserved between rats and humans[J].Toxicology and Applied Pharmacology,2009,238(1):80-89
• [11]Kjaerstad M B,Taxvig C,Nellemann C,et al.Endocrine disrupting effects in vitro of conazole antifungals used as pesticides and pharmaceuticals[J].Reproductive Toxicology,2010,30(4):573-582
• [12]Okubo T,Yokoyama Y,Kano K,et al.Estimation of estrogenic and antiestrogenic activities of selected pesticides by MCF-7 cell proliferation assay[J].Archives of Environmental Contamination and Toxicology,2004,46(4):445
• [13]Guillette L J,Crain D A,Gunderson M P,et al.Alligators and endocrine disrupting contaminants:A current perspective 1[J].American Zoologist,2000,40(3):438-452
• [14]Kanaho Y I,Endo D,Park M K.Molecular characterization of thyroid hormone receptors from the leopard gecko,and their differential expression in the skin[J].Zoological Science,2006,23(6):549-556
• [15]Cardone A,Comitato R,Angelini F.Spermatogenesis,epididymis morphology and plasma sex steroid secretion in the male lizard Podarcis sicula exposed to diuron[J].Environmental Research,2008,108(2):214-223
• [16]Suski J G,Salice C,Houpt J T,et al.Dose-related effects following oral exposure of 2,4-dinitrotoluene on the Western fence lizard,Sceloporus occidentalis[J].Environmental Toxicology and Chemistry,2008,27(2):352-359
• [17]戴欣,李伟,李建中,等.蜥蜴生态毒理学研究进展[J].生态毒理学报,2016,11(1):103-110Dai X,Li W,Li J Z,et al.Research advances in lizard ecotoxicology[J].Asian Journal of Ecotoxicology,2016,11(1):103-110(in Chinese).
• [18]常静,杨淞霖,戴欣,等.5种化学品对丽斑麻蜥和日本鹌鹑的毒性研究[J].生态毒理学报,2015,10(6):276-280Chang J,Yang S L,Dai X,et al.Toxicity assessment of five chemicals on Eremias argus and Coturnix japonica[J].Asian Journal of Ecotoxicology,2015,10(6):276-280(in Chinese).
• [19]Weir S M,Suski J G,Salice C J.Ecological risk of anthropogenic pollutants to reptiles:Evaluating assumptions of sensitivity and exposure[J].Environmental Pollution,2010,158(12):3596-3606
• [20]Tully D B,Bao W,Goetz A K,et al.Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides[J].Toxicology and Applied Pharmacology,2006,215(3):260-273
• [21]Peig J,Green A J.New perspectives for estimating body condition from mass/length data:The scaled mass index as an alternative method[J].Oikos,2009,118(12):1883-1891
• [22]Kime D E.‘Classical’and‘non-classical’reproductive steroids in fish[J].Reviews in Fish Biology and Fisheries,1993,3(2):160-180
• [23]Ankley G T,Kahl M D,Jensen K M,et al.Evaluation of the aromatase inhibitor fadrozole in a short-term reproduction assay with the fathead minnow(Pimephales promelas)[J].Toxicological Sciences,2002,67(1):121-130
• [24]Ankley G T,Cavallin J E,Durhan E J,et al.A timecourse analysis of effects of the steroidogenesis inhibitor ketoconazole on components of the hypothalamic-pituitary-gonadal axis of fathead minnows[J].Aquatic Toxicology,2012,114-115(4):88-95
• [25]Ankley G T,Jensen K M,Kahl M D,et al.Ketoconazole in the fathead minnow(Pimephales promelas):Reproductive toxicity and biological compensation[J].Environmental Toxicology and Chemistry,2007,26(6):1214-1223
• [26]Skolness S Y,Blanksma C A,Cavallin J E,et al.Propiconazole inhibits steroidogenesis and reproduction in the fathead minnow(Pimephales promelas)[J].Toxicological Sciences,2013,132(2):284-297
• [27]Muller T A,Kohler H P.Chirality of pollutants-Effects on metabolism and fate[J].Applied Microbiology and Biotechnology,2004,64(3):300-316
• [28]Liu W,Gan J,Lee S,et al.Isomer selectivity in aquatic toxicity and biodegradation of bifenthrin and permethrin[J].Environmental Toxicology and Chemistry,2005,24(8):1861
• [29]Liu W,Gan J J,Qin S.Separation and aquatic toxicity of enantiomers of synthetic pyrethroid insecticides[J].Chirality,2005,17(Suppl):S127-S133
• [30]Vinggaard A M,Hnida C,Breinholt V,et al.Screening of selected pesticides for inhibition of CYP19 aromatase activity in vitro[J].Toxicology in Vitro,2000,14(3):227-234
• [31]He Y,Wiseman S B,Wang N,et al.Transcriptional responses of the brain-gonad-liver axis of fathead minnows exposed to untreated and ozone-treated oil sands process-affected water[J].Environmental Science&Technology,2012,46(17):9701-9708
• [32]Zhang X,Hecker M,Park J W,et al.Time-dependent transcriptional profiles of genes of the hypothalamic-pituitary-gonadal axis in medaka(Oryzias latipes)exposed to fadrozole and 17beta-trenbolone[J].Environmental Toxicology and Chemistry,2008,27(12):2504-2511