李娟;全占军;张春晖;史娜娜;韩煜;王琦;张淇翔;

• 1:中国矿业大学(北京)化学与环境工程学院
• 2:中国环境科学研究院

摘要(Abstract)：

主要从植物根细胞壁积累固定、细胞膜对铜的吸收控制、金属配体的螯合作用、铜在系统液泡的分隔机制及胁迫蛋白的合成5个方面,分别阐述植物对铜分子的耐性机制的研究进展,全面了解了铜在植物中的亚细胞分布、铜在植物根系到地上部分运输过程的转运机制以及植物在铜胁迫下的抗性反应等。并在此基础上提出了存在的问题以及今后研究的重点。

关键词(KeyWords)： 铜;植物;细胞膜;螯合作用;胁迫蛋白

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务专项(2013-YSKY-14);; 云南省环境保护厅生物多样性保护专项“滇池土著水生植物保育区构建示范研究”

作者(Author): 李娟;全占军;张春晖;史娜娜;韩煜;王琦;张淇翔;

Email:

参考文献(References)：

• [1]Pilon M,Abdel-Ghany S E,Cohu C M,et al.Copper cofactor delivery in plant cells[J].Current Opinion in Plant Biology,2006,9(3):256-263
• [2]Gill T,Dogra V,Kumar S,et al.Protein dynamics during seed germination under copper stress in Arabidopsis over-expressing potentilla superoxide dismutase[J].Journal of Plant Research,2012,125(1):165-172
• [3]王小玲,高柱,黄益宗,等.铜胁迫对3种草本植物生长和重金属积累的影响[J].生态毒理学报,2014,9(4):699-706Wang X L,Gao Z,Huang Y Z,et al.Effects of copper stress on three kinds of herbaceous plants growth and heavy metal accumultaion[J].Asian Journal of Ecotoxicology,2014,9(4):699-706(in Chinese)
• [4]Sahi S V,Israr M,Srivastava A K,et al.Accumulation,speciation and cellular localization of copper in Sesbania drummondii[J].Chemosphere,2007,67(11):2257-2266
• [5]Garcia L,Welchen E,Gonzalez D H.Mitochondria and copper homeostasis in plants[J].Mitochondrion,2014,19:269-274
• [6]申鸿,刘于,李晓林,等.丛枝菌根真菌(Glomus caledonium)对铜污染土壤生物修复机理初探[J].植物营养与肥料学报,2005,11(2):199-204Shen H,Liu Y,Li X L,et al.Influence of arbuscular mycorrhizal fungus(Glomus caledonium)on maize seedlings grown in copper contaminated soil[J].Plant Nutrition and Fertilizer Science,2005,11(2):199-204(in Chinese)
• [7]刘小红.九华铜矿重金属污染调查及耐铜植物的筛选、耐性机理研究[D].合肥:安徽农业大学,2005:35-36Liu X H.Heavy metal contamination and copper tolerant plants in the vicinity of Jiuhua copper mine[D].Hefei:Anhui Agricultural University,2005:35-36(in Chinese)
• [8]Konno H,Nakashima S,Katoh K.Metal-tolerant moss Scopelophila cataractae accumulates copper in the cell wall pectin of the protonema[J].Journal of Plant Physiology,2010,167(5):358-364
• [9]Krzesowska M.The cell wall in plant cell response to trace metals:Polysaccharide remodeling and its role in defense strategy[J].Acta Physiologiae Plantarum,2011,33:35-51
• [10]Konno H,Nakato T,Nakashima S,et al.Lygodium japonicum fern accumulates copper in the cell wall pectin[J].Journal of Experimental Botany,2005,56(417):1923-1931
• [11]Liu T,Li F,Zhang X,et al.Tracing intracellular localization and chemical forms of copper in Elsholtzia splendens with cluster analysis[J].Biological Trace Element Research,2014,160(3):418-426
• [12]刘婷婷,彭程,王梦,等.海州香薷根细胞壁对铜的吸附固定机制研究[J].环境科学学报,2014,34(2):514-523Liu T T,Peng C,Wang M,et al.Mechanism of fixation and adsorption of copper on root cell wall of Elsholtzia splendens[J].Acta Scientiae Circumstantiae,2014,34(2):514-523(in Chinese)
• [13]Pelloux J,Rusterucci C,Mellerowicz E J.New insights into pectin methylesterase structure and function[J].Trends in Plant Science,2007,12(6):267-277
• [14]Davis T A,Volesky B,Mucci A.A review of the biochemistry of heavy metal biosorption by brown algae[J].Water Research,2003,37(18):4311-4330
• [15]武贝.海州香薷Cu耐性与积累机制研究[D].杭州:浙江大学,2009:45-49Wu B.Study of mechanisms for Cu tolerance and accu-mulation in Elsholtzia splendens[D].Hangzhou:Zhejiang University,2009:45-49(in Chinese)
• [16]Merdy P,Guillon E,Dumonceau J,et al.Spectroscopic study of copper(II)--wheat straw cell wall residue surface complexes[J].Environmental Science&Technology,2002,36(8):1728-1733
• [17]Liu T,Shen C,Wang Y,et al.New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress[J].PLo S One,2014,9(10):e109573
• [18]Pilon M,Cohu C M,Ravet K,et al.Essential transition metal homeostasis in plants[J].Current Opinion in Plant Biology,2009,12(3):347-357
• [19]Küpper H,Zhao F J,Mcgrath S P.Cellular compartmentation of zinc in leaves of the hyperaccumulator Thlaspi caerulescens[J].Plant Physiology,1999,119:305-311
• [20]Sahi S V,Israr M,Srivastava A K,et al.Accumulation,speciation and cellular localization of copper in Sesbania drummondii[J].Chemosphere,2007,67(11):2257-2266
• [21]Puig S,Lee J,Lau M,et al.Biochemical and genetic analyses of yeast and human high affinity copper transporters suggest a conserved mechanism for copper uptake[J].Journal of Biological Chemistry,2002,277(29):26021-26030
• [22]Sancenon V,Puig S,Mira H,et al.Identification of a copper transporter family in Arabidopsis thaliana[J].Plant Molecular Biology,2003,51(4):577-587
• [23]Jung H I,Gayomba S R,Rutzke M A,et al.COPT6 is a plasma membrane transporter that functions in copper homeostasis in Arabidopsis and is a novel target of SQUAMOSA promoter-binding protein-like 7[J].Journal of Biological Chemistry,2012,287(40):33252-33267
• [24]Antoni G,Nuria A,Ana P,et al.The Arabidopsis COPT6 transport protein functions in copper distribution under copper-deficient conditions[J].Plant and Cell Physiology,2013,54(8):1378-1390
• [25]Penarrubia L,Nuria A,Moreno J,et al.Regulation of copper transport in Arabidopsis thaliana:A biochemical oscillator?[J].Journal of Biological Inorganic Chemistry,2010,15(1):29-36
• [26]Nuria A,Ana P,Puig S,et al.Deregulated copper transport affects Arabidopsis development especially in the absence of environmental cycles[J].Plant Physiology,2010,153(1):170-184
• [27]Kampfenkel K,Kushnir S,Babiychuk E,et al.Molecular characterization of a putative Arabidopsis thaliana copper transporter and its yeast homologue[J].Journal of Biological Chemistry,1995,270(47):28479-28486
• [28]Sancenon V,Puig S,Isabel M,et al.The Arabidopsis copper transporter COPT1 functions in root elongation and pollen development[J].Journal of Biological Chemistry,2004,279(15):15348-15355
• [29]张红晓,张芬琴.铜在植物细胞中的运输和分布[J].洛阳理工学院学报:自然科学版,2011,27(3):1-5Zhang H X,Zhang F Q.Copper delivery and distribution in plant cells[J].Journal of Luoyang Institute of Science and Technology:Natural Science Edition,2011,27(3):1-5(in Chinese)
• [30]Eisses J F,Kaplan J H.The mechanism of copper uptake mediated by human CTR1:A mutational analysis[J].Journal of Biological Chemistry,2005,280(44):37159-37168
• [31]Baxter I,Tchieu J,Sussman M R,et al.Genomic comparison of P-type ATPase ion pumps in Arabidopsis and rice[J].American Society of Plant Biologists,2003,132(2):618-628
• [32]金枫,王翠,林海建,等.植物重金属转运蛋白研究进展[J].应用生态学报,2010,21(7):1875-1882Jin F,Wang C,Lin H J,et al.Heavy metal-transport proteins in plants:A review[J].Chinese Journal of Applied Ecology,2010,21(7):1875-1882(in Chinese)
• [33]Nuria A,Vicente S,Susana R,et al.The Arabidopsis heavy metal P-type ATPase HMA5 interacts with metallochaperones and functions in copper detoxification of roots[J].Plant Journal,2006,45(2):225-236
• [34]Williams L E,Mills R F.P(1B)-ATPases--An ancient family of transition metal pumps with diverse functions in plants[J].Trends in Plant Science,2005,10(10):491-502
• [35]Puig S,Mira H,Dorcey E,et al.Higher plants possess two different types of ATX1-like copper chaperones[J].Biochemical and Biophysical Research Communication,2007,354(2):385-390
• [36]Boutigny S,Sautron E,Finazzi G,et al.HMA1 and PAA1,two chloroplast-envelope PIB-ATPases,play distinct roles in chloroplast copper homeostasis[J].Journal of Experimental Botany,2014,65(6):1529-1540
• [37]Daphne S,Gravot A,Auroy P,et al.HMA1,a new Cu-ATPase of the chloroplast envelope,is essential for growth under adverse light conditions[J].Journal of Biological Chemistry,2006,281(5):2882-2892
• [38]Salah E A,Patricia M,Niyogi K K,et al.Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts[J].Plant Cell,2005,17(4):1233-1251
• [39]Rodriguez F I,Esch J J,Hall A E,et al.A copper cofactor for the ethylene receptor ETR1 from Arabidopsis[J].Science,1999,283(5404):996-998
• [40]Chen Y F,Randlett M D,Findell J L,et al.Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis[J].Journal of Biological Chemistry,2002,277(22):19861-19866
• [41]张玉秀,张媛雅,孙涛,等.植物重金属转运蛋白P1B-ATPase结构和功能研究进展[J].生物工程学报,2010,25(6):715-725Zhang Y X,Zhang Y Y,Sun T,et al.Structure and function of heavy metal transporter P1B-ATPase in plant:A review[J].Chinese Journal of Biotechnology,2010,25(6):715-725(in Chinese)
• [42]Deng F,Yamaji N,Xia J,et al.A member of the heavy metal P-type ATPase Os HMA5 is involved in xylem loading of copper in rice[J].Plant Physiology,2013,163:1353-1362
• [43]Pufahl R A,Singer C P,Peariso K L,et al.Metal ion chaperone function of the soluble Cu(I)receptor Atx1[J].Science,1997,278(5339):853-856
• [44]Himelblau E,Mira H,Lin S J,et al.Identification of a functional homolog of the yeast copper homeostasis gene ATX1 from Arabidopsis[J].Plant Physiology,1998,117(4):1227-1234
• [45]伍盈盈.金属伴侣蛋白研究进展[J].科技信息,2009,33:428-429Wu Y Y.The recent advances in study of metallochaperones[J].Science&Technology Information,2009,33:428-429(in Chinese)
• [46]Mira H,Fernando M,Penarrubia L.Evidence for the plant-specific intercellular transport of the Arabidopsis copper chaperone CCH[J].Plant Journal,2001,25(5):521-528
• [47]Balandin T,Castresana C.At COX17,an Arabidopsis homolog of the yeast copper chaperone COX17[J].Plant Physiology,2002,129(4):1852-1857
• [48]Chu C C,Lee W C,Guo W Y,et al.A copper chaperone for superoxide dismutase that confers three types of copper/zinc superoxide dismutase activity in Arabidopsis[J].Plant Physiology,2005,139(1):425-436
• [49]Lin S J,Pufahl R A,Dancis A,et al.A role for the Saccharomyces cerevisiae ATX1 gene in copper trafficking and iron transport[J].Journal of Biological Chemistry,1997,272(14):9215-9220
• [50]Harrison M D,Jones C E,Solioz M,et al.Intracellular copper routing:The role of copper chaperones[J].Trends in Biochemical Sciences,2000,25(1):29-32
• [51]Woeste K E,Kieber J J.A strong loss-of-function mutation in RAN1 results in constitutive activation of the ethylene response pathway as well as a rosette-lethal phenotype[J].Plant Cell,2000,12(3):443-455
• [52]Salah E A,Burkhead J L,Gogolin K A,et al.At CCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7[J].FEBS Letters,2005,579(11):2307-2312
• [53]Yruela I.Copper in plants:Acquisition,transport and interactions[J].Functional Plant Biology,2009,36(5):1-64
• [54]傅晓萍,豆长明,胡少平,等.有机酸在植物对重金属耐性和解毒机制中的作用[J].植物生态学报,2010,34(11):1354-1358Fu X P,Dou C M,Hu S P,et al.A review of progress in roles of organic acids on heavy metal resistance and detoxification in plants[J].Chinese Journal of Plant Ecology,2010,34(11):1354-1358(in Chinese)
• [55]Murphy A S,Eisinger W R,Shaff J E,et al.Early copper-induced leakage of K(+)from Arabidopsis seedlings is mediated by ion channels and coupled to citrate efflux[J].Plant Physiology,1999,121(4):1375-1382
• [56]Bernal M,Sánchez-Testillano P,Risueo M,et al.Excess copper induces structural changes in cultured photosynthetic soybean cells[J].Functional Plant Biology,2006,33(11):1001-1012
• [57]White M C,Baker F D,Chaney R L,et al.Metal complexation in xylem fluid:II.Theoretical equilibrium model and computational computer program[J].Plant Physiology,1981,67(2):301-310
• [58]刘红云.水稻(Oryza sativa L.)耐铜品种的筛选及其耐性机理研究[D].南京:南京农业大学,2007:86-89Liu H Y.Identification of rice varieties with high tolerance or sensitivity to copper and characterization of the mechanisms of tolerance[D].Nanjing:Nanjing Agricultural University,2007:86-89(in Chinese)
• [59]张玉秀,柴团耀,Burkard G.植物耐重金属机理研究进展[J].植物学报,1999,41(5):453-457Zhang Y X,Chai T Y,Burkard G.Research advances on the mechanisms of heavy metal tolerance in plants[J].Acta Botanica Sinica,1999,41(5):453-457(in Chinese)
• [60]Guo W J,Meetam M,Goldsbrough P B.Examining the specific contributions of individual Arabidopsis metallothioneins to copper distribution and metal tolerance[J].Plant Physiology,2008,146(4):1697-1706
• [61]Zhang H,Lian C,Shen Z.Proteomic identification of small,copper-responsive proteins in germinating embryos of Oryza sativa[J].Annals of Botany,2009,103(6):923-930
• [62]Thomas J C,Davies E C,Malick F K,et al.Yeast metallothionein in transgenic tobacco promotes copper uptake from contaminated soils[J].Biotechnology Progress,2003,19(2):273-280
• [63]Liu J,Shi X,Qian M,et al.Copper-induced hydrogen peroxide upregulation of a metallothionein gene,OsMT2c,from Oryza sativa L.confers copper tolerance in Arabidopsis thaliana[J].Journal of Hazardous Materials,2015,294:99-108
• [64]Rauser W E.Structure and function of metal chelators produced by plants:The case for organic acids,amino acids,phytin,and metallothioneins[J].Cell Biochemistry and Biophysics,1999,31(1):19-48
• [65]Cobbett C,Goldsbrough P.Phytochelatins and metallothioneins:Roles in heavy metal detoxification and homeostasis[J].Annual Review of Plant Biology,2002,53:159-182
• [66]Lee S,Kang B S.Phytochelatin is not a primary factor in determining copper tolerance[J].Journal of Plant Biology,2005,48(1):32-38
• [67]Boojar M M A,Tavakoli Z.Role of antioxidant enzyme response and phytochelatins in tolerance strategies of Alhagi camelorum Fisch growing on copper mine[J].Acta Botanica Croatica,2010,69(1):107-121
• [68]Liu D,Kottke I.Subcellular localization of copper in the root cells of Allium sativum by electron energy loss spectroscopy(EELS)[J].Bioresource Technology,2004,94(2):153-158
• [69]Elbaz B,Noa S,Ora D,et al.High expression in leaves of the zinc hyperaccumulator Arabidopsis halleri of Ah MHX,a homolog of an Arabidopsis thaliana vacuolar metal/proton exchanger[J].Plant Cell and Environment,2006,29(6):1179-1190
• [70]Burleigh S H,Kristensen B K,Bechmann I E.A plasma membrane zinc transporter from Medicago truncatula is up-regulated in roots by Zn fertilization,yet down-regulated by arbuscular mycorrhizal colonization[J].Plant Molecular Biology,2003,52(5):1077-1088
• [71]Wu L,Thurman D A,Bradshaw A D.The uptake of copper and its effect upon respiratory processes of roots of copper-tolerant and non-tolerant clones of Agrostis stolonifera[J].New Phytologist,1975,75:225-229
• [72]谭九洲,黄迎波.植物重金属耐受分子机理的研究进展[J].安徽农业科学,2014,42(35):12782-12785Tan J Z,Huang Y B.Research progress of the molecular mechanism of heavy metal tolerance of plants[J].Journal of Anhui Agricultural Sciences,2014,42(35):12782-12785(in Chinese)
• [73]张黛静,王多多,董文,等.铜胁迫下小麦幼根转录组学及蛋白质组学研究[J].河南农业科学,2015,44(4):31-35Zhang D J,Wang D D,Dong W,et al.Transcriptomics and proteomics analysis in root of wheat under copper stress[J].Journal of Henan Agricultural Sciences,2015,44(4):31-35(in Chinese)
• [74]Kung C C,Huang W N,Huang Y C,et al.Proteomic survey of copper-binding proteins in Arabidopsis roots by immobilized metal affinity chromatography and mass spectrometry[J].Proteomics,2006,6(9):2746-2758
• [75]Chen C,Song Y,Zhuang K,et al.Proteomic analysis of copper-binding proteins in excess copper-stressed roots of two rice(Oryza sativa L.)varieties with different Cu tolerances[J].PLo S One,2015,10(4):e0125367
• [76]Smith A P,Deridder B P,Guo W J,et al.Proteomic analysis of Arabidopsis glutathione S-transferases from benoxacor-and copper-treated seedlings[J].Journal of Biological Chemistry,2004,279(25):26098-26104
• [77]Li G,Peng X,Xuan H,et al.Proteomic analysis of leaves and roots of common wheat(Triticum aestivum L.)under copper-stress conditions[J].Journal of Proteome Research,2013,12(11):4846-4861