急性毒性藻红外测试中敏感藻的确定方法Research on the Method of Determining Sensitive Algae in Measuring Algae Infrared Radiation to Respond to Acute Toxicity
郭蔚华;丁燕燕;张智;王翔;苏海燕;
摘要(Abstract):
敏感藻是指在急性毒性藻红外测试中对毒物响应的温差大、时间快、种类多、剂量低的特殊藻类.自然藻中是否存在敏感藻以及如何确定,是建立藻红外测试法的关键.为此,开展了敏感藻确定方法的探索性研究.试验用8种藻,重金属、有机毒物、农药各10种进行测试分析,结果表明:短线脆杆藻(Fragilaria brevistriata)对重金属、羊角月牙藻(Selenastrum capricornutum)对有机毒物、纤细裸藻(Euglena gracilis)对除草剂和杀虫剂、水华鱼腥藻(Anabaena flos-aquae)对杀菌剂的平均绝对温差分别为0.21℃、0.27℃、0.19~0.20℃、0.20℃,分别高出各自的总均温差0.07℃、0.15℃、0.07℃、0.08℃;4种藻的平均响应时间1.5~7.0min,较好响应药品的响应率≥80%;发光细菌对部分药品的灵敏度在0.07~50.0mg·L-1,而4种藻对相同药品的灵敏度在0.01~7.0mg·L-1.根据敏感藻定义和试验结果,确定短线脆杆藻、羊角月牙藻、纤细裸藻、水华鱼腥藻分别为重金属、有机毒物、除草剂和杀虫剂、杀菌剂的敏感藻.
关键词(KeyWords): 敏感藻确定;藻红外测试;重金属;有机毒物;农药
基金项目(Foundation): 重庆市自然科学基金项目(CSTC,No.2005BB7254);; 重庆市科技攻关计划项目(CSTC,No.2006AB7054)
作者(Author): 郭蔚华;丁燕燕;张智;王翔;苏海燕;
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