李爽;盛连喜;姜海波;

• 1:北华大学林学院环境科学系
• 2:东北师范大学环境科学系

摘要(Abstract)：

虽然水生生物大多是变温动物并且温度对化合物的毒性有非常重要的作用,但生态毒理学实验一般在实验室标准温度(20℃)或者生物最适温度下进行。我们调查了适应温度(20℃、24℃和28℃)对镉暴露(0,20和40μg·L~(-1))中大型溞生理和亚细胞水平的作用,实验终点包括镉的急性毒性(48 h-EC50)、大型溞的21 d存活率、21 d累积繁殖数(21-d CR)、第一次生育时间(time to first brood,TFB)、能量储存、电子转移活性(electron transport system activity,ETS)、热应激蛋白hsp70和金属硫蛋白(MT)。结果显示:适应温度升高时大型溞的镉耐性降低,20℃、24℃和28℃下的48 h-EC50分别为(40.2±9.9)μg·L~(-1)、(15.6±2.7)μg·L~(-1)和(10.7±1.9)μg·L~(-1);28℃下,大型溞21 d存活率分别比对照(20℃,0μg·L~(-1)镉暴露)降低23%(20μg·L~(-1)镉暴露)和87%(40μg·L~(-1)镉暴露)并且大型溞的21 d累积繁殖数(21-d CR)显著降低。大型溞的能量储存随适应温度的升高而降低,24℃和28℃时分别降低了46%和62%(0μg·L~(-1)镉暴露),48%和60%(20μg·L~(-1)镉暴露)、80%和91%(40μg·L~(-1)镉暴露)。而镉浓度只有达到40μg·L~(-1)时,对大型溞的能量储存有明显影响。适应温度和镉暴露对电子转移系统活性(ETS)没有显著影响。28℃的适应温度和40μg·L~(-1)的镉暴露都能诱导大型溞热应激蛋白hsp70和金属硫蛋白(MT)显著上升,但适应温度(20℃~28℃)和镉暴露(0~40μg·L~(-1))的结合对hsp70先诱导后抑制,MT则一直被诱导上升。总之,生物在不同温度下对毒物的反应存在差异,将不同适应温度下的毒性数据整合到生态危险性评价能够保证生物获得更充分的保护;此外,hsp70和MT的变化是环境压力的综合反应,作为某种污染物的生物标志物时需要综合考虑环境因素。

关键词(KeyWords)： 镉;温度;大型溞;21 d存活率;能量储存;Hsp70;MT

Abstract:

Keywords:

基金项目(Foundation): 北华大学博士科研基金;; 吉林省教育厅“十三五”科学技术项目(JJKH20170039KJ)

作者(Author): 李爽;盛连喜;姜海波;

Email:

DOI:

参考文献(References)：

• [1]Vergauwen L,Knapen D,Hagenaars A,et al.Hypothermal and hyperthermal acclimation differentially modulate cadmium accumulation and toxicity in the zebrafish[J].Chemosphere,2013,91:521-529
• [2]Vergauwen L,Benoot D,Blust R,et al.Long-term warm or cold acclimation elicits a specific transcriptional response and affects energy metabolism in zebrafish[J].Comparative Biochemistry and Physiology Part A:Molecular and Integrative Physiology,2010,157:149-157
• [3]Fisher S W,Hwang H,Atanasoff M,et al.Lethal body residues for pentachlorophenol in zebra mussels(Dreissena polymorpha)under varying conditions of temperature and p H[J].Ecotoxicology and Environment Safety,1999,43:274-283
• [4]Heugens E H W,Jager T,Creyghton R,et al.Temperature-dependent effects of cadmium on Daphnia magna:accumulation versus sensitivity[J].Environmental Science and Technology,2003,37:2145-2151
• [5]Donker M H,Abdel-Lateif H M,Khalil M A,et al.Temperature,physiological time,and zinc toxicity in the isopod Porcellio scaber[J].Environmental Toxicology and Chemistry,1998,17(8):1558-1563
• [6]Heugens E H W,Hendriks A J,Dekker T,et al.A review of the effects of multiple stressors on aquatic organisms and analysis of uncertainty factors for use in risk assessment[J].Critical Reviews in Toxicology,2001,31(3):247-284
• [7]Sokolova I M,Lannig G.Interactive effects of metal pollution and temperature on metabolism in aquatic ectotherms:Implications of global climate change[J].Climate Research,2008,37:181-201
• [8]Pinot F,Kreps S E,Bachelet M,et al.Cadmium in the environment:Sources,mechanisms of biotoxicity,and biomarkers[J].Reviews Environmental Health,2000,15:299-323
• [9]Vergauwen L,Hagenaars A,Blust R,et al.Temperature dependence of long-term cadmium toxicity in the zebrafish is not explained by liver oxidative stress:Evidence from transcript expression to physiology[J].Aquatic Toxicology,2013,126:52-62
• [10]Organization for Economic Cooperation and Development.Report of the final ring test of the Daphnia magna reproduction test.OECD Series on Testing and Assessment 6[S].Paris:OECD,1997
• [11]International Organization for Standardization.ISO 8692.Water quality—Freshwater algal growth inhibition test with Scenedesmus subspicatus and Selenastrum capricornutum[S].Geneva:ISO,1989
• [12]Organization for Economic Cooperation and Development.Guideline 202.Daphnia sp,Acute Immobilisation Test[S].Paris:OECD,2004
• [13]De Coen W M,Janssen C R.The use of biomarkers in Daphnia magna toxicity testing.IV.Cellular energy allocation:A new methodology to assess the energy budget of toxicant-stressed Daphnia populations[J].Journal of Aquatic Ecosystem Stress Recovery,1997,6:4-55
• [14]Haap T,Triebskorn R,K9hler H R.Acute effects of diclofenac and DMSO to Daphnia magna:Immobilisation and hsp70 induction[J].Chemosphere,2008,73:353-359
• [15]Haap T,K9hler H R.Cadmium tolerance in seven Daphnia magna clones is associated with reduced hsp70 baseline levels and induction[J].Aquatic Toxicology,2009,94:131-137
• [16]Scheuhammer A M,Cherian M G.Quantification of metallothioneins by a silver-saturation method[J].Toxicology and Applied Pharmacology,1986,3:417-425
• [17]Stuhlbacher A,Bradley M C,Naylor C,et al.Variation in the development of cadmium resistance in Daphnia magna straus:Effect of temperature,nutrition,age and genotype[J].Environmental Pollution,1993,80:153-158
• [18]Work K A,Gophen M.Factors which affect the abundance of an invasive cladoceran,Daphnia lumholtzi,in U.S.reservoirs[J].Freshwater Biology,1999,42:1-10
• [19]Sibly R M,Calow P.A life-cycle theory of responses to stress[J].Biological Journal of the Linnean Society,1989,37:101-116
• [20]P9rtner H O.Climate variations and the physiological basis of temperature dependent biogeography:Systemic to molecular hierarchy of thermal tolerance in animals[J].Comparative Biochemistry and Physiology A:Physiology,2002,132:739-761
• [21]Soetaert A,Vandenbrouck T,Van D V K,et al.Molecular responses during cadmium-induced stress in Daphniamagna:Integration of differential gene expression with higher-level effects[J].Aquatic Toxicology,2007,83:212-222
• [22]Muyssen B T A,Janssen C R.Multi-generation cadmium acclimation and tolerance in Daphnia magna[J].Environmental Pollution,2004,130:309-316
• [23]Cherkasov A S,Biswas P K,Ridings D M,et al.Effects of acclimation temperature and cadmium exposure on cellular energy budgets in marine mollusc Crassostrea virginica:Linking cellular and mitochondrial responses[J].Journal of Experimental Biology,2006,209:1274-1284
• [24]Lannig G,Cherkasov A S,P9rtner H O,et al.Cadmiumdependent oxygen limitation affects temperature tolerance in eastern oysters(Crassostrea virginica Gmelin)[J].American Journal of Physiology,2008,294:1338-1346
• [25]Simˇciˇc T,Brancelj A.Electron transport system(ETS)activity and respiration rate in five Daphnia species at different temperatures[J].Hydrobiologia,1997,360:117-125
• [26]Yampolsky L Y,Scheiner S M.Why larger offspring at lower temperatures?A demographic approach[J].American Naturalist,1996,147:86-100
• [27]Heugens E H W,Tokkie L T B,Kraak M H S,et al.Population growth of Daphnia magna under multiple stress conditions:Joint effects of temperature,food,and cadmium[J].Environmental Toxicology and Chemistry,2006,25:1399-1407
• [28]At-Assa S,Pandard P,Magaud H,et al.Evaluation of an in vitro hsp70 induction test for toxicity assessment of complex mixtures:Comparison with chemical analyses and ecotoxicity tests[J].Ecotoxicology and Environment Safety,2003,54:92-104
• [29]Li S,Xu J B,Sheng L X.The trans-generation effect during pulsed cadmium exposure:Tolerance and induction of hsp70[J].Ecotoxicology and Environment Safety,2014,107:300-305
• [30]Osovitz C J,Hofmann G E.Thermal history-dependent expression of the hsp70 gene in purple sea urchins:Biogeographic patterns and the effect of temperature acclimation[J].Journal of Experimental Marine Biological Ecology,2005,327(2):134-143
• [31]Deane E E,Woo N Y S.Cloning and characterization of the hsp70 multigene family from silver sea bream:Modulated gene expression between warm and cold temperature acclimation[J].Biochemical and Biophysical Research Communications,2005,330(3):776-783
• [32]Buckley B A,Owen M E,Hofmann G E.Adjusting the thermostat:The threshold induction temperature for the heat-shock response in intertidal mussels(genus Mytilus)changes as a function of thermal history[J].Journal of Experimental Biology,2001,204:3571-3579
• [33]Hamdoun A M,Cheney D P,Cherr G N.Phenotypic plasticity of HSP70 and HSP70 gene expression in the pacific oyster(Crassostrea gigas):Implications for thermal limits and induction of thermal tolerance[J].The Biological Bulletin,2003,205:160-169
• [34]Fangue N A,Hofmeister M,Schulte P M.Intraspecic variation in thermal tolerance and heat shock protein gene expression in common killifish,Fundulus heteroclitus[J].Journal of Experimental Biology,2006,209:2859-2872
• [35]Tomanek L.Variation in the heat shock response and its implication for predicting the effect of global climate change on species'biogeographical distribution ranges and metabolic costs[J].Journal of Experimental Biology,2010,213:971-979
• [36]Hofmann G E.Patterns of hsp gene expression in ectothermicmarine organisms on small to large biogeographic scales[J].Integrative Comparative Biology,2005,45:247-255
• [37]Poynton H C,Varshavsky J R,Chang B.Daphnia magna ecotoxicogenomics provides mechanistic insights into metal toxicity[J].Environmental Science and Technology,2007,41(3):1044-1050
• [38]Connon R,Hooper H L,Sibly R M.Linking molecular and population stress responses in Daphnia magna exposed to cadmium[J].Environmental Science and Technology,2008,42(6):2181-2188
• [39]Poynton H C,Taylor N S,Hicks J,et al.Metabolomics of microliter hemolymph samples enables an improved understanding of the combined metabolic and transcriptional responses of Daphnia magna to cadmium[J].Environmental Science and Technology,2011,45(8):3710-3717
• [40]Bodar C W M,Van Donselaar E G,Herwig H J.Cytopathological investigations of digestive tract and storage cells in Daphnia magna exposed to cadmium and tributyltin[J].Aquatic Toxicology,1990,17(4):325-337
• [41]Klaassen C D,Liu J,Choudhuri S.Metallothionein:An intracellular protein to protect against cadmium toxicity[J].Annual Review of Pharmacology and Toxicology,1999,39:267-294
• [42]Amiard J C,Amiard-Triquet C,Barka S.Metallothioneins in aquatic invertebrates:Their role in metal detoxification and their use as biomarkers[J].Aquatic Toxicology,2006,76:160-202
• [43]Olsson P E,Larsson A,Haux C.Influence of seasonal changes in water temperature on cadmium inducibility ofhepatic and renal metallothionein in rainbow trout[J].Marine Environment Research,1996,42:41-44
• [44]Guinot D,Ure1a R,Pastor A,et al.Long-term effect of temperature on bioaccumulation of dietary metals and metallothionein induction in Sparus aurata[J].Chemosphere,2012,87(11):1215-1221
• [45]Andrews G K.Regulation of metallothionein gene expression by oxidative stress and metal ions[J].Biochemical Pharmacology,2000,59:95-104
• [46]Saydam N,Steiner F,Georgiev O.Heat and heavy metal stress synergize to mediate transcriptional hyper-activation by metal-responsive transcription factor MTF-1[J].Journal of Biological Chemistry,2003,278:31879-31883
• [47]Tamai K T,Liu X,Silar P.Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways[J].Molecular and Cellular Biochemistry,1994,14:8155-8165
• [48]Liu X D,Thiele D J.Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription[J].Genes and Development,1996,10:592-603
• [49]Abele D,Philipp E,Gonzalez P.Marine invertebrate mitochondria and oxidative stress[J].Frontiers in Bioscience,2007,12:933-946
• [50]Abele D,Heise K,P9rtner H O.Temperature-dependence of mitochondrial function and production of reactive oxygen species in the inter-tidal mud clam Mya arenaria[J].Journal of Experimental Biology,2002,205:1831-1841