应用流式细胞分析技术研究微纳尺度SiO_2对雄性大鼠睾丸生精细胞的毒性作用Toxic Effects of Micro-nano-scale SiO_2 on Male Rat Spermatogenic Cells Assessed by Flow Cytometry
林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;
摘要(Abstract):
为了进一步研究纳米与微米尺度SiO2对雄性大鼠的生殖毒性作用,选择不同剂量的纳米SiO2(20~40nm)与微米SiO2(1~10μm),采用气管滴注方式对雄性Wistar大鼠分组染毒.于染毒5周后处死大鼠,应用流式细胞技术对睾丸生精细胞进行分析.结果表明:1)高、低剂量纳米SiO2组及高剂量微米SiO2组细胞凋亡率均显著高于对照组;2)与对照组相比,高、低剂量纳米SiO2组及高剂量微米SiO2组1C细胞显著减少,4C细胞显著增加;3)与对照组相比,高剂量纳米SiO2组和高剂量微米SiO2组G0/G1期细胞比例显著降低,高剂量纳米SiO2组G2/M期细胞比例显著增加.结果提示纳米SiO2能够阻滞细胞周期进程,诱导生精细胞凋亡;与微米SiO2相比,纳米SiO2对大鼠睾丸生精细胞的损伤有更严重的趋势.
关键词(KeyWords): 纳米SiO_2;微米SiO_2;雄性大鼠;流式细胞技术;睾丸;毒性
基金项目(Foundation): 国家自然科学基金项目(No.90406024);; 国家高技术研究发展规划(863)项目(No.2006AA03Z330)
作者(Author): 林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;
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