林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;

• 1:军事医学科学院卫生学环境医学研究所
• 2:军事医学科学院卫生学环境医学研究所
• 3:国家纳米科学中心军事医学科学院纳米药理毒理协作实验室
• 4:军事医学科学院卫生学环境医学研究所

摘要(Abstract)：

为了进一步研究纳米与微米尺度SiO2对雄性大鼠的生殖毒性作用,选择不同剂量的纳米SiO2(20～40nm)与微米SiO2(1～10μm),采用气管滴注方式对雄性Wistar大鼠分组染毒.于染毒5周后处死大鼠,应用流式细胞技术对睾丸生精细胞进行分析.结果表明:1)高、低剂量纳米SiO2组及高剂量微米SiO2组细胞凋亡率均显著高于对照组;2)与对照组相比,高、低剂量纳米SiO2组及高剂量微米SiO2组1C细胞显著减少,4C细胞显著增加;3)与对照组相比,高剂量纳米SiO2组和高剂量微米SiO2组G0/G1期细胞比例显著降低,高剂量纳米SiO2组G2/M期细胞比例显著增加.结果提示纳米SiO2能够阻滞细胞周期进程,诱导生精细胞凋亡;与微米SiO2相比,纳米SiO2对大鼠睾丸生精细胞的损伤有更严重的趋势.

关键词(KeyWords)： 纳米SiO_2;微米SiO_2;雄性大鼠;流式细胞技术;睾丸;毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.90406024);; 国家高技术研究发展规划(863)项目(No.2006AA03Z330)

作者(Author): 林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;

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DOI:

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