姜丽思;张倩茹;王建美;李斯雯;宋婕;崔伟娜;刘宛;

• 1:中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室
• 2:中国科学院大学
• 3:辽宁大学环境学院
• 4:上海应用技术学院

摘要(Abstract)：

由于佳乐麝香(HHCB)被广泛应用于日用化工产品中,被持续不断地释放到环境中,所产生的生态风险已引起越来越多的重视。为探究HHCB的生态毒性效应,在水培条件下考察了不同浓度HHCB对萝卜的表观生长指标(发芽率、根伸长抑制率、芽伸长抑制率)和基于随机引物扩增多态性(RAPD)图谱的根尖DNA损伤状况。研究结果显示:低剂量(≤25 mg·L~(-1))胁迫对萝卜发芽无显著影响(P>0.05);高剂量(≥50 mg·L~(-1))胁迫可以显著抑制萝卜发芽率(P<0.05)。萝卜的根长和芽长抑制率随HHCB浓度增加而呈上升趋势,且根伸长对HHCB胁迫较芽伸长更敏感,更适宜指示HHCB对植物的生态毒性效应。萝卜根尖基因组DNA的RAPD分析结果表明:大于或等于5 mg·L~(-1)的HHCB即可明显导致萝卜根尖基因组DNA损伤,且随着HHCB浓度的升高,根尖基因组DNA含量呈线性降低,DNA多态率增加,基因组模板稳定性(GTS)减小,遗传相似性变远。这表明较低剂量的HHCB胁迫就能够导致萝卜根尖基因组DNA损伤,且随浓度升高而损伤严重。因此,利用RAPD技术获得的萝卜DNA多态性变化可作为检测HHCB遗传毒性效应的敏感生物标记物,为化学品污染生态毒理早期诊断提供科学依据。

关键词(KeyWords)： 佳乐麝香;萝卜;生态毒性;DNA损伤;RAPD

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31470552,31670516);; “十二五”国家科技支撑计划项目(2015BAD05B03)

作者(Author): 姜丽思;张倩茹;王建美;李斯雯;宋婕;崔伟娜;刘宛;

Email:

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