多氯联苯对孔雀鱼卵黄蛋白原的诱导及检测Induction and Determination of Vitellogenin in Male Guppies(Poecilia reticulata)after Exposed to Polychlorinated Biphenyls
詹翠琼;黄絮洁;方展强;马广智;姚静;
摘要(Abstract)：
为探讨利用孔雀鱼(Poecilia reticulata)卵黄蛋白原(Vitellogenin,Vtg)作为生物标志物检测环境雌激素的可行性,采用浸浴法分别使用17β-雌二醇(E2)和多氯联苯(PCBs)对雄性孔雀鱼成鱼进行染毒,30d后测定其性腺系数(GSI)和肝指数(LSI),并将鱼体整体匀浆进行常规聚丙烯酰胺凝胶电泳(Native-PAGE)及磷、脂和糖蛋白特异染色分析.结果表明,PCBs对雄性孔雀鱼具有雌激素效应,和E2均可以诱导雄性孔雀鱼体内产生Vtg,但诱导组雄鱼GSI及LSI与对照组比较均无显著性差异(p>0.05).Native-PAGE及磷、脂、糖蛋白特异性分析表明,孔雀鱼Vtg是一种富含磷、脂、糖的蛋白,具有VtgⅠ、VtgⅡ和VtgⅢ等3种形式,其分子量分别为642kDa、541kDa和441kDa.雄性孔雀鱼Vtg可作为环境雌激素监测的有效生物标记物.
关键词(KeyWords)： 17β-雌二醇;多氯联苯;卵黄蛋白原;常规聚丙烯酰胺凝胶电泳;孔雀鱼
基金项目(Foundation): 广东省科技计划项目(No.2004B40101015;No.2006B36601008); 华南师范大学2004-2005年度学生课外科研项目(No.67)
作者(Author): 詹翠琼;黄絮洁;方展强;马广智;姚静;
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DOI:
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