两种典型持久性有机污染物对草履虫急性毒性初探(英文)Initial Study on Acute Toxicity of Two Typical Persistent Organic Pollutants(POPs) against Paramecium caudatum
周海龙;李国锋;黄仁良;崔嵩;刁晓平;
摘要(Abstract):
滴滴涕和苯并芘这两种典型持久性有机污染物在环境中已广泛分布,因此,本研究利用单细胞真核模式生物-草履虫来研究其急性毒性效应,结果发现其毒性效应存在显著的剂量效应关系。DDT和BaP的半数致死浓度分别为126.012mg.L-1和180.167mg.L-1,且这两种污染物的浓度和概率间存在很好的线性关系。不同浓度的DDT和BaP对草履虫进行毒性作用时,草履虫呈现出不同的形态;比较而言,DDT的毒性作用更大。由于草履虫对这两种毒性物质作用的敏感性,因此,草履虫可作为一种敏感指示生物来评估POPs的长期危害。本研究为水污染的减排和生境的保护提供了一种新途径。最后,就这两种典型POPs对草履虫的毒性机理进行了讨论。
关键词(KeyWords): 草履虫;滴滴涕;苯并芘;半数致死浓度;毒性机理
基金项目(Foundation): The Natural Science Foundation of Hainan Province(309010);; the Science and Research Programme of Education Department of Hainan Province(Hj2009-21);; Program of Key Science and Technology in Haikou City(2009-47)
作者(Author): 周海龙;李国锋;黄仁良;崔嵩;刁晓平;
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参考文献(References):
- Catallo W,Hoover D,Vargas D.1994.Effects of an environ-mental toxicant on ultrastructureinParameciumcaudatumandthe protective effect of dissolved calcium[J].Aquatic Toxicol-ogy,29(3-4):291-303
- Finney D.1971.Probit analysis[M].Cambridge:CambridgeUniversity Press
- Garad U,Desai S N,Desai P V.2007.Toxic effects of mono-crotophos onParameciumcaudatum[J].African Journal ofBiotechnology,6(19):2245-2250
- Howard J,Fazio T.1980.Analytical methodology and repor-ted findings of polycyclic aromatic hydrocarbons in foods[J].Journal-Association of Official Analytical Chemists,63(5):1077-1104
- Iwadate Y,Nakaoka Y.2008.Calciumregulates independent-ly ciliary beat and cell contraction inParameciumcells[J].Cell Calcium,44(2):169-179
- Iwata H,Tanabe S,Sakal N,et al.1993.Distribution of per-sistent organochlorines in the oceanic air and surface seawaterand the role of ocean on their global transport and fate[J].Environmental Science&Technology,27(6):1080-1098
- Jones K C,De Voogt P.1999.Persistent organic pollutants(POPs):State of the science[J].Environmental Pollution,100(1-3):209-221
- Juchelka C M,Snell T W.1995.Rapid toxicity toxicity as-sessment using ingestion rate of cladocerans and ciliates[J].Archives of Environmental Contamination and Toxicology,28(2):508-512
- Kazerouni N,Sinha R,Hsu C H,et al.2001.Analysis of200food items for benzo[a]pyrene and esti mation of its intakein anepidemiologic study[J].Food and Chemical Toxicology,39(5):423-436
- Komala Z.1992.Toxicity of fastac10-EC,a pyrethroidinsecticide,toParameciumpri maureliaandTubifexsp.[J].Fo-lia Biologica,40(3-4):109-112
- Koumanova B.2007.Distribution of POPs in Aquatic Ecosys-tems and Processes for their Removal[M].The Fate of Per-sistent Organic Pollutants in the Environment,Germany:Springer,239-249
- Lilius H,Isomaa B,Hol mstr MT.1994.Acomparison of thetoxicity of50reference chemicals to freshly isolated rainbowtrout hepatocytes andDaphnia magna[J].Aquatic toxicolo-gy,30(1):47-60
- Longnecker M P,Klebanoff M A,Zhou H B,et al.2001.Association between maternal serumconcentration of the DDTmetabolite DDE and preterm and small-for-gestational-age ba-bies at birth[J].The Lancet,358(9276):110-114
- Miyoshi N,Kawano T,Tanaka M,et al.2003.Use of para-mecium species in bioassays for environmental risk manage-ment:Determination of IC50values for water pollutants[J].Journal of Health Science,49(6):429-435
- Rao J,Arepalli S,Gunda V,et al.2008.Assessment of cy-toskeletal damage inParameciumcaudatum:An early warn-ing system for apoptotic studies[J].Pesticide Biochemistryand Physiology,91(2):75-80
- Rao J,Gunda V,Srikanth K,et al.2007.Acute toxicity bio-assay usingParameciumcaudatum,a key member to study theeffects of monocrotophos on swi mming behaviour,morphologyand reproduction[J].Toxicological and Environmental Chem-istry,89(1-4):307-317
- Rouabhi R,Berrebbah H,Djebar MR.2006.Toxicity evalua-tion of flucycloxuron and diflubenzuron on the cellular model,Parameciumsp[J].African Journal of Biotechnology,5(1):45-48
- Samara F.2007.Persistent organic pollutants(POPs)in theenvironment and their endocrine disrupting effects onfish[D].Buffalo:State University of New York
- Shiny K,Remani K,Nirmala E,et al.2005.Biotreat ment ofwastewater using aquatic invertebrates,Daphnia magnaandParameciumcaudatum[J].Bioresource Technology,96(1):55-58
- Smoke I,Smoking I.2004.IARC Monographs on the Evalua-tion of Carcinogenic Risks to Humans[M].Lyon,France:IARC Press
- Turusov V,Rakitsky V,Tomatis L.2002.Dichlorodiphenyl-trichloroethane(DDT):Ubiquity,persistence,and risks[J].Environmental Health Perspectives,110(2):125-128
- Van Wendel de Joode B,Wesseling C,Kromhout H,et al.2001.Chronic nervous-systemeffects of long-termoccupation-al exposure to DDT[J].The Lancet,357(9261):1014-1016
- Venkateswara Rao J,Srikanth K,Arepalli S,et al.2006.Toxic effects of acephate onParameciumcaudatumwith spe-cial emphasis on morphology,behaviour,and generation ti me[J].Pesticide Biochemistry and Physiology,86(3):131-137
- Wang T,Zhang Z,Huang J,et al.2007.Occurrence of dis-solved polychlorinated biphenyls and organic chlorinated pesti-cides in the surface water of haihe river and Bohai Bay,China[J].Environmental Science&Technology,28(4):730-735
- Wania F,Mackay D.1996.Tracking the distribution of per-sistent organic pollutants[J].Environmental Science&Tech-nology,30(9):A390-A396
- Zhou H,Qu Y,Wu H,et al.2010.Molecular phylogeniesand evolutionary behavior of AhR(aryl hydrocarbon receptor)pathway genes in aquatic ani mals:I mplications for the toxicol-ogy mechanism of some persistent organic pollutants(POPs)[J].Chemosphere,78(2):193-205