Cu~(2+)、Zn~(2+)、Cd~(2+)、Cr~(6+)和Mn~(2+)对萼花臂尾轮虫联合急性毒性研究Combined Acute Toxicities of Cu~(2+),Zn~(2+),Cd~(2+),Cr~(6+),and Mn~(2+) to Freshwater Rotifer Brachionus calyciflorus Pallas
徐晓平;席贻龙;黄林;
摘要(Abstract)：
为探究重金属复合污染对轮虫的毒性影响,以萼花臂尾轮虫为受试动物,选择Cu~(2+)、Zn~(2+)、Cd~(2+)、Cr6+和Mn~(2+)等5种重金属,采用水生毒理联合效应相加指数法开展了其24 h联合急性毒性作用的评价研究。结果显示,Cu~(2+)、Zn~(2+)、Cd~(2+)、Cr6+和Mn~(2+)等5种重金属对萼花臂尾轮虫24 h半数致死浓度分别为:0.00616 mg·L~(-1),12.62 mg·L-1,2.89 mg·L-1,17.29 mg·L-1和67.32 mg·L-1。联合急性毒性实验结果显示,等毒性配比的Cu~(2+)-Cr6+(0.00385-10.806 mg·L-1)和等浓度配比的Cu~(2+)-Zn~(2+)(0.0199-0.0199 mg·L-1)、Cu~(2+)-Cd~(2+)(0.0181-0.0181 mg·L-1)、Cu~(2+)-Cr6+(0.0118-0.0118 mg·L~(-1))、Zn~(2+)-Cd~(2+)(3.475-3.475 mg·L-1)二元联合测试液的作用结果显示为拮抗效应,其余二元联合测试液的作用结果则均显示是协同效应。等毒性配比的Cu~(2+)-Cr~(6+)-Mn~(2+)(0.00210-5.902-22.981 mg·L-1)和等浓度配比的Cu~(2+)-Cd~(2+)-Mn~(2+)(0.00727-0.00727-0.00727 mg·L-1)三元联合测试液的作用结果显示为拮抗效应,其余三元联合测试液的作用结果则均显示是协同效应。等浓度配比的Cu~(2+)-Zn~(2+)-Cd~(2+)-Cr6+(0.00907-0.00907-0.00907-0.00907 mg·L-1)、Cu~(2+)-Zn~(2+)-Cd~(2+)-Mn~(2+)(0.00898-0.00898-0.00898-0.00898 mg·L~(-1))、Cu~(2+)-Zn~(2+)-Cr6+-Mn~(2+)(0.00819-0.00819-0.00819-0.00819 mg·L~(-1))四元联合测试液的作用结果显示为拮抗效应,其余四元联合测试液的作用结果的则均显示是协同效应。Cu~(2+)-Zn~(2+)-Cd~(2+)-Cr~(6+)-Mn~(2+)等毒性(0.00074-1.520-0.348-2.082-8.107 mg·L~(-1))和等浓度(0.00582-0.00582-0.00582-0.00582-0.00582 mg·L-1)配比的五元联合测试液作用结果均显示是协同效应。
关键词(KeyWords)： 重金属;萼花臂尾轮虫;联合毒性;急性毒性
基金项目(Foundation): 国家自然科学基金(31470015);; 安徽省高校生物环境与生态安全省级重点实验室专项基金(2004sys003);; 安徽省高等学校自然科学研究重点项目(KJ2016A063)
作者(Author): 徐晓平;席贻龙;黄林;
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DOI:
参考文献(References)：
- [1]王学锋,朱桂芬.重金属污染研究新进展[J].环境科学与技术,2003,26(1):54-56Wang X F,Zhu G F.Recent progress in studies of heavy metals pollution[J].Environmental Science and Technology,2003,26(1):54-56(in Chinese)
- [2]郑振华,周培疆,吴振斌.复合污染研究的新进展[J].应用生态学报,2001,12(3):469-473Zheng Z H,Zhou P J,Wu Z B.New advances in research of combined pollution[J].Chinese Journal of Applied Ecology,2001,12(3):469-473(in Chinese)
- [3]Norwood W P,Borgmann U,Dixon D G,et al.Effects of metal mixtures on aquatic biota:A review of observations and methods[J].Human and Ecological Risk Assessment,2003,9(4):795-811
- [4]Wallace R L,Snell T W.Phylum Rotifera[M]//Thorp J H,Covich A P.Eds.Ecology and Classification of North American Freshwater Invertebrates.San Diego,CA:Academic Press,2001:195-254
- [5]Xu X P,Xi Y L,Huang L,et al.The life table demographic response of freshwater rotifer Brachionus calyciflorus to multi-metal(Cu,Zn,Cd,Cr,and Mn)mixture interaction[J].Bulletin of Environmental Contamination and Toxicology,2014,93:165-170
- [6]徐吉洋,张文萍,李少南,等.萼花臂尾轮虫毒性试验的方法学研究[J].生态毒理学报,2015,10(2):346-352Xu J X,Zhang W P,Li S N,et al.Research on toxicity test method for Brachionus calyciflorus[J].Asian Journal of Ecotoxicology,2015,10(2):346-352(in Chinese)
- [7]Dahms H U,Hagiwara A,Lee J S.Ecotoxicology,ecophysiology,and mechanistic studies with rotifers[J].Aquatic Toxicology,2011,101:1-12
- [8]American Society for Testing and Material(ASTM).Standard Guide for Acute Toxicity Tests with the Rotifer Brachionus.Annual Book of ASTM Standards[S].Philadelphia,PA,USA:ASTM,1991:E1440-91
- [9]Organization for Economic Co-operation and Development(OECD).Detailed Review Paper on Aquatic Arthropods in Life-cycle Toxicity Tests with An Emphasis on Developmental,Reproductive and Endocrine Disruptive Effects[M].Paris:OECD,2006:125
- [10]张融,范文宏,唐戈,等.水体中重金属镉和锌对大型蚤联合毒性效应的初步研究[J].生态毒理学报,2008,3(3):286-290Zhang R,Fan W H,Tang G,et al.A preliminary study on joint toxic effects of Cd and Zn on Daphnia magna[J].Asian Journal of Ecotoxicology,2008,3(3):286-290(in Chinese)
- [11]宋志慧,王庆伟.Cu2+,Cd2+和Cr6+对斑马鱼联合毒性作用和生物预警的研究[J].生态毒理学报,2011,6(4):361-366Song Z H,Wang Q W.Study on joint toxicity and biological early warning of Cu2+,Cd2+and Cr6+using zebrafish[J].Asian Journal of Ecotoxicology,2011,6(4):361-366(in Chinese)
- [12]刘华,刘可慧,周振明,等.Mn、Zn单一及复合污染对水蓼氮素代谢的影响[J].生态环境学报,2015,24(3):517-521Liu H,Liu K H,Zhou Z M,et al.Effects of Mn/Zn single and combined pollution on the nitrogen metabolism in Polygonum hydropiper L[J].Ecology and Environment Sciences,2015,24(3):517-521(in Chinese)
- [13]Snell T W,Moffat B D,Janssen C R,et al.Acute toxicity tests using rotifers:IV.Effects of cyst age,temperature,and salinity on the sensitivity of Brachionus calyciflorus[J].Ecotoxicology and Environmental Safety,1991,21(3):308-317
- [14]赵含英,杨家新,陆正和,等.Cu2+对萼花臂尾轮虫的毒性影响[J].南京师大学报:自然科学版,2002,25(4):81-85Zhao H Y,Yang J X,Lu Z H,et al.The toxic effect ofCu2+on rotifer Brachionus calyciflorus[J].Journal of Nanjing Normal University:Natural Science Edition,2002,25(4):81-85(in Chinese)
- [15]Sarma S S S,Martínez-Jerónimo F,Ramírez-Pérez T,et al.Effect of cadmium and chromium toxicity on the demography and population growth of Brachionus calyciflorus and Brachionus patulus(Rotifera)[J].Journal of Environmental Science and Health Part A,Toxic/Hazardous Substances and Environmental Engineering,2006,41(4):543-558
- [16]United States Environmental Protection Agency(US EPA).Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms(Fifth Edition)[R].Washington DC:Office of Water,Office of Science and Technology,2002
- [17]章宗涉,黄祥飞.淡水浮游生物研究方法[M].北京:科学出版社,1991:410-411
- [18]Wang X M,Chu Z X,Zha F G,et al.Determination of heavy metals in water and tissue of crucian carp(Carassius auratus Gibelio)collected from subsidence pools in Huainan coal fields(China)[J].Analytical Letters,2015,48:861-877
- [19]修瑞琴,许永香,付迎春,等.水生毒理联合效应相加指数法[J].环境化学,1994,13(3):269-271Xiu R Q,Xu Y X,Fu Y C,et al.Additive index of coeffects for aquatic toxicology[J].Evironmental Chemistry,1994,13(3):269-271(in Chinese)
- [20]Making L L.An Approach for Additive Toxicity of Chemical Mixtures.Aquatic Toxicology and Hazard Evaluation[M].Philadelphia:ASTM STP Publication,1977:99-108
- [21]熊治廷.环境生物学[M].北京:化学工业出版社,2010:85-92
- [22]周启星,程云,张倩茹,等.复合污染生态毒理效应的定量关系分析[J].中国科学C辑,2003,33(6):566-573
- [23]赵清清,席贻龙,李志超,等.不同藻密度下Zn2+浓度对萼花臂尾轮虫实验种群增长参数的影响[J].生态学报,2015,35(12):4026-4033Zhao Q Q,Xi Y L,Li Z C,et al.Effects of Zn2+concentration on population growth parameters of Brachionus calyciflorus under different Scenedesmus obliquus densities[J].Acta Ecologica Sinica,2015,35(12):4026-4033
- [24]Altenburger R,Backhaus T,Boedeker W,et al.Simplifying complexity:Mixture toxicity assessment in the last 20 years[J].Environmental Toxicology and Chemistry,2013,32(8):1685-1687
- [25]Yang R,Li N,Ma M,et al.Combined effects of estrogenic chemicals with the same mode of action using an estrogen receptor binding bioassay[J].Environmental Toxicology and Pharmacology,2014,38(3):829-837
- [26]Yang R,Li N,Rao K,et al.Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro[J].Ecotoxicology and Environmental Safety,2015,111:228-235
- [27]Vranken G,Tire C,Heip C.The toxicity of paired metal mixtures to the nematode Monhystera disjuncta(Bastian,1865)[J].Marine Environmental Research,1988,26(3):161-179
- [28]苏永红,唐柱云,曾科.重金属联合毒性研究进展[J].现代农业科技,2007,10:174-175
- [29]修瑞琴,许永香,高世荣,等.砷与镉、锌离子对斑马鱼的联合毒性实验[J].中国环境科学,1998,18(4):349-352Xiu R Q,Xu Y X,Gao S R,et al.Joint toxicity test of arsenic with cadmium and zinc ions to zebrafish,Brachynanio rerio[J].China Environmental Science,1998,18(4):349-352(in Chinese)
- [30]华涛,周启星.Cd-Zn对草鱼(Ctenopharyngodon idellus)的联合毒性及对肝脏超氧化物歧化酶(SOD)活性的影响[J].环境科学学报,2009,29(3):600-606Hua T,Zhou Q X.Joint toxic effects of Cd and Zn on grass carp(Ctenopharyngodon idellus)and SOD activity in livers[J].Acta Scientiae Circumstantiae,2009,29(3):600-606(in Chinese)
- [31]Tao S,Liang T,Cao J,et al.Synergistic effect of copper and lead uptake by fish[J].Ecotoxicology and Environmental Safety,1999,44(2):190-195
- [32]董晓晓,刘腾腾,孔强,等.Cu2+,Cd2+和Se4+对大型溞的单一及联合毒性效应[J].山东科学,2012,25(2):38-43Dong X X,Liu T T,Kong Q,et al.Individual and combine toxicity of Cu2+,Cd2+,and Se4+to Daphnia magna[J].Shandong Science,2012,25(2):38-43(in Chinese)
- [33]丁婵娟,付荣恕.Cu2+,Pb2+和Zn2+对大型溞的单一及联合毒性效应[J].山东师范大学学报:自然科学版,2013,28(1):137-140Ding C J,Fu R S.Individual toxicity and joint toxicity of copper,lead,and znic ionc to Daphnia magna[J].Journal of Shandong Normal University:Nature Science,2013,28(1):137-140(in Chinese)
- [34]王莹,郝家胜,陈娜,等.铅、镉和锌3种重金属离子对水螅的联合毒性研究[J].生命科学研究,2006,10(1):91-94Wang Y,Hao J S,Chen N,et al.Joint toxicity test of heavy metal ions lead,cadmium and zinc to Hydra sp[J].Life Science Research,2006,10(1):91-94(in Chinese)
- [35]Eaton J G.Chronic toxicity of a copper,cadmium and zinc mixture to the fathead minnow(Pimephales promelas Rafinesque)[J].Water Research,1973,7(11):1723-1736