多壁碳纳米管对土壤微生物的生态毒理效应Ecotoxicological Effects of Multi-Wall Carbon Nanotube on Soil Microorganisms
贺涔霖;高飞;卢晓霞;侯珍;张姝;
摘要(Abstract):
以多壁碳纳米管为研究对象,从生化作用、酶活性、微生物数量和群落结构4个方面系统评估其对土壤微生物的影响。设置两组实验,分别为碳纳米管组和对照组。对于碳纳米管组,按1mg碳纳米管·g-1土的浓度将多壁碳纳米管与土样均匀混合,对照组中不加入多壁碳纳米管。定期(每28d)取样测定两组土壤中的各项生态毒理指标。近5个月的实验结果显示,不同指标对多壁碳纳米管的响应不同。土壤呼吸作用初期受抑制但后期恢复,氨化作用初期被促进但后期被抑制,脱氢酶活性发生增强和抑制两次波动,荧光素二乙酸酯酶活性在整个实验期间一直被抑制,微生物量出现先减少后增加再减少的规律,群落结构在实验初期和后期均有较大变化。总体上,多壁碳纳米管对土壤微生物表现了一定的生态毒性,但除荧光素二乙酸酯酶活性外,各毒理效应在统计意义上并不显著(0.05水平)。
关键词(KeyWords): 多壁碳纳米管;土壤微生物;生态毒理效应
基金项目(Foundation): 国家自然科学基金(41071311,41030529,40871214)
作者(Author): 贺涔霖;高飞;卢晓霞;侯珍;张姝;
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DOI:
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