重金属对发光海藻Pyrocystis lunula的毒性研究——一种新型的快速生物毒性测试方法QwikLiteStudy of Heavy Metals Toxicity for Dinoflagellate Pyrocystis lunula——A New Rapid Toxicity Test QwikLite
冯鸣凤;朱琳;陈中智;
摘要(Abstract)：
利用海洋发光藻--新月梨甲藻Pyrocystis lunula的生物发光特性(QwikLite方法),以发光抑制为测试终点,研究了6种重金属离子Zn2+、Cu2+、Pb2+、Cr6+、Cd2+、Hg2+的毒性,24h EC50分别为4.6601mg·L-1、0.0960mg·L-1、1.4935mg·L-1、0.1626mg·L-1、0.1272mg·L-1、0.0048mg·L-1.将该结果与这6种金属离子对其它发光藻、发光菌、糠虾和泥螺的毒性效应进行了比较.结果表明,利用QwikLite方法和Vibrio覱scheri(Microtox方法)测定的6种重金属离子的毒性顺序相似,但QwikLite法比Microtox法的灵敏度高1～2个数量级;发光藻与糠虾和泥螺的灵敏度相似或者更高,与泥螺存在显著的相关性.发光藻毒性测试方法QwikLite由于灵敏度高、操作简便、快速经济等优点,可以作为一种新型的快速生物测试方法.
关键词(KeyWords)： Pyrocystis lunula;生物发光;重金属;毒性
基金项目(Foundation): 国家重大专项(水专项No.2008ZX08526-003)
作者(Author): 冯鸣凤;朱琳;陈中智;
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