蒋玫;李磊;沈新强;许高鹏;杨杰青;

• 1:中国水产科学研究院东海水产研究所
• 2:上海海洋大学

摘要(Abstract)：

为研究苯并[a]芘和菲对缢蛏的毒性效应,将缢蛏(Sinonovacula constricta)分别暴露于浓度为0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1苯并[a]芘溶液和0.45 mg·L-1、0.15 mg·L-1、0.05 mg·L-1菲的溶液中,采用单细胞凝胶电泳实验(彗星实验)技术检测不同暴露时间缢蛏血淋巴细胞的DNA损伤程度,对照组为清洁海水。结果显示,高浓度(0.45 mg·L-1)苯并[a]芘溶液和(0.45 mg·L-1)菲溶液在短期(7 d)内即可导致缢蛏血细胞显著的DNA损伤,并且随苯并芘[a]和菲浓度的增大和暴露时间的延长,DNA损伤程度增加。21 d恢复实验后,各浓度组DNA损伤又均有不同程度的恢复,但中高浓度组(0.45 mg·L-1和0.15 mg·L-1)与对照组仍显著性差异。两种多环芳烃物质对缢蛏血细胞的DNA损伤作用均存在较显著时间-剂量-效应关系。其中,苯并芘[a]对缢蛏血细胞的DNA损伤作用要高于菲。

关键词(KeyWords)： 苯并芘[a]和菲;缢蛏;血细胞;DNA损伤;单细胞凝胶电泳;彗星实验

Abstract:

Keywords:

基金项目(Foundation): 中国水产科学研究院基本科研业务费(2014A02XK01);; 农业部应对溢油关键技术专项(2012-2014);; 中央级公益性科研院所基本科研业务费专项(2014T06);; 国家现代农业产业技术体系建设项目(CARS-48)

作者(Author): 蒋玫;李磊;沈新强;许高鹏;杨杰青;

Email:

DOI:

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