何雪珠;李洪博;胡亚茹;刘馥雯;林森;陆强;林匡飞;

• 1:华东理工大学资源与环境工程学院国家环境保护化工过程环境风险评价与控制重点实验室

摘要(Abstract)：

新烟碱类(neonicotinoids)是一种类似于烟碱的内吸性杀虫剂,选择性作用于昆虫的中枢神经系统。目前,有关新烟碱类杀虫剂对非靶标生物的生态风险引发了全球的广泛关注。本文采用土壤生物秀丽隐杆线虫(Caenorhabditis elegans)作为受试生物,探究2种新烟碱类杀虫剂吡虫啉(imidacloprid)和呋虫胺(dinotefuran) 24 h暴露(1μg·L~(-1)～10 mg·L~(-1))后,对秀丽线虫运动行为、摄食行为以及乙酰胆碱酶(ACh E)活性和相关基因(ace,mtl)转录水平的影响。结果表明,2种新烟碱类杀虫剂均对秀丽线虫的生理指标具有随着浓度增加而抑制的效应,其中头部摆动是最为敏感的生理指标,10μg·L~(-1)吡虫啉暴露水平和100μg·L~(-1)呋虫胺暴露水平时,线虫头摆频率相较于对照组有显著减少(P <0.01),其次是身体弯曲>摄食>AChE活性(吡虫啉)和摄食>AChE活性>身体弯曲(呋虫胺),相关基因转录水平没有发现与浓度具有相关的效应,但在环境浓度下的暴露有明显的变化。新烟碱类杀虫剂对秀丽线虫的神经毒性作用表明了其对土壤中非靶标生物具有一定的生态风险。

关键词(KeyWords)： 新烟碱类杀虫剂;吡虫啉;呋虫胺;秀丽隐杆线虫;神经毒性;生态风险

Abstract:

Keywords:

基金项目(Foundation): 农用地土壤环境基准和容量研究(17DZ1202304)

作者(Author): 何雪珠;李洪博;胡亚茹;刘馥雯;林森;陆强;林匡飞;

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DOI:

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