何雪珠;李洪博;胡亚茹;刘馥雯;林森;陆强;林匡飞;

• 1:华东理工大学资源与环境工程学院国家环境保护化工过程环境风险评价与控制重点实验室

摘要(Abstract)：

新烟碱类(neonicotinoids)是一种类似于烟碱的内吸性杀虫剂,选择性作用于昆虫的中枢神经系统。目前,有关新烟碱类杀虫剂对非靶标生物的生态风险引发了全球的广泛关注。本文采用土壤生物秀丽隐杆线虫(Caenorhabditis elegans)作为受试生物,探究2种新烟碱类杀虫剂吡虫啉(imidacloprid)和呋虫胺(dinotefuran) 24 h暴露(1μg·L~(-1)～10 mg·L~(-1))后,对秀丽线虫运动行为、摄食行为以及乙酰胆碱酶(ACh E)活性和相关基因(ace,mtl)转录水平的影响。结果表明,2种新烟碱类杀虫剂均对秀丽线虫的生理指标具有随着浓度增加而抑制的效应,其中头部摆动是最为敏感的生理指标,10μg·L~(-1)吡虫啉暴露水平和100μg·L~(-1)呋虫胺暴露水平时,线虫头摆频率相较于对照组有显著减少(P <0.01),其次是身体弯曲>摄食>AChE活性(吡虫啉)和摄食>AChE活性>身体弯曲(呋虫胺),相关基因转录水平没有发现与浓度具有相关的效应,但在环境浓度下的暴露有明显的变化。新烟碱类杀虫剂对秀丽线虫的神经毒性作用表明了其对土壤中非靶标生物具有一定的生态风险。

关键词(KeyWords)： 新烟碱类杀虫剂;吡虫啉;呋虫胺;秀丽隐杆线虫;神经毒性;生态风险

Abstract:

Keywords:

基金项目(Foundation): 农用地土壤环境基准和容量研究(17DZ1202304)

作者(Author): 何雪珠;李洪博;胡亚茹;刘馥雯;林森;陆强;林匡飞;

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参考文献(References)：

• [1]Casida J E,Durkin K A.Neuroactive insecticides:Targets,selectivity,resistance,and secondary effects[J].Annual Review of Entomology,2013,58(1):99-117
• [2]Wakita T,Kinoshita K,Yamada E,et al.The discovery of dinotefuran:A novel neonicotinoid[J].Pest Management Science,2003,59(9):1016-1022
• [3]Jeschke P,Nauen R,Schindler M,et al.Overview of the status and global strategy for neonicotinoids[J].Journal of Agricultural&Food Chemistry,2011,59(7):2897-2908
• [4]Bonmatin J M,Giorio C,Girolami V,et al.Environmental fate and exposure;neonicotinoids and fipronil[J].Environmental Science&Pollution Research International,2015,22(1):35-67
• [5]Kunce W,Josefsson S,rberg J,et al.Combination effects of pyrethroids and neonicotinoids on development and survival of Chironomus riparius[J].Ecotoxicology&Environmental Safety,2015,122(2):426-431
• [6]Roessink I,Merga L B,Zweers H J,et al.The neonicotinoid imidacloprid shows high chronic toxicity to mayfly nymphs[J].Environmental Toxicology&Chemistry,2013,32(5):1096-1100
• [7]Azevedopereira H M,Lemos M F,Soares A M.Effects of imidacloprid exposure on Chironomus riparius Meigen larvae:Linking acetylcholinesterase activity to behavior[J].Ecotoxicology&Environmental Safety,2011,74(5):1210-1215
• [8]Sánchez-Bayo F.The trouble with neonicotinoids[J].Science,2014,346(6211):806-807
• [9]吴迟,姜辉,王长宾,等.四种新烟碱类杀虫剂对蜜蜂的急性毒性及初级风险评估[J].农药学学报,2015,17(4):486-491Wu C,Jiang H,Wang C B,et al.Acute toxicity and first tier risk assessment of four neonicotinoid insecticides to honeybees[J].Chinese Journal of Pesticide Science,2015,17(4):486-491(in Chinese)
• [10]Zhou D,Yang J,Li H,et al.Ecotoxicological evaluation of low-concentration bisphenol A exposure on the soil nematode Caenorhabditis elegans and intrinsic mechanisms of stress response in vivo[J].Environmental Toxicology&Chemistry,2016,35(8):2041-2047
• [11]Jadhav K B,Rajini P S.Neurophysiological alterations in Caenorhabditis elegans exposed to dichlorvos,an organophosphorus insecticide[J].Pesticide Biochemistry&Physiology,2009,94(2):79-85
• [12]Wu Q,Nouara A,Li Y,et al.Comparison of toxicities from three metal oxide nanoparticles at environmental relevant concentrations in nematode Caenorhabditis elegans[J].Chemosphere,2013,90(3):1123-1131
• [13]Chen N,Li J,Li D,et al.Chronic exposure to perfluorooctane sulfonate induces behavior defects and neurotoxicity through oxidative damages,in vivo and in vitro[J].Plos One,2014,9(11):e113453
• [14]张燕芬,王大勇.利用模式动物秀丽线虫建立环境毒物毒性的评估研究体系[J].生态毒理学报,2008,3(4):313-322Zhang Y F,Wang D Y.Establishment of toxicity evaluation system using model organism of Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2008,3(4):313-322(in Chinese)
• [15]Boyd W A,Mcbride S J,Freedman J H.Effects of genetic mutations and chemical exposures on Caenorhabditis elegans feeding:Evaluation of a novel,high-throughput screening assay[J].Plos One,2007,2(12):e1259
• [16]Kenneth J,Livak T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCTmethod[J].Method,2001,25:402-408
• [17]Kudelska M M,Holden-Dye L,O'Connor V,et al.Concentration-dependent effects of acute and chronic neonicotinoid exposure on the behaviour and development of the nematode Caenorhabditis elegans[J].Pest Management Science,2017,73:1345-1351
• [18]Williams P L,Dusenbery D B.A promising indicator of neurobehavioral toxicity using the nematode Caenorhabditis elegans and computer tracking[J].Toxicology&Industrial Health,1990,6(3-4):425-440
• [19]Williams P L.Toxicity testing of neurotoxic pesticides in Caenorhabditis elegans[J].Journal of Toxicology&Environmental Health Part B Critical Reviews,2014,17(5):284-306
• [20]Wang K,Qi S,Mu X,et al.Evaluation of the toxicity,ACh E activity and DNA damage caused by imidacloprid on earthworms,Eisenia fetida[J].Bulletin of Environmental Contamination&Toxicology,2015,95(4):475-480
• [21]Wakita T,Yasui N,Yamada E,et al.Development of a novel insecticide,dinotefuran[J].Journal of Pesticide Science,2005,30(2):122-123
• [22]Culetto E,Combes D,Fedon Y,et al.Structure and promoter activity of the 5`flanking region of ace-1,the gene encoding acetylcholinesterase of class A in Caenorhabditis elegans[J].Journal of Molecular Biology,1999,290(5):951-966
• [23]Reichert K,Menzel R.Expression profiling of five different xenobiotics using a Caenorhabditis elegans whole genome microarray[J].Chemosphere,2005,61(2):229-237