王龙;杨益新;高子平;尹洁;刘洋;张长波;汤继华;刘仲齐;

• 1:农业部环境保护科研监测所
• 2:河南农业大学农学院
• 3:湖南省湘阴县农业局

摘要(Abstract)：

水稻根系对镉(Cd)的富集能力和向地上部的转运效率直接决定着水稻地上部的Cd积累量,是影响稻米中镉含量的关键因素。提高水稻根系对Cd的阻控能力有助于降低南方稻区大米镉含量超标的风险。以水稻核心种质资源中的高Cd积累品种‘齐头白谷’和低Cd积累品种‘27760’的幼苗和离体叶片为材料,对其Cd吸收转运特性和耐镉能力进行了比较研究。结果表明,根系细胞壁和原生质体的Cd吸收动力学特征都符合米氏方程,‘齐头白谷’的Cd最大吸收速率F_(max)显著大于‘27760’,Km值无显著差异。当根际环境中的Cd浓度高于0.89μmol·L~(-1)时,‘齐头白谷’的细胞壁和原生质体的Cd积累速率显著高于‘27760’。地上部细胞壁和原生质体的Cd吸收速率与根系可溶性组分中的Cd浓度呈显著的线性正相关。当根系可溶性组分中的Cd浓度相近时,‘齐头白谷’地上部细胞壁和原生质体的Cd积累速率显著高于‘27760’。在0.89~8.9μmol·L~(-1)的Cd溶液中,‘齐头白谷’离体叶片的失绿速度明显低于‘27760’。‘齐头白谷’具有耐Cd能力强、Cd积累速率快和转运效率高的特点。水稻离体叶片的耐Cd能力和幼苗根系的Cd积累能力以及幼苗的Cd转运能力高度相关,它们都可作为快速鉴定低Cd积累品种的生理指标。

关键词(KeyWords)： 镉(Cd);水稻;积累特性;亚细胞分布;离体叶片;叶绿素;基因型

Abstract:

Keywords:

基金项目(Foundation): 2016年中国农科院科技创新工程项目(2016-cxgc-lzq);; 公益性行业(农业)科研专项(201403015)

作者(Author): 王龙;杨益新;高子平;尹洁;刘洋;张长波;汤继华;刘仲齐;

Email:

DOI:

参考文献(References)：

• [1]黄益宗,郝晓伟,雷鸣,等.重金属污染土壤修复技术及其修复实践[J].农业环境科学学报,2013,32(3):409-417.Huang Y Z,Hao X W,Lei M,et al.The remediation technology and remediation practice of heavy metals-contaminated soil[J].Journal of Agro-Environment Science,2013,32(3):409-417(in Chinese)
• [2]程金金,宋静,陈文超,等.镉污染对红壤和潮土微生物的生态毒理效应[J].生态毒理学报,2013,8(4):577-586Cheng J J,Song J,Chen W C,et al.The ecotoxicity effects of cadmium on microorganism in Udic-Ferrosols and Aquic-Cambosols[J].Asian Journal of Ecotoxicology,2013,8(4):577-586(in Chinese)
• [3]Vromman V,Saegerman C,Pussemier L,et al.Cadmium in the food chain near non-ferrous metal production sites[J].Food Additives and Contaminants-P art A Chemistry,Analysis,Control,Exposure and Risk Assessment,2008,25(3):293–301
• [4]吴婧,董欣敏,郑燕芳,等.镉致癌的分子机制研究进展[J].生态毒理学报,2015,10(6):54-61Wu J,Dong X M,Zheng Y F,et al.Recent research progress in molecular mechanisms of cadmium induced carcinogenesis[J].Asian Journal of Ecotoxicology,2015,10(6):54-61(in Chinese)
• [5]罗琼,王昆,许靖波,等.我国稻田镉污染现状·危害·来源及其生产措施[J].安徽农业科学,2014,42(30):10540-10542Luo Q,Wang K,Xu J B,et al.Cadmium pollution situation,harm,source and production measures in paddy field of China[J].Journal of Anhui Agricultural Sciences,2014,42(30):10540-10542(in Chinese)
• [6]程旺大,姚海根,张国平,等.镉胁迫对水稻生长和营养代谢的影响[J].中国农业科学,2005,38(3):528-537Cheng W D,Yao H G,Zhang G P,et al.Effect of cadmium on growth and nutrition metabolism in rice[J].Scientia Agricultura Sinica,2005,38(3):528-537(in Chinese)
• [7]李婧,周艳文,陈森,等.我国土壤镉污染现状、危害及其治理方法综述[J].安徽农学通报,2015,21(24):104-107Li J,Zhou Y W,Chen S,et al.Actualities,damage and management of soil cadmium pollution in China[J].Anhui Agricultural Science Bulletin,2015,21(24):104-107(in Chinese)
• [8]胡莹,黄益宗,段桂兰,等.镉对不同生态型水稻的毒性及其在水稻体内迁移转运[J].生态毒理学报,2012,1(6):664-670Hu Y,Huang Y Z,Duan G L,et al.Cadmium toxicity and its translocation in two ecotype rice cultivars[J].Asian Journal of Ecotoxicology,2012,1(6):664-670(in Chinese)
• [9]王龙,高子平,李文华,等.水稻幼苗镉吸收动力学特性的遗传多样性分析[J].植物生理学报,2016,52(1):125-133Wang L,Gao Z P,Li W H,et al.Genetic diversity of cadmium absorption kinetic characteristics in rice(Oryza sativa)seedlings[J].P lant P hysiology Journal,2016,52(1):125-133(in Chinese)
• [10]Liu Z Q.Research advance on the mechanism of cadmium transport in rice[J].Meteorological and Environmental Research,2014,5(5):48-52
• [11]唐非,雷鸣,唐贞,等.不同水稻品种对镉的积累及其动态分布[J].农业环境科学学报,2013,32(6):1092-1098Tang F,Lei M,Tang Z,et al.Accumulation characteristic and dynamic distribution of Cd in different genotypes of rice(Oryza sativa L.)[J].Journal of Agro-Environment Science,2013,32(6):1092-1098(in Chinese)
• [12]文志琦,赵艳玲,崔冠男,等.营养器官镉积累特性对稻米镉含量的影响[J].植物生理学报,2015,51(8):1280-1286Wen Z Q,Zhao Y L,Cui G N,et al.Effects of cadmium accumulation characteristics in vegetative organs on cadmium content in gains of rice[J].P lant P hysiology Journal,2015,51(8):1280-1286(in Chinese)
• [13]Kim D Y,Bovet L,Maeshima M,et al.The ABC transporter At P DR8 is a cadmium extrusion pump conferring heavy metal resistance[J].The P lant Journal,2007,50(2):207-218
• [14]段德超,明革,积炎.植物对铅的吸收、转运、累积和解毒机制研究进展[J].应用生态学报,2014,25(1):287-296Duan D C,Ming G,Ji Y.Research advances in uptake,translocation,accumulation and detoxification of P b in plants[J].Chinese Journal of Applied Ecology,2014,25(1):287-296(in Chinese)
• [15]Leita L,De Nobili M,Cesco S,et al.Analysis of intercellular cadmium forms in roots and leaves of bush bean[J].Journal of Plant Nutrition,1996,19(4):527-533
• [16]Haynes R J.Ion exchange properties of roots and ionic interactions within the root apoplasm:Their role in ion accumulation by plants[J].The Botanical Review,1980,46(1):75-99
• [17]刘清泉,陈亚华,沈振国,等.细胞壁在植物重金属耐性中的作用[J].植物生理学报,2014,50(5):605-611Liu Q Q,Chen Y H,Shen Z G,et al.Roles of cell wall in plant heavy metal tolerance[J].P lant P hysiology Journal,2014,50(5):605-611(in Chinese)
• [18]郑绍建.细胞壁在植物抗营养逆境中的作用及其分子生理机制[J].中国科学,2014,44(4):334-341Zheng S J.The role of cell wall in plant resistance to nutritional stresses and the underlying physiological and molecular mechanisms[J].Science China,2014,44(4):334-341(in Chinese)
• [19]Cobbett C S.Phytochelatins and their roles in heavy metal detoxification[J].P lant P hysiology,2000,123(3):825-832
• [20]Clemens S.Evolution and function of phytochelatin synthases[J].Journal of P lant P hysiology,2006,163(3):319-332
• [21]Petraglia A,Benedictis M D,Degola F,et al.The capability to synthesize phytochelatins and the presence of constitutive and functional phytochelatin synthases are ancestral(plesiomorphic)characters for basal land plants[J].Journal of Experimental Botany,2014,65(4):1153-1163
• [22]陆仲烟,刘仲齐,宋正国,等.大麦中镉的亚细胞分布和化学形态及PCs合成的基因型差异[J].农业环境科学学报,2013,32(11):2125-2131Lu Z Y,Liu Z Q,Song Z G,et al.Subcellular distribution and chemical forms of Cd and the synthesis of phytochelatins(P Cs)in different barley genotypes[J].Journal of Agro-Environment Science,2013,32(11):2125-2131(in Chinese)
• [23]Willats W G T,Knox J P,Mikkelsen J D.Pectin:New insights into an old polymer are starting to gel[J].Trends Food Science&Technology,2006,17(3):97-104
• [24]Pelloux J,Rusterucci C,Mellerowicz E J.New insight into pectin methylesterase structure and function[J].Trends in P lant Science,2007,12(6):267-277
• [25]Morel M,Crouzet J,Gravot A,et al.At HMA3,a P1BATP ase allowing Cd/Zn/Co/P b vacuolar storage in Arabidopsis[J].P lant P hysiology,2009,149(2):894-904
• [26]Weigel H J,Jager H J.Subcellular distribution and chemical form of cadmium in bean plants[J].P lant P hysiology,1980,65(3):480-482
• [27]Wang X,Liu Y,Zeng G,et al.Subcellular distribution and chemical forms of cadmium in Bechmeria nivea(L.)Gaud[J].Environmental&Experimental Botany,2008,62(3):389-395
• [28]张雯,林匡飞,周健,等.不同硫浓度下叶面施硒对水稻幼苗镉的亚细胞分布及化学形态的影响[J].农业环境科学学报,2014,33(5):844-852Zhang W,Lin K F,Zhou J,et al.Effects of selenium foliar spray on subcellular distribution and chemical forms of cadmium in rice seedlings in different sulfur concentrations[J].Journal of Agro-Environment Science,2014,33(5):844-852(in Chinese)
• [29]温娜,王景安,刘仲齐.利用AMMI模型分析稻米镉含量的基因型与环境互作效应[J].农业环境科学学报,2015,34(5):817-823Wen N,Wang J A,Liu Z Q.Analysis of genotypic and environmental effects on cadmium content in rice by AMMI model[J].Journal of Agro-Environment Science,2015,34(5):817-823(in Chinese)
• [30]张颖,杨迎霞,郏艳红,等.利用离体叶片鉴定杨树耐盐潜力[J].植物学报,2011,46(3):302-310Zhang Y,Yang Y X,Jia Y H,et al.Using leaf sections to identify different salt tolerance potential of poplar plants[J].Chinese Bulletin of Botany,2011,46(3):302-310(in Chinese)
• [31]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:74-77Gao J F.P lant P hysiology Experiment Instruction[M].Beijing:Higher Education P ress,2006:74-77(in Chinese)
• [32]Linger P,Ostwald A,Haensler J.Cannabis sativa L.growing on heavy metal contaminated soil:Growth,cadmium uptake and photosynthesis[J].Biologia P lantarum,2005,49(4):567-576
• [33]Degryse F,Shahbazi A,Verheyen L,et al.Diffusion limitations in root uptake of cadmium and zinc,but not nickel,and resulting bias in the Michaelis Constant[J].Plant Physiology,2012,160(2):1097-1109
• [34]Epstein E,Hagen C E.A kinetic study of the absorption of alkali cations by barley roots[J].P lant P hysiology,1952,27(3):457-474
• [35]王玉倩,汪晓丽,单玉华,等.环境因素对小麦根系非选择性阳离子通道(NSCCs)转运钾的影响[J].植物营养与肥料学报,2010,16(4):913-917Wang Y Q,Wang X L,Shan Y H,et al.Effects of environmental factors on nonselective cation channels mediated potassium uptake of wheat roots[J].P lant Nutrition and Fertilizer Science,2010,16(4):913-917(in Chinese)
• [36]张参俊,尹洁,张长波,等.非选择性阳离子通道对水稻幼苗镉吸收转运特性的影响[J].农业环境科学学报,2015,34(6):1028-1033Zhang S J,Yin J,Zhang C B,et al.Effects of nonselective cation channels on accumulation and transfer of Cd in rice seedlings[J].Journal of Agro-Environment Science,2015,34(6):1028-1033(in Chinese)
• [37]Sansoulet J,Cabidoche Y M,Cattan P.Absorption and transport of nitrate and potassium in an Andosol underbanana(Guadeloupe,French West Indies)[J].European Journal of Soil Science,2007,58(2):478-489
• [38]吴晓丽,罗玉明,徐迎春,等.狭叶香蒲吸收Cd2+的动力学特征[J].广西植物,2008,28(4):511-514Wu X L,Luo Y M,Xu Y C,et al.The kinetics characteristic of Cd2+absorption of Typha angustif olia[J].Guihaia,2008,28(4):511-514(in Chinese)
• [39]Uraguchi S,Mori S,Kuramata M,et al.Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice[J].Journal of Experimental Botany,2009,60(9):2677-2688
• [40]Roth U,von Roepenack-Lahaye E,Clemens S.Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+[J].Journal of Experimental Botany,2006,57(15):4003-4013
• [41]Papoyan A,Pieros M,Kochian L V.Plant Cd2+and Zn2+status effects on root and shoot heavy metal accumulation in Thlaspi caerulescens[J].New P hytologist,2007,175(1):51-58
• [42]赵学强,介晓磊,李有田,等.不同基因型小麦钾离子吸收动力学分析[J].植物营养与肥料学报,2006,12(3):307-312Zhao X Q,Jie X L,Li Y T,et al.Study on potassium uptake kinetics by different genotype wheat[J].Plant Nutrition and Fertilizer Science,2006,12(3):307-312(in Chinese)
• [43]Tester M,Leigh R A.Partitioning of nutrient transport processes in roots[J].Journal of Experimental Botany,2001,52:445-457
• [44]Sasaki A,Yamaji N,Yokosho K,et al.Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice[J].The P lant Cell,2012,24(5):2155-2167
• [45]Takahashi R,Ishimaru Y,Senoura T,et al.The OsNRAMP 1 iron transporter is involved in Cd accumulation in rice[J].Journal of Experimental Botany,2011,62(14):4843-4850
• [46]Pinto E,Ferreira I M.Cation transporters/channels in plants:Tools for nutrient biofortification[J].Journal of P lant P hysiology,2015,179:64-82
• [47]于辉,杨中艺,杨知建,等.不同类型镉积累水稻细胞镉化学形态及亚细胞和分子分布[J].应用生态学报,2008,19(10):2221-2226Yu H,Yang Z Y,Yang Z J,et al.Chemical forms and subcellular and molecular distribution of Cd in two Cdaccumulation rice genotypes[J].Chinese Journal of Applied Ecology,2008,19(10):2221-2226(in Chinese)
• [48]王芳,丁杉,张春华,等.不同镉耐性水稻非蛋白巯基及镉的亚细胞和分子分布[J].农业环境科学学报,2010,29(4):625-629Wang F,Ding S,Zhang C H,et al.Non-proteinthiols,subcellular and molecular distribution of cadmium in two rice cultivars with difference tolerance[J].Journal of Agro-Environment Science,2010,29(4):625-629(in Chinese)
• [49]Sela M,Tel-Or E,Fritz E,et al.Localization and toxic effects of cadmium,copper,and uranium in azolla[J].P lant P hysiology,1988,88(1):30-36
• [50]Ueno D,Yamaji N,Kono I,et al.Gene limiting cadmium accumulation in rice[J].P roceedings of the National Academy of Sciences of the United States of America,2010,107(38):16500-16505
• [51]吕素莲.转bet A和Ts VP基因提高棉花耐盐、抗旱性的研究[D].济南:山东大学,2008:56-58Lv S L.Study on the enhancement of salt and drought tolerance of cotton plants by transgene Bet A and Ts VP[D].Ji’nan:Shandong University,2008:56-58(in Chinese)