汞胁迫对齿肋赤藓生物结皮细胞超微结构的影响Effect of Mercury on Ultrastructure of Mesophyll Cells of Syntrichia caninervis
刘卫国;霍举颂;黄廷温;玛丽亚·努尔兰;张雨;
摘要(Abstract):
细胞器结构是生物细胞功能的基础,而重金属会对细胞器结构产生毒害效应,进而破坏生物的组织器官完整性及生理代谢功能,汞对生物结皮细胞器超微结构的影响尚未见报道。为探究汞胁迫对齿肋赤藓生物结皮叶肉细胞超微结构的影响,将叶肉细胞暴露于不同浓度的汞溶液中(0、10、20、30、40、50和60 mmol·L~(-1)),连续培养7 d,采用透射电镜技术观察叶肉细胞的变化。与对照组相比,汞浓度在20~60 mmol·L~(-1)时,齿肋赤藓生物结皮的细胞器超微结构随汞浓度增加逐渐发生变化,细胞壁逐渐模糊,出现质壁分离现象,液泡出现一定的空泡化,叶绿体膜破损,类囊体、基粒及基质片层消失,细胞核解体及核仁消失。综上所述,齿肋赤藓生物结皮在低汞浓度时可维持有机体免受毒害,表明齿肋赤藓生物结皮有一定的重金属胁迫耐性,但超过阈值(20 mmol·L~(-1))时,出现汞胁迫伤害,且随汞浓度增加,胁迫毒害效应加剧。本研究结果可为干旱区非维管植物(齿肋赤藓)适应重金属胁迫环境理论及抗性物种培养提供参考。
关键词(KeyWords): 汞胁迫;齿肋赤藓生物结皮;生物土壤结皮;超微结构
基金项目(Foundation): 国家自然科学基金项目(31260112);; 新疆联合基金项目(2017D01C058)
作者(Author): 刘卫国;霍举颂;黄廷温;玛丽亚·努尔兰;张雨;
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DOI:
参考文献(References):
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