靳非;穆景利;王冬婉;高船舟;王菊英;

• 1:国家海洋环境监测中心
• 2:大连医科大学

摘要(Abstract)：

原油毒性主要源于多环芳烃(PAHs),而烷基化多环芳烃是PAHs的主要组分,氧化衍生物是PAHs降解和代谢过程所产生的重要产物。为评估比较烷基PAHs和氧化PAHs与其母体化合物对海洋生物的毒性,本研究以菲、3-甲基菲和菲醌为研究对象,对海洋经济鱼种——红鳍东方鲀(Takifugu rubripes)幼鱼进行了肝损伤评价。结果显示,线粒体、内质网是对上述3种化合物最为敏感的细胞器;3种化合物对肝损伤的表现形式不同:1.菲导致线粒体数量增加形态改变、内质网减少纹理模糊、出现脂滴、细胞空泡化、出血,2.3-甲基菲导致内质网减少、出现脂滴、细胞空泡化、出血,3.菲醌导致线粒体和内质网水肿、出现脂滴及细胞空泡化;随化合物浓度升高,肝损伤程度加重;同一浓度下,不同化合物损伤程度也有差异,总体趋势为菲醌>3-甲基菲>菲。上述结果表明,PAHs衍生物的毒性可能强于其母体化合物;除浓度外,化合物的结构可能对其毒性有重要影响。

关键词(KeyWords)： 多环芳烃;肝毒性;超微结构;河鲀

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性科研专项(201305002);; 国家海洋局北海分局渤海溢油项目(SDFZQ201411124-036);; 辽宁省海洋与渔业科研项目(201416)

作者(Author): 靳非;穆景利;王冬婉;高船舟;王菊英;

Email:

参考文献(References)：

• [1]Mu J L,Wang J Y,Jin F,et al.Comparative embryotoxic-ity of phenanthrene and alkyl-phenanthrene to marine medaka(Oryzias melastigma)[J].Marine Pollution Bulletin,2014,85(2):505-515
• [2]Mu J L,Jin F,Ma X D,et al.Comparative effects of biological and chemical dispersants on the bioavailability and toxicity of crude oil to early life stages of marine medaka(Oryzias melastigma)[J].Environmental Toxicology and Chemistry,2014,33(11):2576-2583
• [3]杨晶晶,孟祥科,王鑫,等.非离子氨对红鳍东方鲀的急性毒性研究[J].现代农业科技,2013(6):253-254Yang J J,Meng X K,Wang X,et al.Study on acute toxicity of non-ionic ammonia on Takifugu rubripes[J].Modern Agricultural Sciences and Technology,2013(6):253-254(in Chinese)
• [4]高露姣,黄艳青,夏连军,等.不同养殖模式下红鳍东方鲀的品质比较[J].水产学报,2011,35(11):1668-1676Gao L J,Huang Y Q,Xia L J,et al.Comparison of flesh quality of farmed fugu,Takifugu rubripes from different culture models[J].Journal of Fisheries of China,2011,35(11):1668-1676(in Chinese)
• [5]梁艳.鱼体内PAHs生物标志物研究综述[J].上海环境科学,2010,29(4):157-160Liang Y.An overview of the study on PAHs biomarkers in fish body[J].Shanghai Environmental Science,2010,29(4):157-160(in Chinese)
• [6]鲁双庆,刘少军,刘红玉,等.Cu2+或表面活性剂AE对黄鳝肝损伤的超微结构观察[J].应用与环境生物学报,2003,9(2):167-170Lu S Q,Liu S J,Liu H Y,et al.Ultrastructural obvervation of Monopterus albus liver cells exposed to Cu2+or surfantant AE pollution[J].Chinese Journal of Applied&Environmental Biology,2003,9(2):167-170(in Chinese)
• [7]Bras M,Queenan B,Susin S A.Programmed cell death via mitochondria:Different modes of dying[J].Biochemistry(Mosc),2005,70(2):231-239
• [8]Sole M,Porte C,Albaiges J.Mixed-function oxygenase system components and antioxidant enzymes in different marine bivalves:Its relation with contaminant body burdens[J].Aquatic Toxicology,1994,30(3):271-283.
• [9]李泽君,呼丹,熊克朝,等.活性氧与线粒体损伤研究概述[J].中南药学,2014,12(10):989-993Li Z J,Hu D,Xiong K C,et al.A brief overview between reactive oxygen species and mitochondrial injury[J].Central South Pharmacy,2014,12(10):989-993(in Chinese)
• [10]Avery S V.Molecular targets of oxidative stress[J].Biochemical Journal,2011,434(2):201-210
• [11]Haeberlein S L.Mitochondrial function in apoptotic neuronal cell death[J].Neurochemical Research,2004,29(2):521-530
• [12]Ma Q,Fang H,Shang W,et al.Superoxide flashes:Early mitochondrial signals for oxidative stress-induced apoptosis[J].Journal of Biological Chemistry,2011,286(31):27573-27581
• [13]冯涛,魏凤琴,欧阳高亮,等.苯并(a)芘对大弹涂鱼肝细胞超微结构的影响[J].应用生态学报,2003,14(10):1780-1782Feng T,Wei F Q,Ouyang G L,et al.Effects of Ba P exposure on ultrastructures of hepatic cells of Boleophthalmus pectinirostris[J].Chinese Journal of Applied Ecology,2003,14(10):1780-1782(in Chinese)
• [14]刘冰,周忠良.镉对弹涂鱼肝细胞超微结构影响的初步观察[J].水产学报,2007,31(增刊):29-33Liu B,Zhou Z L.Effects of cadmium on the ultrastructure of hepatic cells of Periophthalmus modestus[J].Journal of Fisheries of China,2007,31(Suppl):29-33(in Chinese)
• [15]蒋闰兰,肖佰财,禹娜,等.多环芳烃对水生动物毒性效应的研究进展[J].海洋渔业,2014,36(4):372-384Jing R L,Xiao B C,Yu N,et al.Research advance in toxic effects of PAHs on aquatic animals[J].Marine Fisheries,2014,36(4):372-384(in Chinese)
• [16]Jiraungkoorskul W,Upatham E S,Kruatrachue M,et al.Biochemical and histopathological effects of glyphosate herbicide on Nile Tilapia(Oreochromis niloticus)[J].Environmental Toxicology,2003,18(4):260-268
• [17]Liao C Y,Fu J J,Shi J B,et al.Methylmercury accumulation,histopathology effects,and cholinesterase activity alterations in medaka(Oryzias latipes)following sublethal exposure to methylmercury chloride[J].Environmental Toxicology and Pharmacology,2006,22(2):225-233
• [18]吴玲玲,陈玲,张亚雷,等.菲对斑马鱼鳃和肝组织结构的影响[J].生态学杂志,2007,26(5):688-692Wu L L,Chen L,Zhang Y L,et al.Effects of phenanthrene toxicity on histopathology of Brachydanio rerio gill and liver[J].Chinese Journal of Ecology,2007,26(5):688-692(in Chinese)
• [19]van Dyk J C,Pieterse G M,van Vuren J H.Histological changes in the liver of Oreochromis mossambicus(cichlidae)after exposure to cadmium and zinc[J].Ecotoxicology and Environmental Safety,2007,66(3):432-440
• [20]Weber L J.Aquatic Toxicology[M].New York:Raven Press,1982:55-105
• [21]Wester P W,Canton J H.Histopathological study of Oryzias latipex(medaka)after long-termβ-hexachlorocyclohexane exposure[J].Aquatic Toxicology,1986,9(1):21-45