闫会萍;张弛;杜乐;宋小波;丁孝民;魏东洋;许振成;陆一帆;

• 1:北京体育大学运动康复系
• 2:环境保护部华南环境科学研究所

摘要(Abstract)：

为了研究运动对2,3,7,8-四氯二苯并二恶英(2,3,7,8-TCDD)急性暴露大鼠肝组织酶活性的影响,将40只雄性Wistar大鼠随机分为正常对照组(NC)、染毒组(NT)、运动对照组(EC)、运动染毒组(ET)。染毒组(NT组与ET组)腹腔注射10μg·kg-1(以单位体重计)的TCDD,对照组(NC组与EC组)腹腔注射等量的玉米油;NT、NC组静养4周,ET、EC组运动(尾部负重5%游泳30分钟)4周。4周后,称重并宰杀大鼠,分离肝组织,称重后-80℃保存待测7-乙氧基异吩恶唑酮脱乙基酶(EROD)、7-乙氧基香豆素-O-脱乙基酶(ECOD)及芳香烃羟化酶(AHH)的活性。将数据进行多因素方差分析(MAVONA)处理,结果表明,染毒可降低大鼠体重,增加肝湿重和肝相对重量、增加EROD、ECOD活性;运动可增加大鼠肝相对重量、增加AHH的活性;染毒后运动可降低EROD、ECOD的活性。结论:急性10μg·kg-1(以单位体重计)TCDD染毒后4周可增加大鼠肝相对重量;4周的运动能有效降低TCDD对EROD、ECOD活性的激活作用。

关键词(KeyWords)： 2,3,7,8-四氯二苯并二恶英(2,3,7,8-TCDD);大鼠;肝毒性;肝微粒体酶;运动

Abstract:

Keywords:

基金项目(Foundation): 北京体育大学自主科研课题(2014YB015);; 全国重点地区环境与健康专项调查(21111011101EHH(2011)-505)

作者(Author): 闫会萍;张弛;杜乐;宋小波;丁孝民;魏东洋;许振成;陆一帆;

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