许贻斌;郑惠东;陈宇锋;郑盛华;陈小红;钟硕良;

• 1:福建省水产研究所
• 2:福建省海洋生物增养殖与高值化利用重点实验室

摘要(Abstract)：

采用不同质量浓度的溴氰菊酯(0.0070 mg·L~(-1)、0.014 mg·L~(-1)、0.020 mg·L~(-1)、0.027 mg·L~(-1))对菲律宾蛤仔进行20 d半静置染毒,测定不同时间淋巴液中乙酰胆碱酯酶(ACh E)和钠离子-钾离子-三磷酸腺苷酶(Na~+-K~+-ATPase)活性、鳃和肝脏中谷胱甘肽转硫酶(GST)活性的变化,并观察染毒20 d后鳃丝组织和消化盲囊组织的损伤情况。酶活性分析结果显示,与对照组相比,低浓度组(0.0070 mg·L~(-1))试验期间酶活性均无显著差异(P>0.05);中浓度组(0.014 mg·L~(-1)、0.020 mg·L~(-1))淋巴液中ACh E和Na~+-K~+-ATPase均呈先激活后抑制的变化规律(P<0.05),鳃和肝脏中GST活性均呈上升趋势(P<0.05);高浓度组(0.027mg·L~(-1))淋巴液中ACh E和Na~+-K~+-ATPase、肝脏中GST活性在试验期间持续下降(P<0.01),而鳃中GST活性呈先抑制后升高的趋势(P<0.05)。研究表明,低中浓度的溴氰菊酯对菲律宾蛤仔体内的酶活性表现为先诱导后抑制,具有明显的时间、剂量效应;高浓度的溴氰菊酯对菲律宾蛤仔体内酶活持续抑制,且染毒浓度越高,组织细胞变异越显著,表现为鳃丝上皮细胞纤毛层萎缩、纤毛脱落,消化盲囊上皮细胞膨胀,出现包涵体样结构。

关键词(KeyWords)： 溴氰菊酯;菲律宾蛤仔;酶活性;组织损伤

Abstract:

Keywords:

基金项目(Foundation): 国家海洋局海洋公益性行业科研专项(201005012);; 福建省属公益类科研院所基本科研专项(闽海渔科2013R001-3)

作者(Author): 许贻斌;郑惠东;陈宇锋;郑盛华;陈小红;钟硕良;

Email:

DOI:

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