溴氰菊酯对菲律宾蛤仔体内酶活性和组织损伤的初步探索Preliminary Exploration on the Effects of Deltamethrin on Enzyme Activity and Organs Injury in Ruditapes philippinarum
许贻斌;郑惠东;陈宇锋;郑盛华;陈小红;钟硕良;
摘要(Abstract):
采用不同质量浓度的溴氰菊酯(0.0070 mg·L~(-1)、0.014 mg·L~(-1)、0.020 mg·L~(-1)、0.027 mg·L~(-1))对菲律宾蛤仔进行20 d半静置染毒,测定不同时间淋巴液中乙酰胆碱酯酶(ACh E)和钠离子-钾离子-三磷酸腺苷酶(Na~+-K~+-ATPase)活性、鳃和肝脏中谷胱甘肽转硫酶(GST)活性的变化,并观察染毒20 d后鳃丝组织和消化盲囊组织的损伤情况。酶活性分析结果显示,与对照组相比,低浓度组(0.0070 mg·L~(-1))试验期间酶活性均无显著差异(P>0.05);中浓度组(0.014 mg·L~(-1)、0.020 mg·L~(-1))淋巴液中ACh E和Na~+-K~+-ATPase均呈先激活后抑制的变化规律(P<0.05),鳃和肝脏中GST活性均呈上升趋势(P<0.05);高浓度组(0.027mg·L~(-1))淋巴液中ACh E和Na~+-K~+-ATPase、肝脏中GST活性在试验期间持续下降(P<0.01),而鳃中GST活性呈先抑制后升高的趋势(P<0.05)。研究表明,低中浓度的溴氰菊酯对菲律宾蛤仔体内的酶活性表现为先诱导后抑制,具有明显的时间、剂量效应;高浓度的溴氰菊酯对菲律宾蛤仔体内酶活持续抑制,且染毒浓度越高,组织细胞变异越显著,表现为鳃丝上皮细胞纤毛层萎缩、纤毛脱落,消化盲囊上皮细胞膨胀,出现包涵体样结构。
关键词(KeyWords): 溴氰菊酯;菲律宾蛤仔;酶活性;组织损伤
基金项目(Foundation): 国家海洋局海洋公益性行业科研专项(201005012);; 福建省属公益类科研院所基本科研专项(闽海渔科2013R001-3)
作者(Author): 许贻斌;郑惠东;陈宇锋;郑盛华;陈小红;钟硕良;
Email:
DOI:
参考文献(References):
- [1]朱良天.农药[M].北京:化学工业出版社,2004:120-150
- [2]陈家长,吴伟,瞿建宏.溴氰菊酯胁迫下罗非鱼组织中过氧化氢酶和单胺氧化酶的变化[J].农业环境科学学报,2006,25(6):1441-1445Chen J Z,Wu W,Qu J H,et al.Activities of catalase and monoamine oxidase in different tissues of tilapia under stress of deltamethrin[J].Journal of Agro-Environment Science,2006,25(6):1441-1445(in Chinese)
- [3]夏伟.溴氰菊酯对鲫鱼的分子毒理效应研究[D].武汉:华中师范大学,2008:1-44Xia W.Molecular toxicology of deltamethrin to Carassius auratus[D].Wuhan:Central China Normal University,2008:1-44(in Chinese)
- [4]周志刚,王明学,吕敢堂.溴氰菊酯对草鱼鱼种脑ACh E及ATP酶活性的影响[J].华中农业大学学报,1999(2):176-179Zhou Z G,Wang M X,Lu G T.The effects of decamethrin on brain ACh E and ATPase activities of grass carp(Ctenopharygodon idel us)fingerlings[J].Journal of Huazhong Agricultural University,1999(2):176-179(in Chinese)
- [5]魏华,吴楠,沈竑,等.溴氰菊酯对克氏原螯虾的氧化胁迫效应[J].水产学报,2010,34(5):733-739Wei H,Wu N,Shen H,et al.Oxidative stress of deltamethrin to the liver of crayfish(Procambarus clarkii)[J].Journal of Fisheries of China,2010,34(5):733-739(in Chinese)
- [6]张喆,马胜伟,王贺威,等.十溴联苯醚(BDE-209)对菲律宾蛤仔外套膜抗氧化酶活性的影响[J].生态学杂志,2013,32(1):122-128Zhang Z,Ma S W,Wang H W,et al.Effects of decabromodiphenyl ether(BDE-209)on the antioxidant enzyme activities of Lutjanus argentimaculatus mantle membrane[J].Chinese Journal of Ecology,2013,32(1):122-128(in Chinese)
- [7]张林宝,吴惠丰,孙伟,等.菲律宾蛤仔对镉、铜暴露的蓄积作用及其抗氧化酶系统的响应研究[J].南方水产科学,2013,9(5):64-70Zhang L B,Wu H F,Sun W,et al.Bioaccumulation and antioxidant responses in Manila clam(Ruditapes philippinarum)under cadmium and copper exposure[J].South China Fisheries Science,2013,9(5):64-70(in Chinese)
- [8]张海丽,边海燕,杨跃志,等.酚类污染物对菲律宾蛤仔抗氧化和解毒系统相关酶活性的影响[J].中国海洋大学学报,2012,42(3):21-26Zhang H L,Bian H Y,Yang Y Z,et al.Effects of phenolic compounds on enzyme activities of antioxidant and detoxification systems in Ruditapes philippinarum[J].Periodical of Ocean University of China,2012,42(3):21-26(in Chinese)
- [9]姜双城.溴氰菊酯对菲律宾蛤仔急性毒性研究[J].福建水产,2010(1):41-45Jiang S C.The acute toxicity study of deltamethrin to Philippinarum ruditapes[J].Journal of Fujian Fisheries,2010(1):41-45(in Chinese)
- [10]闫海燕,曾令兵,罗宇良,等.氰戊菊酯对鲤SOD活性和MDA含量以及肝肾细胞形态的影响[J].华中农业大学学报,2012,31(4):499-505Yan H Y,Zeng L B,Luo Y L,et al.Effect of fenvalerate on activity of SOD,content of MDA and histomorphology of liver and kidney of carp[J].Journal of Huazhong Agricultural University,2012,31(4):499-505(in Chinese)
- [11]谢文平,朱新平,陈昆慈,等.氯氰菊酯对草鱼组织Na+/K+-ATP酶活性及肝、鳃超显微结构的影响[J].中国水产科学,2009,16:120-125Xie W P,Zhu X P,Chen K C,et al.Toxic effects of cypermethrin on Na+/K+-ATP activity and ultramicrostructure of gil and liver tissue in grass carp[J].Journal of Fishery Sciences of China,2009,16:120-125(in Chinese)
- [12]Kakko L,Toimela T,Tahit H.The synaptosomal membrane bound ATPase as a target for the neurotoxic effects of pyrethroids,permethrin and cypermethrin[J].Chemosphere,2003,51:475-480
- [13]徐恩斌,张忠兵,谢渭芬.乙酰胆碱酯酶的研究进展[J].国外医学,2003,23(1):73-75
- [14]张明,李盾,陈仪本,等.乙酰胆碱酯酶分子生物学研究进展[J].农药,2006,45(1):8-11Zhang M,Li D,Chen Y B,et al.Research progress in acetylcholinesterase molecular biology[J].Chinese Journal of Pesticides,2006,45(1):8-11(in Chinese)
- [15]温茹淑,郑清梅,徐鸿飞,等.Hg2+对草鱼鱼种肾、鳃Na+/K+-ATPase及其组织结构的影响[J].江西农业大学学报,2012,34(5):1014-1020Wen R S,Zheng Q M,Xu H F,et al.Effects of Hg2+on the Na+/K+-ATPase and histology of the kidney and gil in grass carp[J].Acta Agriculturae Universitatis Jiangxiensis,2012,34(5):1014-1020(in Chinese)
- [16]Naomi L C,Joseph R B.Cholinesterase inhibition and impacts on behavior of the Asian clam,Corbicula fluminea,after exposure to an organophosphate insecticide[J].Aquatic Toxicology,2006,76(3-4):258-267
- [17]Mchenery J G,Linley-Adams G E,Moore D C,et al.Experimental and field studies of effects of dichlorvos exposure on acetylcholinesterase activity in the gil s of the mussel,Mytilus edulis L[J].Aquatic Toxicology,1997,38(1-3):125-143
- [18]谭晓珍,吴垠,李韬,等.氯氰菊酯和氰戊菊酯对栉孔扇贝的急性毒性[J].大连水产学院学报,2005,20(3):207-302Tan X Z,Wu Y,Li T,et al.Acute toxicity of cypermethrin and fenvalerate to scallop(Chlamys farreri)[J].Journal of Dalian Fisheries University,2005,20(3):207-302(in Chinese)
- [19]陈荣,刘辉,李东晓,等.水生动物谷胱甘肽硫转移酶研究进展[J].厦门大学学报:自然科学版,2006,45:176-184Chen R,Liu H,Li D X,et al.A review of gluthione Stransferase in aquatic animal[J].Journal of Xiamen University:Natural Science,2006,45:176-184(in Chinese)
- [20]Vander Oost R,Beyer J,Vermeulen N P E,et al.Fish bioaccumulation and biomarkers in environmental risk assessment[J].Environmental Toxicology and Pharmacology,2003,13:57-149
- [21]陈家长,孟顺龙,瞿建宏,等.低浓度阿特拉津对鲫鱼谷胱甘肽-S转移酶(GSTs)活性的影响[J].生态与农村环境学报,2007,23(1):68-72Chen J Z,Meng S L,Qu J H,et al.Effects of atrazine on activity of glutathione S-transferases in Carassius auratus[J].Journal of Ecology and Rural Environment,2007,23(1):68-72(in Chinese)
- [22]任加云,李树峰.多氯联苯(PCB1254)对栉孔扇贝消化盲囊和鳃丝EROD、GST酶活力的影响[J].中国水产科学,2008,15(2):342-345Ren J Y,Li S F.Effects of polychlorinated biphenyls 1254(PCB1254)on two enzyme activity of biotransformation in digestive gland and gil s of scallop Chlamys ferrari[J].Journal of Fishery Sciences of China,2008,15(2):342-345(in Chinese)
- [23]陈颖.四溴双酚A(TBBPA)对翡翠贻贝(Perna viridis)的亚慢性毒性效应[D].厦门:厦门大学,2007:1-70Chen Y.The subchronic toxic effects on mussel(Perna viridis)with its exposure to bromobisphenol[D].Xiamen:Xiamen University,2007:1-70(in Chinese)
- [24]张先勇,刁晓平,王轶,等.苯并[a]芘对马氏珠母贝肝组织抗氧化酶活性的影响[J].生态毒理学报,2011,6(2):171-175Zhang X Y,Diao X P,Wang Y,et al.Effects of B[a]P on activity of antioxidant enzyme in liver of Pinctada martensi[J].Asian Journal of Ecotoxicology,2011,6(2):171-175(in Chinese)
- [25]陈彩芳,沈伟良,霍礼辉,等.重金属离子Cd2+对泥蚶鳃及肝脏细胞显微和超微结构的影响[J].水产学报,2012,36(4):522-525Chen C F,Shen W L,Huo L H,et al.Effects of cadmium on the microstructure and ultrastructure of gil and hepatopancreas in Tegillarca granosa[J].Journal of Fisheries of China,2012,36(4):522-525(in Chinese)
- [26]陈秀荣.氰戊菊酯对草鱼CYP3A活性的影响及组织毒理学研究[D].武汉:华中农业大学,2010:1-59Chen X R.The study of the effect of fenvalerate on the CYP3A activity and histological toxicology on grass carp[D].Wuhan:Huazhong Agricultural University,2010:1-59(in Chinese)
- [27]Velmurugan B,Selvanayagama M,Cengiz E,et al.Histopathology of lambda-cyhalothrin on tissues(gil,kidney,liver and intestine)of Cirrhinus mrigala[J].Environmental Toxicology and Pharmacology,2007,24:286-291