马栋栋;蒋宇霞;杨雷;应光国;史文俊;

• 1:华南师范大学环境研究院广东省化学污染与环境安全重点实验室华南师范大学环境理论化学教育部重点实验室
• 2:中国科学院广州地球化学研究所有机地球化学国家重点实验室

摘要(Abstract)：

雄激素1,4-雄烯二酮(androstadienedione, ADD)和雄烯二酮(androstenedione, AED)主要用于人类和牲畜疾病的预防和治疗。近年来,ADD和AED的大量使用导致其在河流中广泛检出,甚至在多种鱼类体内亦有检出,且浓度较高。ADD和AED已被证实对鱼类具有生殖毒性和发育毒性,但ADD和AED在转录水平上对鱼类的影响鲜有报道。为探究ADD和AED分子水平毒性,本研究考察了斑马鱼胚胎暴露于ADD(4.48、30.0和231 ng·L~(-1))和AED(3.64、21.7和230 ng·L~(-1))144 h后,对其昼夜节律和下丘脑-垂体-性腺轴(hypothalamic-pituitary-gonadal axis, HPG axis)通路中基因转录表达的影响。结果表明,所有浓度的ADD都显著上调了昼夜节律通路中生物钟基因(per1b)、核受体亚族1的D群基因(nr1d2b)、隐花色素基因(cry5)和si:ch211-132b12.7的转录水平,30.0和231 ng·L~(-1)的ADD下调了时钟节律调节因子基因(clocka)和芳香烃受体核转录蛋白样基因(arntl2)的转录水平。3.64 ng·L~(-1)AED显著增强了per1b和nr1d2b的转录表达。此外在HPG轴中,30.0 ng·L~(-1)ADD显著降低了促黄体生成素V亚基基因(lhb)的转录表达水平,而3.64 ng·L~(-1)AED显著上调了lhb的转录表达水平。值得注意的是,4.48ng·L~(-1)ADD和3.64 ng·L~(-1)AED均显著降低了细胞色素P450的11亚族基因(cyp11b)的转录表达水平。上述研究表明,ADD和AED对昼夜节律和HPG轴中相关基因的转录表达有显著性影响,对斑马鱼具有潜在的内分泌干扰风险。

关键词(KeyWords)： 1,4-雄烯二酮;雄烯二酮;斑马鱼胚胎;内分泌干扰;昼夜节律;下丘脑-垂体-性腺轴

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(41807480);; 中国博士后科学基金面上项目(2018M643116);; 华南师范大学研究生创新计划资助项目(2018LKXM018)

作者(Author): 马栋栋;蒋宇霞;杨雷;应光国;史文俊;

Email:

DOI:

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