李辉龙;雷震;吴尔苗;胡艳;吴石金;

• 1:浙江工业大学生物与环境工程学院

摘要(Abstract)：

通过自然土壤法对蚯蚓进行1,2,4-TCB急性暴露实验,研究了不同剂量的1,2,4-TCB对蚯蚓存亡、生长和表皮及肠道粘膜上皮细胞超微结构的影响。结果表明:1,2,4-TCB对蚯蚓14d半致死浓度为890.295mg·L-1;暴露剂量和培养时间均对蚯蚓生长抑制率产生正相关显著影响,但交互作用不显著;暴露于50mg·kg-11,2,4-TCB时,蚯蚓表皮分泌细胞增多,角质层增厚或上角质层损伤,肠道粘膜上皮细胞凹陷形成一定的溃疡面或呈撕裂分散状,第14天时,上角质层和肠道粘膜上皮细胞损伤均有所恢复;当暴露浓度为400mg·kg-1时,蚯蚓的角质层明显增厚,表皮出现大量的分泌细胞,肠上皮组织呈一定程度的撕裂并少量脱落,第14天时角质层变薄,分泌细胞减少,上角质层破损加剧,肠上皮细胞撕裂严重并持续脱落。第14天电镜观察显示:暴露浓度为50mg·kg-1时,肠上皮细胞线粒体嵴不清晰或断裂,结构模糊;暴露浓度为400mg·L-1时,部分线粒体空泡化或内部有絮状沉淀,嵴变短甚至消失。

关键词(KeyWords)： 1,2,4-三氯苯;赤子爱胜蚓;表皮;肠道;超微结构;生态毒理

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(20977087);; 浙江省自然科学基金项目(Y5090054);; 浙江省高校优秀青年教师资助计划2009;; 浙江省公益技术研究社会发展项目(2010C33G2020078)

作者(Author): 李辉龙;雷震;吴尔苗;胡艳;吴石金;

Email:

参考文献(References)：

• [1]刘军,陈旭庚,李家斌,等.1,2,4-三氯苯的研究动态[J].中国工业医学杂志,2002,15(3):161-163Liu J,Chen X G,Li J B,et al.Progress in research on1,2,4-trichlorobenzen[J].Chinese Journal of Industrial Medicine,2002,15(3):161-163(in Chinese)
• [2]Wang M J,Jones K C.Uptake of chlorobenzens by carrots from spiked and sewage sludge-amended soil[J].Environmental Science&Technology,1994,28(7):1260-1267
• [3]张丽丽,胡继伟,杨占南,等.持久性有机污染物(POPs)的研究进展[J].贵州师范大学学报,2007,25(1):107-112Zhang L L,Hu J W,Yang J N,et al.Reviewon progress of persistent organic pollutants[J].Journal of Guizhou Normal University(Natural Sciences),2007,25(1):107-112(in Chinese)
• [4]Carlson G P,Dziezak J D,Johnson K M.Effect of hallogenated benzenes on acetanilide esterase,acetonilide hydroxilase and procaine esterase in rats[J].Research Communications in Chemical Pathology&Pharmacology,1979,25(1):181-184
• [5]范来富,李富君,李昕,等.1,2,4-三氯苯的研究进展[J].中国职业医学,2000,27(5):45-47
• [6]He Y W,Sun TH,Ou Z Q,et al.Fate of1,2,4-trichlorobenzene(1,2,4-TCB)in soil-rice paddy system[J].Chemospher,1996,32(7):1381-1389
• [7]聂志强,李卫建,刘潇威,等.土壤环境中POPs污染现状及治理技术研究进展[J].安徽农业科学,2008,36(15):6478-6480Nie Z Q,Li W J,Liu X W,et al.Current status of POPs contamination in soil environment and the research progress on its treatment technology[J].Journal of Anhui Agricultural Sciences,2008,36(15):6478-6480(in Chinese)
• [8]Lanno R,Wells J,Conder J,et al.The bioavailability of chemicals in soil for earthworms[J].Ecotoxicology and Environmental Safety,2004,57(1):39-47
• [9]OECD.Guidelines for Testing of Chemicals.Test207:Earthworm acute toxicity tests[S].Paris:Organizationfor Economic Co-operation and Development(OECD),1984
• [10]史雅娟,王昕,吕永龙,等.DDT和三氯杀螨醇对蚯蚓的急性和亚急性毒性影响[J].环境科学学报,2006,26(5):851-857Shi Y J,Wang X,Lv Y L,et al.Acute and subtle toxicological effects of DDT and dicofol on earthworms(Eisenia foetida)[J].Acta Scientiae Ciracmstantiae,2006,26(5):851-857(in Chinese)
• [11]中华医学会.临床技术操作规范病理学分册[M].人民军医出版社,2004,7-10,30-31,39-40
• [12]郭永灿,王振中,张友梅,等.重金属对蚯蚓胃肠道上皮细胞超微结构损伤的研究[J].生态学报,1997,17(3):282-287Guo Y C,Wang Z Z,Zhang Y M,et al.Effects of heavy metal pollution in soil on the mucosa epithelia cell ultrastructure injuring of earthworm's gasterointestinal tract[J].Acta Ecologica Sinica,1997,17(3):282-287(in Chinese)
• [13]Smith E N,Carlson G P.Vairous phamacokinetic parameters in relation to enzyme-inducing abilitiesof1,3,4trichlorobenzene and1,2,4-trichlorobenze[J].Journal of Toxicology and Environmental Health,1980,6(4):737-740
• [14]Kato Y,Yamada S,Sato M,et al.Role of2,3,5-trichlorophenylmethylsulfone,a metabolite of1,2,4-trichlorobenzene,in the induction of hepatic microsomal drugmetabolizing enzymes by1,2,4-trichlorobenzene in rats[J].Toxicology and Applied Pharmacology,1993,122(2):214-217
• [15]Nichol A W,Elsbury S,Rousseaux C G.Porphyrin accumulation in sheep bones associated with1,2,4-trichlorobenzene[J].Bulletin of Environmental Contamination and Toxicology,1981,27(1):72-78
• [16]Paoletti M G.The role of earthworms for assessment of sustainability and as bioindicators[J].Agriculture,Ecosystems and Environment,1999,74(1-3):137155
• [17]郑丽莎,史雅娟,孟凡乔,等.硫丹对蚯蚓存活、生长和肠胃线粒体超微结构的影响[J].环境科学学报,2009,29(6):1283-1287Zheng L S,Shi Y J,Meng Q F,et al.Effects of endosulfan on the mortality,growth and enterogastric ultrastructure of earthworms(Eisenia fetida)[J].Acta Scientiae Circumstantiae,2009,29(6):1283-1287
• [18]袁方曜,王玢.有机磷污染农田中蚯蚓的生物指示研究[J].山东农业科学,2004,(2):57-60
• [19]肖亿群,陆雅君,邱江平.白颈环毛蚓皮肤的超微结构[J].上海师范大学学报,2002,31(2):79-85Xiao Y Q,Lu Y J,Qiu J P.An ultrastructural study of musculature in Pheretima californica(Oligoehaeta:Megascoleeidae)[J].Journal of Shanghai Jiaotong University(Agricultural Science),2002,31(2):79-85
• [20]高玉红,孙振钧,孙新胜,等.兽药阿苯哒唑对蚯蚓皮肤和肠道超显微结构的影响[J].环境科学学报,2008,28(12):2579-2582Gao Y H,Sun Z J,Sun X S,et al.Effects of albendazole on the ultrastructure of the skin and intestinalep ithelium of the earthworm[J].Acta Scientiae Ciracmstantiae,2008,28(12):2579-2582
• [21]Yu L,Yu Z,Yuan Z,et al.Toxicological study of two novel pesticides on earthworm Eisenia foetida[J].Chemosphere,1999,39(13):2347-2356
• [22]郭永灿,颜亨梅,赖勤,等.土壤中重金属污染对白颈环毛蚓(Pheretime Califonica)胃肠道粘膜损伤的扫描电镜观察[J].电子显微镜学报,1994,(2):8489
• [23]Gao Y H,Sun Z J,Sun Y,et al.Toxic effects of albendazole on adenosine triphosphatase activity and ultrastructure in Eisenia fetida[J].Ecotoxicology and Environmental Safety,2007,67(3):378-384
• [24]Liu W,Zhou Q X,Li P J,et al.1,2,4-Trichlorobenzene induction of chromosomalaberrations and cell division of roottip cells in Vicia faba seedling[J].Bulletin of Environmental Contamination and Toxicology,2003,71(4):689-697