李辉龙;雷震;吴尔苗;胡艳;吴石金;

• 1:浙江工业大学生物与环境工程学院

摘要(Abstract)：

通过自然土壤法对蚯蚓进行1,2,4-TCB急性暴露实验,研究了不同剂量的1,2,4-TCB对蚯蚓存亡、生长和表皮及肠道粘膜上皮细胞超微结构的影响。结果表明:1,2,4-TCB对蚯蚓14d半致死浓度为890.295mg·L-1;暴露剂量和培养时间均对蚯蚓生长抑制率产生正相关显著影响,但交互作用不显著;暴露于50mg·kg-11,2,4-TCB时,蚯蚓表皮分泌细胞增多,角质层增厚或上角质层损伤,肠道粘膜上皮细胞凹陷形成一定的溃疡面或呈撕裂分散状,第14天时,上角质层和肠道粘膜上皮细胞损伤均有所恢复;当暴露浓度为400mg·kg-1时,蚯蚓的角质层明显增厚,表皮出现大量的分泌细胞,肠上皮组织呈一定程度的撕裂并少量脱落,第14天时角质层变薄,分泌细胞减少,上角质层破损加剧,肠上皮细胞撕裂严重并持续脱落。第14天电镜观察显示:暴露浓度为50mg·kg-1时,肠上皮细胞线粒体嵴不清晰或断裂,结构模糊;暴露浓度为400mg·L-1时,部分线粒体空泡化或内部有絮状沉淀,嵴变短甚至消失。

关键词(KeyWords)： 1,2,4-三氯苯;赤子爱胜蚓;表皮;肠道;超微结构;生态毒理

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(20977087);; 浙江省自然科学基金项目(Y5090054);; 浙江省高校优秀青年教师资助计划2009;; 浙江省公益技术研究社会发展项目(2010C33G2020078)

作者(Author): 李辉龙;雷震;吴尔苗;胡艳;吴石金;

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DOI:

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