重金属胁迫对日本对虾(Penaeus japonicas)胚胎发育的影响The Toxic Effects of Heavy Metals on the Embryonic Development of Penaeus japonicus
姜会超;刘爱英;宋秀凯;孙国华;刘丽娟;
摘要(Abstract):
以受精1 h以内的日本对虾受精卵为实验对象,采用静水试验方法,研究了Zn、Hg、Cu、Pb、As、Cd和Cr 7种重金属对日本对虾胚胎发育的影响。结果表明,在实验浓度范围内,7种重金属中Hg、Cu对日本对虾胚胎发育毒性最大,其次是Zn、As、Cd,Pb和Cr无明显影响。Hg、Cu、Cd、As、Zn对日本对虾胚胎LC50值分别为:0.0177 mg·L-1、0.1070 mg·L-1、1.6057 mg·L-1、3.3682 mg·L-1、8.2644 mg·L-1,致死效应最低可观测效用浓度(LOEC)分别为0.0037 mg·L-1、0.0166 mg·L-1、0.1302 mg·L-1、1.1572 mg·L-1、2.1522 mg·L-1;抑制发育LOEC分别为0.0062 mg·L-1、0.0176 mg·L-1、0.4002 mg·L-1、1.9497 mg·L-1、0.0617 mg·L-1。随着Hg、Cu浓度的升高,胚胎孵化率不断下降,无节幼体死亡率不断上升,在Hg0.0256 mg·L-1浓度组中,胚胎48 h死亡率达到100%。以上结果说明,Hg、Cu等重金属的存在会延缓日本对虾胚胎的发育速度,随着重金属浓度的增加,滞育现象愈加明显,在Cu 0.32 mg·L-1浓度组中,未见无节幼体孵出。
关键词(KeyWords): 重金属;日本对虾;胚胎;可观测效用浓度;半致死浓度
基金项目(Foundation): 山东省科技发展计划项目(2009GG10009044);; 烟台市科技发展计划项目(2011457);; 水生动物营养与饲料“泰山学者”岗位经费资助(2008-2012)
作者(Author): 姜会超;刘爱英;宋秀凯;孙国华;刘丽娟;
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