纳米Zn/Al-水滑石对Hela细胞的氧化应激效应Oxidative Stress Induced by Nanometer-Sized Zn/Al-Hydrotalcite Particles in Hela Cells
辛芳;关蕾;艾汉华;李岩;常青;杨旭;
摘要(Abstract):
为初步探讨纳米Zn/Al-水滑石对Hela细胞的氧化应激效应,采用不同浓度的纳米Zn/Al-水滑石悬液(0、50、100、200、400、800μg·mL-1)对Hela细胞进行染毒,12h后检测Hela细胞中超氧化物歧化酶(SOD)活性和谷胱甘肽(GSH)含量.结果表明,随着纳米Zn/Al-水滑石染毒浓度的升高,Hela细胞SOD活性逐渐降低,较低浓度组(≤100μg·mL-1)与对照组无显著性差异(p>0.05),较高浓度组(≥200μg·mL-1)与对照组存在显著性差异(p<0.01);随着纳米Zn/Al-水滑石染毒浓度的升高,Hela细胞GSH含量也逐渐降低,较低浓度组(≤50μg·mL-1)与对照组无显著性差异(p>0.05),较高浓度组(≥100μg·mL-1)与对照组存在显著性差异(p<0.05或p<0.01);在实验浓度下,Hela细胞SOD活性和GSH含量与纳米Zn/Al-水滑石染毒浓度呈明显的剂量-效应关系.以上结果提示,较高浓度的纳米Zn/Al-水滑石对Hela细胞可产生一定的氧化应激效应,较低浓度的纳米Zn/Al-水滑石则具有一定的生物相容性.
关键词(KeyWords): 纳米Zn/Al-水滑石;Hela细胞;氧化应激效应;超氧化物歧化酶;谷胱甘肽
基金项目(Foundation): 国家自然科学基金项目(No.30570799)
作者(Author): 辛芳;关蕾;艾汉华;李岩;常青;杨旭;
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