辛芳;关蕾;艾汉华;李岩;常青;杨旭;

• 1:华中师范大学生命科学学院环境科学实验室
• 2:华中师范大学物理与材料学院纳米材料研究中心

摘要(Abstract)：

为初步探讨纳米Zn/Al-水滑石对Hela细胞的氧化应激效应,采用不同浓度的纳米Zn/Al-水滑石悬液(0、50、100、200、400、800μg·mL-1)对Hela细胞进行染毒,12h后检测Hela细胞中超氧化物歧化酶(SOD)活性和谷胱甘肽(GSH)含量.结果表明,随着纳米Zn/Al-水滑石染毒浓度的升高,Hela细胞SOD活性逐渐降低,较低浓度组(≤100μg·mL-1)与对照组无显著性差异(p>0.05),较高浓度组(≥200μg·mL-1)与对照组存在显著性差异(p<0.01);随着纳米Zn/Al-水滑石染毒浓度的升高,Hela细胞GSH含量也逐渐降低,较低浓度组(≤50μg·mL-1)与对照组无显著性差异(p>0.05),较高浓度组(≥100μg·mL-1)与对照组存在显著性差异(p<0.05或p<0.01);在实验浓度下,Hela细胞SOD活性和GSH含量与纳米Zn/Al-水滑石染毒浓度呈明显的剂量-效应关系.以上结果提示,较高浓度的纳米Zn/Al-水滑石对Hela细胞可产生一定的氧化应激效应,较低浓度的纳米Zn/Al-水滑石则具有一定的生物相容性.

关键词(KeyWords)： 纳米Zn/Al-水滑石;Hela细胞;氧化应激效应;超氧化物歧化酶;谷胱甘肽

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30570799)

作者(Author): 辛芳;关蕾;艾汉华;李岩;常青;杨旭;

Email:

参考文献(References)：

• Brown D M,Donaldson K,Borm P J,Schins R P,DehnhardtM,Gilmour P,Jimenez L A,Stone V.2004.Calcium andROS-mediated activation of transcription factors and TNF-alphacytokine gene expression in macrophages exposed to ultrafineparticles[J].American Journal of Physiology Lung CellMolecular Physiology,286(2):344-353
• Brumfiel G.2003.Nanotechnology:A little knowledge[J].Nature,424(6946):246-248
• Cui M M,Huang J,Wang J W,Cao Y,Mao C X,Li Y.2008.Effects of multiwalled carbon nanotubes on SOD activitiesand GSH contents in mouse lung and liver tissue[J].AsianJournal of Ecotoxicology,3(2):162-167(in Chinese)
• Derfus A M,Chan W C W,Bhatia S N.2004.Probing thecytotoxicity of semiconductor quantum dots[J].Nano Letters,4(1):11-18
• Dhindsa R S,Plumb-Dhindas P,Thorpe T A.1981.Leafsenescence:correlated with increased levels of membrancepermeability and lipid peroxidation and decreased levels ofsuperoxide dismutase and catalase[J].Journal of ExperimentalBotany,32(1):93-101
• Dong J,Chen Y,Jin Y H,Chen J.2004.Comparison betweenthe effect of pulmonary fibrosis induced by nanosized silicondioxide and normal silicon dioxide[J].Journal of HealthToxicology,18(4):215-217(in Chinese)
• Han X W,Wang Y.2003.Progresses in preparation andapplication of hydrotalcite and hydrotalcite-like materials[J].Jiangsu Chemical Industry,31(2):26-31(in Chinese)
• Kipen H M,Laskin D L.2005.Smaller is not always better:nanotechnology yields nanotoxicology[J].American Journal ofPhysiology Lung Cellular and Molecular Physiology,289(5):696-697
• Lam C W,James J T,McCluskey R,Hunter R L.2004.Pulmonary toxicity of single-wall carbon nanotubes in mice1and90days after intratracheal instillation[J].ToxicologicalSciences,77(1):126-134
• Li N,Sioutas C,Cho A,Schmitz D,Misra C,Sempf J,WangM Y,Oberley T,Froines J,Nel A.2003.Ultrafine particulatepollutants induce oxidative stress and mitochondrial damage[J].Environmental Health Perspectives,111(4):455-460
• Lin W S,Huang Y W,Zhou X D,Ma Y F.2006.In vitrotoxicity of silica nanoparticles in human lung cancer cells[J].Toxicology and Applied Pharmacology,217(3):252-259
• Liu D D,Fu C,Li Y,Xi Z G,Yang X.2008.Study onbiocompatibility of nano-layered double hydroxides with Helacells[J].Asian Journal of Ecotoxicology,3(3):262-267(inChinese)
• Nakayama H,Wada N,Tsuhako M.2004.Intercalation of aminoacids and peptides into Mg-Al layered double hydroxide byreconstruction method[J].International Journal of Pharmaceutics,269(2):469-478
• Nel A,Xia T,Mdler L,Li N.2006.Toxic potential ofmaterials at the nanolevel[J].Science,311(5761):622-627Oberdrster E.2004.Manufactured nanomaterials(fullerenes,C60)induce oxidative stress in the brain of juvenile largemouth bass[J].Environmental Health Perspectives,112(10):1058-1062
• Orbea A,Fahimi H D,Cajaraville M P.2000.Immunolocalizationof four antioxidant enzymes in digestive glands of mollusks andcrustaceans and fish liver[J].Histochemistry and Cell Biology,114(5):393-404
• Peng X H,Huang K L,Jiao F P,Zhao X H,Yu J G.2006.Layered double hydroxides as a drug delivery system—intercalationand controlled release of naproxen[J].Journal of FunctionalMaterials,37(3):415-417(in Chinese)
• Seida Y,Nakano Y.2002.Removal of phosphate by layereddouble hydroxides containing iron[J].Water Research,36(5):1306-1312
• Service R F.2003.Nanomaterials show signs of toxicity[J].Science,300(5617):243
• Soma C E,Dubernet C,Barratt G,Nemati F,Appel M,BenitaS,Couvreur P.1999.Ability of doxorubicin-loaded nanoparticlesto overcome multidrug resistance of tumor cells after their capture bymacrophages[J].Pharmaceutical Research,16(11):1710-1716
• Wang J J,Sanderson B J,Wang H.2007.Cyto-and genotoxicityof ultrafine TiO2particles in cultured human lymphoblastoid cells[J].Mutation Research,628(2):99-106
• Wang L J,Ding W J.2007.Research progress in nanotoxicology[J].Chinese Journal of Pharmacology and Toxicology,21(1):77-80(in Chinese)
• Wang X,Jia G,Wang S,Chang Y X.2005.Research progressin relationship between nanometer materials and health effects[J].Foreign Medical Sciences-Hygine,32(1):1-6(in Chinese)
• Xia M S,Jin M J,Pan J M,Xu Y,Hu C H,Ye Y.2007.Biological effects of layered double hydroxide on Chlorellapyrenoidosa[J].Journal of the Chinese Ceramic Society,35(9):1237-1241(in Chinese)
• Xia S J,Wu Z L.2001.Molecular Toxicology[M].Wuhan:Hubei Science and Technology Press(in Chinese)
• Xiang X L,Li Y,Tian X K,Yang C,Wu Q,Chang Q,Xi ZG,Yang X.2008.Effects of CdSeS nanocrystal on SODactivities and MDA contents in mouse kidney and brain tissue[J].Asian Journal of Ecotoxicology,3(2):168-173(in Chinese)
• Xiao L,Fu C,Liang S S,Mao C X,Xi Z G,Yang X.2007.Cytotoxicity effects of nano-SiO2and normal SiO2particles onHela cells[J].Asian Journal of Ecotoxicology,2(4):435-439(in Chinese)
• Yamawaki H,Iwai N.2006.Mechanisms underlying nano-sizedair-pollution-mediated progression of atherosclerosis:carbon blackcauses cytotoxic injury/inflammation and inhibits cell growth invascular endothelial cells[J].Circulation Journal,70(1):129-140
• Yang G Y,Liu W X.2007.The preparation and application ofhydrotalcite[J].China Non-Metallic Mining Industry Herald,(3):14-17,22(in Chinese)
• Zhang H S,Yang D F,Xi Z G,Yang Z M,Liu H L,Li JW.2006.Study on the oxidative stress of rats induced by benzo[a]pyrene coated onto nano carbon/silicon dioxide particles[J].Asian Journal of Ecotoxicology,1(4):370-374(in Chinese)
• Zhang M,Chen H C,Yang X J,Lu L D,Wang X.2000.Thedevelopment and prospect of nanometer materials[J].Missilesand Space Vehicles,(3):11-16(in Chinese)
• Zhang Q,Kusaka Y,Zhu X,Sato K,Mo Y,Kluz T,Donaldson K.2003.Comparative toxicity of standard nickel andultrafine nickel in lung after intratracheal instillation[J].Journalof Occupational Health,45(1):23-30
• Zhao Y G,Xu J X.2001.Mitochondria,reactive oxygen speciesand apoptosis[J].Progress in Biochemistry and Biophysics,28(2):168-171(in Chinese)
• Zhu G N,Sun K N,Sun X N.2005.Study Process of theSafety of Nanomaterials[J].Orthopaedic Biomechanics Materialsand Clinical Study,2(5):48-50(in Chinese)
• 崔萌萌,黄俊,王江伟,曹毅,毛彩霞,李岩.2008.多壁碳纳米管对小鼠肺脏和肝脏组织SOD活力和GSH含量的影响[J].生态毒理学报,3(2):162-167
• 董静,陈莹,金一和,陈杰.2004.纳米SiO2与常规SiO2粉尘致肺纤维化作用的研究[J].卫生毒理学杂志,18(4):215-217
• 韩小伟,王英.2003.水滑石及类水滑石材料的合成及应用新进展[J].江苏化工,31(2):26-31
• 刘丹丹,付聪,李岩,袭著革,杨旭.2008.纳米水滑石与Hela细胞的生物相容性研究[J].生态毒理学报,3(3):262-267
• 彭霞辉,黄可龙,焦飞鹏,赵学辉,于金刚.2006.水滑石作为药物载体——萘普生的插层和缓释[J].功能材料,37(3):415-417
• 王莉娟,丁文军.2007.纳米毒理研究进展[J].中国药理学与毒理学杂志,21(1):77-80
• 王翔,贾光,王生,常元勋.2005.纳米材料与健康效应关系的研究进展[J].国外医学卫生学分册,32(1):1-6
• 夏枚生,靳明建,潘金敏,徐勇,胡彩虹,叶瑛.2007.水滑石对蛋白核小球藻的生物学效应[J].硅酸盐学报,35(9):1237-1241
• 夏世钧,吴中亮.2001.分子毒理学基础[M].武汉:湖北科学技术出版社
• 项晓玲,李岩,田熙科,杨超,吴曲,常青,袭著革,杨旭.2008.纳米硫硒化镉对小鼠肾脏和脑组织SOD活力和MDA含量的影响[J].生态毒理学报,3(2):168-173
• 肖琳,付聪,梁思斯,毛彩霞,袭著革,杨旭.2007.纳米SiO2与常规SiO2颗粒对Hela细胞的细胞毒性作用[J].生态毒理学报,2(4):435-439
• 杨桂英,刘温霞.2007.水滑石的制备及应用研究[J].中国非金属矿工业导刊,(3):14-17,22
• 张华山,杨丹凤,袭著革,杨在明,刘焕亮,李君文.2006.载带苯并[a]芘的纳米碳/二氧化硅颗粒引起大鼠氧化应激作用的研究[J].生态毒理学报,1(4):370-374
• 张梅,陈焕春,杨绪杰,陆路德,汪信.2000.纳米材料的研究现状及展望[J].导弹与航天运载术,(3):11-16
• 赵云罡,徐建兴.2001.线粒体,活性氧和细胞凋亡[J].生物化学与生物物理进展,28(2):168-171
• 朱广楠,孙康宁,孙晓宁.2005.碳纳米管和几种纳米颗粒材料的安全性研究进展[J].生物骨科材料与临床研究,2(5):48-50