司万童;蒋海明;栗利曼;刘莎;刘菊梅;沈渭寿;朱晓东;

• 1:南京大学环境学院污染控制与资源化利用国家重点实验室
• 2:环境保护部南京环境科学研究所
• 3:内蒙古科技大学生命科学与技术学院

摘要(Abstract)：

本研究以花背蟾蜍(Bufo Raddei)作为实验动物,以某稀土尾矿库周边受污染的水域湿地为研究样地,以相对无污染的小白河黄河湿地自然保护区为对照样地。通过检测两地花背蟾蜍精巢和卵巢的脏器系数、MDA和GSH含量、SOD、GSH-Px、GST、CAT和GR活性、T-AOC等相关指标。研究花背蟾蜍性腺抗氧化酶对该稀土尾矿库污染胁迫的响应效应。结果显示:与黄河湿地相比,稀土尾矿库湿地复合污染对花背蟾蜍精巢和卵巢脏器系数的影响差异不显著。稀土尾矿库花背蟾蜍精巢和卵巢中MDA含量、GPx、GST和CAT活性显著高于黄河湿地(P<0.05),而GSH含量表现出相反的趋势。精巢中T-AOC显著高于卵巢(P<0.01),但相同组织在两地间差异不显著。结果表明该稀土尾矿库复合污染对花背蟾蜍精巢和卵巢抗氧化能力有一定的毒害效应,且精巢和卵巢对污染胁迫的响应有明显的差异性。在应对稀土尾矿库湿地复合污染胁迫时,精巢更倾向于调用SOD-CAT系统的抗氧化机制,而在卵巢内GSH系统发挥作用更大。

关键词(KeyWords)： 尾矿库;花背蟾蜍;精巢;卵巢;脏器系数;抗氧化;毒性效应

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2014FY110800);; 国家自然科学基金(31460142);; 中国博士后科学基金面上项目(2015M571663);; 内蒙古科技大学创新基金青年学术骨干培养专项(2015QNGG05)

作者(Author): 司万童;蒋海明;栗利曼;刘莎;刘菊梅;沈渭寿;朱晓东;

Email:

DOI:

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