司万童;蒋海明;栗利曼;刘莎;刘菊梅;沈渭寿;朱晓东;

• 1:南京大学环境学院污染控制与资源化利用国家重点实验室
• 2:环境保护部南京环境科学研究所
• 3:内蒙古科技大学生命科学与技术学院

摘要(Abstract)：

本研究以花背蟾蜍(Bufo Raddei)作为实验动物,以某稀土尾矿库周边受污染的水域湿地为研究样地,以相对无污染的小白河黄河湿地自然保护区为对照样地。通过检测两地花背蟾蜍精巢和卵巢的脏器系数、MDA和GSH含量、SOD、GSH-Px、GST、CAT和GR活性、T-AOC等相关指标。研究花背蟾蜍性腺抗氧化酶对该稀土尾矿库污染胁迫的响应效应。结果显示:与黄河湿地相比,稀土尾矿库湿地复合污染对花背蟾蜍精巢和卵巢脏器系数的影响差异不显著。稀土尾矿库花背蟾蜍精巢和卵巢中MDA含量、GPx、GST和CAT活性显著高于黄河湿地(P<0.05),而GSH含量表现出相反的趋势。精巢中T-AOC显著高于卵巢(P<0.01),但相同组织在两地间差异不显著。结果表明该稀土尾矿库复合污染对花背蟾蜍精巢和卵巢抗氧化能力有一定的毒害效应,且精巢和卵巢对污染胁迫的响应有明显的差异性。在应对稀土尾矿库湿地复合污染胁迫时,精巢更倾向于调用SOD-CAT系统的抗氧化机制,而在卵巢内GSH系统发挥作用更大。

关键词(KeyWords)： 尾矿库;花背蟾蜍;精巢;卵巢;脏器系数;抗氧化;毒性效应

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2014FY110800);; 国家自然科学基金(31460142);; 中国博士后科学基金面上项目(2015M571663);; 内蒙古科技大学创新基金青年学术骨干培养专项(2015QNGG05)

作者(Author): 司万童;蒋海明;栗利曼;刘莎;刘菊梅;沈渭寿;朱晓东;

Email:

参考文献(References)：

• [1]陈文韬,徐红娇,张乃明,等.某铜矿尾矿库对周围环境影响污染评价研究[J].环境科学导刊,2015,34(2):96-101Chen W T,Xu H J,Zhang N M,et al.Impact assessment of heavy metal pollution of a copper ore mine tailings pond on the surrounding environment[J].Environmental Science Survey,2015,34(2):96-101(in Chinese)
• [2]姚高扬,华恩祥,高柏,等.南方某铀尾矿区周边农田土壤中放射性核素的分布特征[J].生态与农村环境学报,2015(6):963-966Yao G Y,Hua E X,Gao B,et al.Distribution characteristics of radionuclides in soils around tailings dump sites of a uranium mining field in south China[J].Journal of Ecology and Rural Environment,2015(6):963-966(in Chinese)
• [3]马玲,陆宇燕.花背蟾蜍性腺分化的组织学观察[J].沈阳师范大学学报:自然科学版,2011,29(2):288-292Ma L,Lu Y Y.Histological observation of Bufo raddei gonadal differentiation[J].Journal of Shenyang Normal University:Natural Science Edition,2011,29(2):288-292(in Chinese)
• [4]Si W T,Zhang W Y,Lv Y,et al.Heavy metal removal in a constructed wetland and benefits for the development of the toad Bufo raddei[J].Polish Journal of Environmental Studies,2014,23(6):2207-2215
• [5]Huang D J,Zhang Y M,Wang Y J,et al.Assessment of the genotoxicity in toad Bufo raddei exposed to petro chemical contaminants in Lanzhou Region,China[J].Mutation Research,2007,629:81-88
• [6]冯磊,戴昕鹏,张军,等.重金属污染条件下花背蟾蜍跳跃能力与骨骼肌ATP酶和ACh E活性变化研究[J].四川动物,2012,31(4):553-557Feng L,Dai X P,Zhang J,et al.Changes of the locomotor performance and activities of ATPase and ACh E of Bufo raddei under the stress of heavy metal pollution[J].Sichuan Journal of Zoology,2012,31(4):553-557(in Chinese)
• [7]江彬强,陈伟庭,李东风.重金属离子对黑框蟾蜍蝌蚪生长发育的影响[J].华南师范大学学报:自然科学版,2008,5(2):100-105Jiang B Q,Chen W T,Li D F.Effect of heavy metal ion on growth of tadpole of toad(Bufo melanostictus schneide)[J].Journal of South China Normal University:Natural Science Edition,2008,5(2):100-105(in Chinese)
• [8]黄德军,张迎梅,赵东芹,等.重金属镉对花背蟾蜍蝌蚪生长发育的影响[J].兰州大学学报:自然科学版,2004,40(2):81-83Huang D J,Zhang Y M,Zhao D Q,et al.Effect of cadmium on the development of tadpoles of the Bufo raddei[J].Journal of Lanzhou University:Natural Sciences Edition,2004,40(2):81-83(in Chinese)
• [9]周虹,路爱平,黄来旺,等.重金属污染对蛙蟾类毒性的研究进展[J].四川动物,2009,28(5):785-793Zhou H,Lu A P,Huang L W,et al.A review on the toxicity of heavy metal pollution to anura[J].Sichuan Journal of Zoology,2009,28(5):785-793(in Chinese)
• [10]贾秀英,董爱华.镉、铅对蟾蜍精巢毒作用的酶学研究[J].生态学报,2004,24(10):2329-2333Jia X Y,Dong A H.Effects of cadmium and lead on testicular enzymes of Bufo bufo gargarizans[J].Acta Ecologica Sinica,2004,24(10):2329-2333(in Chinese)
• [11]李新红,赵文阁,郭玉民,等.中国林蛙性腺的发育及温度对其性别分化的影响[J].动物学研究,2010,22(5):351-356Li X H,Zhao W G,Guo Y M,et al.Development of sexual gland and influence of temperature on sexual differentiation in Rana chensinensis[J].Zoological Research,2010,22(5):351-356(in Chinese)
• [12]汪美贞,贾秀英.铅对雄性生殖毒性的研究进展[J].动物学杂志,2006,41(1):123-127Wang M Z,Jia X Y.Reproductive toxicity of lead in males[J].Chinese Journal of Zoology,2006,41(1):123-127(in Chinese)
• [13]屠显章,刘学敏,王泽斌,等.包钢尾矿场区潜水弥散试验与水质模型[J].工程勘察,1988(4):28-32Tu X Z,Liu X M,Wang Z B,et al.Groundwater dispersion test and water quality model of the Baotou steel tailing area[J].Engineering Investigation,1988(4):28-32(in Chinese)
• [14]宋刚,张迎梅,朱丽娜,等.黄河上游环境污染对花背蟾蜍抗氧化酶活性及丙二醛含量的影响[J].四川动物,2007,26(2):434-437Song G,Zhang Y M,Zhu L N,et al.Effects of upper yellow river pollution on antioxidant enzymes activities and malondialdehyde content of Bufo raddei[J].Sichuan Journal of Zoology,2007,26(2):434-437(in Chinese)
• [15]Mendoza-Wilson A M,Castro-Arresondo S I,BalandranQuintana R R.Computational study of the structure–free radical scavenging relationship of procyanidins[J].Food Chemistry,2014,161:155-161
• [16]苗菲菲,司万童,刘菊梅,等.尾矿库渗漏水导致泥鳅氧化损伤与DNA损伤的研究[J].广东农业科学,2012,16:162-164Miao F F,Si W T,Liu J M,et al.Study on oxidative damage and DNA damage in Misgurnus anguillicaudatus caused by the tailing lake seepage[J].Guangdong Agricultural Sciences,2012,16:162-164(in Chinese)
• [17]李瑷伶,蓝昕,司万童,等.己烯雌酚对染镉草鱼GSHPx、SOD和MDA影响的研究[J].农业环境科学学报,2008,27(1):350-353Li A L,Lan X,Si W T,et al.Effect of diethylstilbestrol on GSH-Px,SOD and MDA of grass carp exposed to cadmium[J].Journal of Agro-environment Science,2008,27(1):350-353(in Chinese)
• [18]张婧菲,王恬.姜黄素对谷胱甘肽代谢酶诱导及抗氧化机制研究进展[J].食品科学,2012,33(21):359-362Zhang J F,Wang T.Research progress in induction of glutathione metabolic enzymes by curcumin and its antioxidant mechanism[J].Food Science,2012,33(21):359-362(in Chinese)
• [19]Heink A E,Parrish A N,Thorgaard G H,et al.Oxidative stress among SOD-1 genotypes in rainbow trout(Oncorhynchus mykiss)[J].Aquatic Toxicology,2014,156:1-9◆