逯南南;许燕;赵清华;孙莉;石近淼;王明泉;孙韶华;贾瑞宝;

• 1:山东省城市供排水水质监测中心

摘要(Abstract)：

采用离体细胞测试技术,研究三氯乙烯(TCE)、四氯乙烯(PCE)对中国仓鼠卵巢细胞(CHO)的细胞毒性作用。3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)试验结果显示三氯乙烯、四氯乙烯对CHO细胞的半数生长抑制浓度(IC_(50))分别为590 mg·L~(-1)、281 mg·L~(-1)。三氯乙烯、四氯乙烯暴露可导致CHO细胞膜损伤,并且诱导细胞活性氧的产生。经不同浓度的三氯乙烯、四氯乙烯作用24 h后,细胞内超氧化物歧化酶(SOD)活性受到抑制;染毒浓度较低时细胞过氧化氢酶(CAT)活性呈激活势,染毒浓度过高CAT酶活性受到抑制。研究表明在体外培养条件下,氯乙烯类污染物诱导氧化应激可能是其产生细胞毒性的作用机制之一。

关键词(KeyWords)： 氯乙烯;卤代烃;仓鼠卵巢细胞;细胞毒性;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(ZR2014CP019,ZR2014EEQ006,ZR2017MC047);; 国家科技重大专项(2015ZX07406005)

作者(Author): 逯南南;许燕;赵清华;孙莉;石近淼;王明泉;孙韶华;贾瑞宝;

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