郝纪蓉;李云团;江敏;苏怀宇;徐睿;

• 1:上海海洋大学水产与生命学院
• 2:宏德大学农林渔业学院
• 3:上海市水产养殖工程技术研究中心

摘要(Abstract)：

研究了节球藻毒素对斑马鱼(Danio rerio)胚胎的急性毒性效应。采用水浴染毒法,将受精后2h的斑马鱼卵分别暴露于含有0、50、100、200、400、800、1600及3200μg·L-1节球藻毒素的试液中,显微观察不同浓度节球藻毒素对斑马鱼受精卵及其胚胎发育过程的影响,并通过累计孵化率、死亡率、发育畸形率的比较,评价节球藻毒素对斑马鱼胚胎的发育毒性。结果显示:随着节球藻毒素浓度的增加,斑马鱼胚胎的孵化率降低、死亡率和畸形率增加。与对照组比较,浓度≥1600μg·L-1的实验组胚胎发育明显迟缓。浓度≥400μg·L-1时,胚胎孵化率显著降低,72hpf的孵化率由对照组的87%降低到高浓度组(3200μg·L-1)的7%。浓度≥800μg·L-1时,斑马鱼的死亡率、畸形率明显升高;144hpf的死亡率由对照组的7%增加到73%;144hpf的畸形率由对照组的0%增加到93%,畸形主要表现为脊柱弯曲,心包囊肿,尾畸弯曲等。节球藻毒素暴露48hpf对斑马鱼半致死浓度LC50为2572.76μg·L-1,暴露144hpf时为1056.33μg·L-1。节球藻毒素对斑马鱼早期生命发育阶段有一定的抑制效应,高浓度亦会导致死亡。

关键词(KeyWords)： 节球藻毒素;斑马鱼胚胎;毒性效应

Abstract:

Keywords:

基金项目(Foundation): 上海市大学生创新项目(B-5106-11-0012);; 上海市教委重点学科资助项目(J50701);; 上海市高校知识服务平台项目(ZF1206)

作者(Author): 郝纪蓉;李云团;江敏;苏怀宇;徐睿;

Email:

DOI:

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