马强;林爱军;马薇;CHOI Derek;SHIM Hojae;朱永官;

• 1:北京化工大学环境科学与工程系
• 2:北京化工大学环境科学与工程系
• 3:澳门大学
• 4:澳门大学

摘要(Abstract)：

微生物降解和修复是处理土壤中总石油烃(TPH)污染最简单、有效的方法之一.论文阐述了土壤TPH污染的产生、危害以及物理、化学、生物等修复方法的各自特点,其中重点介绍了微生物修复方法,论述了土壤中TPH在微生物表面的吸附、转运,在微生物体内的降解以及相关降解酶及基因;详细介绍了电子受体、温度、pH、营养元素等外界因素对微生物修复TPH污染的影响,在此基础上对土壤TPH污染的微生物修复现状和发展趋势进行了讨论.

关键词(KeyWords)： 土壤污染;总石油烃(TPH);微生物降解;微生物修复

Abstract:

Keywords:

基金项目(Foundation): 澳门大学科技基金项目(No.RG052/06-07S/SHJ/FST);; 澳门政府科技发展基金项目(No.014/2006/A1)

作者(Author): 马强;林爱军;马薇;CHOI Derek;SHIM Hojae;朱永官;

Email:

DOI:

参考文献(References)：

• Al-Daher R,Al-Awadhi N,Ei-Nawawy A.1998.Bioremediationof damaged desert environment using the windrow soil pilesystem in Kuwait[J].Environment International,24:175-180
• Bateman J N,Speer B,Feduik L,Hartline R A.1986.Naphthalene association and uptake in Pseudomonas putida[J].JBacteriol,166(1):155-161
• Cao Y Z,Shi L Y,Li L H,Li F S.2007.Petroleumhydrocarbons-contaminated sites and related risk-based managementstrategy[J].Asian Journal of Ecotoxicology,2(3):265-272(in Chinese)
• Chakrabarty A M,Chou G,Gunsalus I C.1973.Geneticregulation of octane dissimilation plasmid in Pseudomonas[J].Proceedings of the National Academy of Sciences,70(4):1137-1140
• Cheng S Q,Liang F L,Gu X B,Liu R L.2004.Advances inthe genetics of alkane degradation genes(alk)[J].ChinaBiotechnology,24(3):30-34(in Chinese)
• Christian H,Jorg H,Armin H.1999.Detection of polycyclicaromatic hydrocarbon degradation genes in different soil bacteriaby polymerase chain reaction and DNA hybridization[J].FEMSMicrobiology Letters,173:255-263
• Franco M A,Vinas L,Soriano J A,Armas D,Gonzalez J J.2006.Spatial distribution and ecotoxicity of petroleum hydrocarbonsin sediments from the Galicia continental shelf(NW Spain)afterthe Prestige oil spill[J].Marine Pollution Bulletin,53:260-271
• Fredrickson J K,Bolton H,Brockman F J.1993.In-situ andon-site bioreclamation[J].Environmental Science&Technology,27:1711-1716
• Gong Z Q,Li P J,Tai P D,Lin X,Chen S H,Geng C N.2002.Advancement of soil washing process for contaminated soil [J].Techniques and Equipment for Environmental PollutionControl,3(7):45-50(in Chinese)
• Gordon A,Hambrick,Ronald D,Delaune W H,Patrick J R.1980.Effect of estuarine sediment pH and oxidation-reductionpotential on microbial hydrocarbon degradation[J].Applied andEnvironmental Microbiology,8:365-369
• He L J,Li P J,Wei D Z,Wang D Z.2004.Nutrient balanceand mechanism of biological degradation of oil[J].EnvironmentalScicence,25(1):91-94(in Chinese)
• Iwabuchi T,Harayama S.1997.Biochemical and geneticcharacterization of2-carboxybenzaldehyde dehydrogenase,an enzymeinvolved in phenanthrene degradation by Nocardioides sp.strainKP7[J].Journal of Bacteriology,179:6488-6494
• Jiang C L.2001.The process of phy-chemical treatment andbioremediation for petroleum-contaminated soil[D].Institute ofApplied Ecology,Chinese Academy of Sciences(in Chinese)
• Jonathan D,Hamme V,Ward O P.2001.Physical andmetabolic interactions of pseudomonas sp.strain JA5-B45andrhodococcus sp.strain F9-D79during growth on crude oil andeffect of a chemical surfactant on them[J].Applied andEnvironmental Microbiology,10:4874-4879
• Kniemeyer O,Heider J.2001.Ethylbenzene dehydrogenase,anovel hydrocarbon-oxidizing molybdenum/iron-sulfur/heme enzyme[J].J Biol Chem,276(24):21381-21386
• Koren O,Knezevic V,Eliora Z R,Eugene R.2003.Petroleumpollution bioremediation using water-insoluble uric acid as thenitrogen source[J].Applied and Environmental Microbiology,10:6337-6339
• Lee C H,Lee J Y.2002.Evaluation of air injection andextraction test at a petroleum contaminated site[J].Water,Airand Soil Pollution,135:65-97
• Li X W,Liu Z P.2002.Microbial biodegradation of petroleumhydrocarbons[J].Acta Microbiologica Sinica,42(6):764-767(in Chinese)
• Lu X J,Guo S H,Sun Q,Liang C H.2003.Current situationof remediating techniques of petroleum contaminated soil and itsprospects[J].Journal of Shenyang Agricultural University,34(1):63-67(in Chinese)
• Luis G T,Neftali R,Guadalupe B,Rosario I.2005.Effect oftemperature,and surfactants’s HLB and dose over the TPH-diesel biodegradation process in aged soil[J].Process Biochemistry,40:3296-3302
• Luis G T,Neftali R,Rosario I.2004.Use of two-surfactantsmixtures to attain specific HLB values for assisted TPH-dieselbiodegradation[J].Journal of Environmental Sciences,16:950-956
• Margesin R,Schinner F.1999.Biodegradation of diesel oil bycold-adapted microorganisms in presence of sodium dodecylsulfate[J].Chemosphere,38(15):3463-3472
• Nan Z D,Li G Z,Gu Q,Zhan H L,Zen X C.2002.Studieson phase diagram of nonionic surfactant Tween80[J].ChemicalResearch and Application,14(4):401-404(in Chinese)
• Owen W,Ajay S J,Hamme V.2003.Acceleratedbiodegradation of petroleum hydrocarbon waste[J].MicrobiolBiotechnol,30:260-270
• Rabus R,Kube M,Heider J,Beck A,Heitmann K,Widdel F,Reinhardt R.2005.The genome sequence of an anaerobicaromatic-degrading denitrifying bacterium strain EbN1[J].Archivesof Microbiology,183:27-36
• Ruberto L,Vazquez S C,Mac Cormack W P.2003.Effectiveness of the natural bacterial flora,biostimulation andbioaugmentation on the bioremediation of a hydrocarboncontaminated Antarctic soil[J].International Biodeterioration&Biodegradation,52:115-125
• Sanjeet M,Jeevan J,Ramesh C K,Banwari L.2001.Evaluation ofinoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil[J].Applied And EnvironmentalMicrobiology,67(4):1675-1681
• Sanjeet M,Priyangshu M S,Banwari L.2004.Crude oildegradation efficiency of a recombinant Acinetobacter baumanniistrain and its survival in crude oil-contaminated soil microcosm[J].FEMS Microbiology Letters,235:323-331
• Shao F Q,Han Q X.2003.The application of enzymeengineering in the environmental pollution control[J].Journal ofPetrochemical Universities,16(2):36-40(in Chinese)
• Song Y F,Zhou Q X,Song X Y,Zheng W.2004.Pollutantsresidue in soil and the eco-toxicity after irrigation withpetroleum wastewater[J].Chinese Journal of Ecology,23(5):61-66(in Chinese)
• Sun T H,Zhou Q X,Li P J.2001.Pollution Ecology[M].Beijing:Science Press(in Chinese)
• Vinas M,Sabate J,Espuny M J,Solanas A M.2005.Bacterialcommunity dynamics and polycyclic aromatic hydrocarbondegradation during bioremediation of heavily creosote-contaminatedsoil[J].Applied and Environmental Microbiology,11:7008-7018
• Wang H Q,Chen Y J,Sun N N.2006.Mechanism ofpetroleum hydrocarbon biodegradation in contaminated soil andbiorestoration technology[J].Earth Science Frontiers,13(1):134-139(in Chinese)
• Whitman B E,Luekong D R.1998.Naphthalene uptake by aPseudomonas fluorescens isolate[J].Canadian Journal ofMicrobiology,44:1086-1093
• Xie D P,Yin H,Peng H,Ye J S,Zhang N.2004.Degradation of crude oil by miced culture[J].Chinese Journalof Applied and Environmental Biology,10(2):210-214(inChinese)
• Zhang L F,Jiang C Z,Li D H.2001.Study on effect ofsurfactants on biodegradation of petrolum with petroleum-degrading bacterial strains[J].Journal of Shenyang Institute of Technology,20(4):79-83(in Chinese)
• Zhang Y M,Miller R M.1994.Effect of a Pseudomonasrhamnolipid biosurfactant an cell hydrophobicity and biodegradationof Octadecane[J].Applied and Environmental Microbiology,60:2101-2106
• Zhou Q X,Wang M E.2006.Researching advancement andprospect of soil ecotoxicology[J].Asian Journal of Ecotoxicology,1(1):1-11(in Chinese)
• 曹云者,施烈焰,李丽和,李发生.2007.石油烃污染场地环境风险评价与风险管理[J].生态毒理学报,2(3):265-272
• 程守强,梁凤来,顾晓波,刘如林.2004.烷烃降解基因alk研究进展[J].中国生物工程杂志,24(3):30-34
• 巩宗强,李培军,台培东,蔺昕,陈素华,耿春女.2002.污染土壤淋洗法修复研究进展[J].环境污染治理技术与设备,3(7):45-50
• 何良菊,李培杰,魏德州,王淀佐.2004.石油烃微生物降解的营养平衡及降解机理[J].环境科学,25(1):91-94
• 姜昌亮.2001.石油污染土壤的物理化学处理-生物修复工艺与技术研究[D].中国沈阳科学院应用生态研究所.1-20
• 李习武,刘志培.2002.石油烃的微生物降解[J].微生物学报,42(6):764-767
• 陆秀君,郭书海,孙清,梁成华.2003.石油污染土壤的修复技术研究现状及展望[J].沈阳农业大学学报,34(1):63-67
• 南照东,李干佐,顾强,张洪林,曾宪诚.2002.非离子表面活性剂TWEEN80相图的研究[J].化学应用与研究,16(4):401-404
• 邵凤琴,韩庆祥.2003.酶工程在污染治理中的应用[J].石油化工高等学校学报,16(2):36-40
• 宋玉芳,周启星,宋雪英,张薇.2004.石油污灌土壤污染物的残留与生态毒理[J].生态学杂志,23(5):61-66
• 孙铁珩,周启星,李培军.2001.污染生态学[M].北京:科学出版社
• 王红旗,陈延君,孙宁宁.2006.土壤石油污染物微生物降解机理与修复技术研究[J].地学前缘,13(1):134-139
• 谢丹平,尹华,彭辉,叶锦韶,张娜.2004.混合菌对石油的降解[J].应用与环境生物学报,10(2):210-214
• 张丽芳,姜承志,李东辉.2001.表面活性剂对不同石油降解菌除油影响的研究[J].沈阳工业学院学报,20(4):79-83
• 周启星,王美娥.2006.土壤生态毒理学研究进展与展望[J].生态毒理学报,1(1):1-11