张光甫;刘薇;邢立国;胥维昌;金一和;

• 1:大连理工大学环境学院工业生态与环境工程教育部重点实验室
• 2:中化集团沈阳化工研究院有限公司

摘要(Abstract)：

建立人皮肤模型检测杀菌类农药的腐蚀/刺激性,探讨体外皮肤模型替代农药的动物皮肤安全性评价实验的可行性和影响因素。使用人皮肤成纤维细胞与鼠尾胶原混合培养模拟真皮层,在真皮层上接种人角化细胞,经气液培养形成表皮层。通过细胞活性和细胞因子测定鉴别化合物的腐蚀/刺激性,并比较体外皮肤模型和动物测试结果的一致性。结果表明,皮肤模型对十种农药的腐蚀性测试中,与动物安全评价测试结果完全一致。在刺激性试验中,细胞因子白介素-1a(IL-1a)作为检测终点提高了体外皮肤模型的灵敏性和准确性,人皮肤模型和动物测试结果的一致性达到80%。研究表明,构建的人皮肤模型在对杀菌类农药腐蚀/刺激性测试中初步表现出较好的性能,作为动物替代方法进行农药品安全性评价具有良好的应用前景。

关键词(KeyWords)： 人皮肤模型;腐蚀性;刺激性;杀菌类农药;IL-1a

Abstract:

Keywords:

基金项目(Foundation): 中国博士后科学基金(2012M520646);; 国家重大新药创制专项(2013ZX09302304)

作者(Author): 张光甫;刘薇;邢立国;胥维昌;金一和;

Email:

参考文献(References)：

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