四溴双酚A对赤子爱胜蚓的生长和基因表达的影响Effects of Tetrabromobisphenol A on Growth and Gene Expression of Earthworms( Eisenia fetida)
徐湘博;史雅娟;吕永龙;郑晓奇;
摘要(Abstract):
试验采用人工土壤法,通过14 d急性毒性试验,研究了不同剂量TBBPA对赤子爱胜蚓(Eisenia fetida)生长、基因表达的影响。在14 d急性毒性试验中,随着暴露时间和浓度的增加,蚯蚓生长受到了显著抑制,未出现死亡现象。超氧化物歧化酶(SOD)的基因表达量在低浓度(50 mg·kg-1)时受到诱导上调;谷胱甘肽转移酶(GST)的基因表达量在400 mg·kg-1染毒组受到明显诱导,是对照组的8.73倍;热休克蛋白(Hsp70)的基因表达量在50、100和200 mg·kg-1染毒组被诱导上调。基因表达量的变化显示,在50、100、200 mg·kg-1浓度条件下,蚯蚓可以利用自身的抗氧化能力维持体内的动态平衡稳定,使得机体免受损伤。但是在超过400 mg·kg-1浓度后,TBBPA的毒性效应超过了机体的应对能力,使机体遭受损伤,外在特征主要表现为蚯蚓身体蜷缩、变细变硬。从生物标志物角度,基因表达量的变化对TBBPA毒性效应的指示作用较生长抑制率更为敏感。
关键词(KeyWords): 四溴双酚A;赤子爱胜蚓;急性毒性;生长抑制率;基因表达
基金项目(Foundation): 国家自然科学基金资助项目(No.41271487);; 国家国际科技合作专项(No.2012DFA91150)
作者(Author): 徐湘博;史雅娟;吕永龙;郑晓奇;
Email:
DOI:
参考文献(References):
- [1]de Wit C A.An overview of brominated flame retardants in the environment[J].Chemosphere,2002,46(5):583-624
- [2]de Wit C A,Herzke D,Vorkamp K.Brominated flame retardants in the Arctic environment-trends and new candidates[J].Science of the Total Environment,2010,408:2885-2918
- [3]OSPAR.OSPAR List of Chemicals for Priority Action(Update 2007)[S].London:Reference number 2004-12,2007
- [4]Colnot T,Kacew S,Dekant W.Mammalian toxicology and human exposures to the flame retardant 2,2',6,6'-tetrabromo-4,4'-isopropylidenediphenol(TBBPA):Implications for risk assessment[J].Archives of Toxicology,2013,1-21
- [5]Watanabe I,Kashimoto T,Tatsukawa R.Identification of the flame retardant tetrabromobisphenol-A in the river sediment and the mussel collected in Osaka[J].Bulletin of Environmental Contamination and Toxicology,1983,31(1):48-52
- [6]Morris S,Allchin C R,Zegers B N,et al.Distribution and fate of HBCD and TBBPA brominated flame retardants in North Sea estuaries and aquatic food webs[J].Environmental Science&Technology,2004,38(21):5497-5504
- [7]Fujii Y,Nishimura E,Kato Y,et al.Dietary exposure to phenolic and methoxylatedorganohalogen contaminants in relation to their concentrations in breast milk and serum in Japan[J].Environment International,2014,63:19-25
- [8]HloukováV,LankováD,KalachováK,et al.Brominated flame retardants and perfluoroalkyl substances in sediments from the Czech aquatic ecosystem[J].Science of the Total Environment,2014,470:407-416
- [9]Ni H G,Zeng H.HBCD and TBBPA in particulate phase of indoor air in Shenzhen,China[J].Science of the Total Environment,2013,458:15-19
- [10]Cariou R,Antignac J P,Marchand P,et al.New multiresidue analytical method dedicated to trace level measurement of brominated flame retardants in human biological matrices[J].Journal of Chromatography A,2005,100(2):144-152
- [11]Johnson-Restrepo B,Adams D H,Kannan K.Tetrabromobisphenol A(TBBPA)and hexabromocyclododecanes(HBCDs)in tissues of humans,dolphins,and sharks from the United States[J].Chemosphere,2008,70(11):1935-1944
- [12]Ronisz D,Farmen Finne E,Karlsson H,et al.Effects of the brominated flame retardants hexabromocyclododecane(HBCDD),and tetrabromobisphenol A(TBBPA),on hepatic enzymes and other biomarkers in juvenile rainbow trout and feral eelpout[J].Aquatic Toxicology,2004,69(3):229-245
- [13]Kuiper R V,Van den Brandhof E J,Leonards P E G,et al.Toxicity of tetrabromobisphenol A(TBBPA)in zebrafish(Danio rerio)in a partial life-cycle tes[J].Archives of Toxicology,2007,81(1):1-9
- [14]Nakajima A,Saigusa D,Tetsu N,et al.Neurobehavioral effects of tetrabromobisphenol A,a brominated flame retardant,in mice[J].Toxicology Letters,2009,189(1):78-83
- [15]Liang X F,Li W,Martyniuk C J,et al.Effects of dechlorane plus on the hepatic proteome of juvenile Chinese sturgeon(Acipenser sinensis)[J].Aquatic Toxicology.2014
- [16]Linhartova P,Gazo I,Shaliutina-Kolesova A,et al.Effects of tetrabrombisphenol A on DNA integrity,oxidative stress,and sterlet(Acipenser ruthenus)spermatozoa quality variables[J].Environmental Toxicology,2014
- [17]WHO.Environmental Health Criteria 172:Tetrabromobisphenol A and Derivatives[S].Geneva,Switzerland:1995:72
- [18]苏燕,范涤敏,罗义,等.四溴双酚A对鲫鱼血清抗氧化系统的影响[J].南京大学学报,2007,43(2):164-170Su Y,Fan D M,Luo Y,et al.Antioxidant response in serum of carassiusauratus underte trabromobisphenol A exposure[J].Journal of Nanjing University,2007,43(2):164-170(in Chinese)
- [19]Yang S W,Xu F F,Zheng B H,et al.Multibiomarker responses upon exposure to tetrabromobisphenol A in the freshwater fish Carassiusauratus[J].Aquatic Toxicology,2013,142 143:248-256
- [20]Celada L J,Whalen M M.Whalen.The effect of TBBPA on MAP3Ks in human natural killer cells[J].The FASEB Journal,2013,27:1042-1045
- [21]Pullen S,Boecker R,Tiegs G.The flame retardants tetrabromobisphenol A and tetrabromobisphenol A bisallylether suppress the induction of interleukin-2 receptorαchain(CD25)in murine splenocytes[J].Toxicology,2003,184(1):11-22
- [22]Kitamura S,Jinno N,Ohta S,et al.Thyroid hormonalactivity of the flame retardants tetrabromobisphenol A and tetrachlorobisphenol A[J].Biochemical and Biophysical Research Communications,2002,293(1):554-559
- [23]Xue Y G,Gu X Y,Wang X R,et al.The hydroxyl radical generation and oxidative stress for the earthworm Eisenia fetida exposed to tetrabromobisphenol A[J].Ecotoxicology,2009,18(6):693-699
- [24]薛银刚,王晓蓉,顾雪元,等.四溴双酚A对赤子爱胜蚓的急性毒性及抗氧化防御系统酶的影响[J].生态毒理学报,2009,4(01):93-100Xue Y G,Wang X R,Gu X Y,et al.Acute toxicity of tetrabromobisphenol A to earthworms Eisenia fetida and its effects on antioxidant defense system enzymes[J].Asian Journal of Ecotoxicology,2009,4(01):93-100(in Chinese)
- [25]OECD.Guideline for Testing of Chemicals Test 207:Earthworm,Acute Toxicity Tests[S].Paris:Organization for Economic Cooperation and Development(OECD),1984
- [26]Shi Y J,Shi Y J,Wang X,et al.Comparative effects of lindane and deltamethrin on mortality,growth,and cellulase activity in earthworms(Eisenia fetida)[J].Pesticide Biochemistry and Physiology,2007,89(1):31-38
- [27]Brulle F,Mitta G,Cocquerelle C,et al.Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure[J].Environmental Science&Technology,2006,40(8):2844-2850
- [28]Chen C,Xue S G,Zhou Q X,et al.Multilevel ecotoxicity assessment of polycyclic musk in the earthworm Eisenia fetida using traditional and molecular endpoints[J].Ecotoxicology,2011,20(8):1949-1958
- [29]Pfaffl M W.A new mathematical model for relative quantification in real-time RT PCR[J].Nucleic Acids Research,2001,29(9):2002-2007
- [30]Chiu K H,Lin C R,Huang H W,et al.Toxic effects of two brominated flame retardants BDE-47 and BDE-183on the survival and protein expression of the tubificid Monopylephorus limosus[J].Ecotoxicology and Environmental Safety,2012,84:46-53
- [31]Wu N,Herrmann T,Paepke O,et al.Human exposure to PBDEs:Associations of PBDE body burdens with food consumption and house dust concentrations[J].Environmental Science&Technology,2007,41(5):1584-1589
- [32]Ribeiro S,Sousa J P,Nogueira A J A,et al.Effect of endosulfan and parathion on energy reserves and physiological parameters of the terrestrial isopod porcelliodilatatus[J].Ecotoxicology and Environmental Safety,2001,49(2):131-138
- [33]BibicˇA,Drobne D,trus J,Byrne A R.Assimilation of zinc by Porcellioscaber(Isopoda,Crustacea)exposed to zinc[J].Bulletin of Environmental Contamination and Toxicology,2007,58(5):814-821
- [34]Mosleh Y Y,Paris-Palacios S,Couderchet M,et al.Effects of the herbicide isoproturon on survival,growth rate,and protein content of mature earthworms(Lumbricusterrestris L.)and its fate in the soil[J].Applied Soil Ecology,2003,23(1):699-77
- [35]Gonzalez P,Dominique Y,Massabuau J C,et al.Comparative effects of dietary methylmercury on gene expression in liver,skeletal muscle,and brain of the zebrafish(Danio rerio)[J].Environmental Science&Technology,2005,39(11):3972-3980
- [36]Fridovich I.Superoxide dismutases.An adaptation to a paramagnetic gas[J].Journal of Biological Chemical,1989,264(14):7761-7764
- [37]Blokhina O,Virolainen E,Fagerstedt K.Antioxidants,oxidative damage and oxygen deprivation stress:A review[J].Annals of Botany,2003,91(2):179-194
- [38]Song Y,Zhu L S,Wang J,et al.DNA damage and effects on antioxidative enzymes in earthworm(Eisenia foetida)induced by atrazine[J].Soil Biology and Biochemistry,2009,41(5):905-909
- [39]Maity S,Roy S,Chaudhury S,et al.Antioxidant responses of the earthworm Lampito mauritii exposed to Pb and Zn contaminated soil[J].Environmental Pollution,2008,151(1):1-7
- [40]Homa J,Stürzenbaum S R,Morgan A J,et al.Disrupted homeostasis in coelomocytes of Eisenia fetida and Allolobophorachlorotica exposed dermally to heavy metals[J].European Journal of Soil Biology,2007,43:S273-S280
- [41]Rhee J S,Raisuddin S,Lee K W,et al.Heat shock protein(Hsp)gene responses of the intertidal copepod Tigriopus japonicusto environmental toxicants[J].Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,2009,149(1):104-112