徐湘博;史雅娟;吕永龙;郑晓奇;

• 1:中国科学院生态环境研究中心城市与区域国家重点实验室
• 2:中国科学院大学

摘要(Abstract)：

试验采用人工土壤法,通过14 d急性毒性试验,研究了不同剂量TBBPA对赤子爱胜蚓(Eisenia fetida)生长、基因表达的影响。在14 d急性毒性试验中,随着暴露时间和浓度的增加,蚯蚓生长受到了显著抑制,未出现死亡现象。超氧化物歧化酶(SOD)的基因表达量在低浓度(50 mg·kg-1)时受到诱导上调;谷胱甘肽转移酶(GST)的基因表达量在400 mg·kg-1染毒组受到明显诱导,是对照组的8.73倍;热休克蛋白(Hsp70)的基因表达量在50、100和200 mg·kg-1染毒组被诱导上调。基因表达量的变化显示,在50、100、200 mg·kg-1浓度条件下,蚯蚓可以利用自身的抗氧化能力维持体内的动态平衡稳定,使得机体免受损伤。但是在超过400 mg·kg-1浓度后,TBBPA的毒性效应超过了机体的应对能力,使机体遭受损伤,外在特征主要表现为蚯蚓身体蜷缩、变细变硬。从生物标志物角度,基因表达量的变化对TBBPA毒性效应的指示作用较生长抑制率更为敏感。

关键词(KeyWords)： 四溴双酚A;赤子爱胜蚓;急性毒性;生长抑制率;基因表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.41271487);; 国家国际科技合作专项(No.2012DFA91150)

作者(Author): 徐湘博;史雅娟;吕永龙;郑晓奇;

Email:

DOI:

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