水源水中有机提取物生殖毒性评价和敏感检测指标的筛选Reproductive Toxicity of Drinking Source Water and Selection of Sensitive Bioassays
王晓祎;胡冠九;吴江;邬旸;史薇;韩晓冬;于红霞;
摘要(Abstract)：
以原代培养的大鼠睾丸细胞为体外毒性试验靶细胞,采用支持细胞、生精细胞、间质细胞的形态、细胞活力、胞外乳酸脱氢酶(LDH)活力、细胞早期凋亡坏死率和间质细胞睾酮分泌量5种检测指标评价了X市和L市水源水中有机提取物的生殖毒性,并筛选了对痕量有机污染物敏感的生殖毒性指标.研究结果表明,两市水源水中有机提取物皆可引起支持细胞、间质细胞和生精细胞形态的变化,相比较而言,对生精细胞的影响更严重.X市水源水有机提取物对3种生殖细胞胞外LDH活力没有显著影响(p>0.05);而在L市水源水中有机提取物的暴露下,支持细胞和间质细胞胞外LDH活力显著增强(p<0.01).X市水源水中有机提取物可使间质细胞睾酮分泌量显著减少(p<0.01);而L市水源水有机提取物对间质细胞睾酮分泌量无明显影响(p>0.05).两市水源水有机提取物均能不同程度地诱发大鼠睾丸细胞损伤,生精细胞对环境毒物是比较敏感的细胞,细胞形态、活力、LDH活力、凋亡坏死率、睾酮分泌量等生殖毒理学检测指标可用于饮用水源地水质的评价.
关键词(KeyWords)： 水源水;有机提取物;生殖毒性;敏感指标
基金项目(Foundation): 江苏省科技计划项目(No.BS2007050);; 江苏省环境监测科研基金资助项目(No.0710);; 江苏省普通高校研究生科研创新计划资助项目(No.CX07B_170z);; 南京大学研究生科研创新基金资助项目(No.2007CL11);; 污染控制与资源化研究国家重点实验室青年创新基金资助项目(No.PCRREF07002)
作者(Author): 王晓祎;胡冠九;吴江;邬旸;史薇;韩晓冬;于红霞;
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