利用EIA法快速筛查垃圾焚烧飞灰和烟气中的二恶英毒性水平Rapid Screening of Dioxin TEQ in the Flue Gas and Fly Ash from Solid Waste Incinerators by EIA
高亮;杨健;张海军;张曙光;高建东;赵亮;纪涛;杨军;李玉军;吴才玉;陈吉平;
摘要(Abstract):
基于CAPE技术公司的兔子复合克隆抗体的二恶英(PCDD/F)酶免疫分析试剂盒,建立了快速检测垃圾焚烧飞灰和烟气中PCDD/F毒性(TEQ值)的方法.试剂盒的最低检测限为3.3pg·tube-1(即3.3pgTEQ每EIA试管),线性检测范围为10~30pg·tube-1.样品经甲苯索氏抽提后过硅胶柱连接小碳柱净化处理,TCDD/F的回收率大约为50%,与原飞灰PCDD/F分布模式相比净化处理后样品中的TCDD/F含量明显降低.用净化处理后的飞灰溶液作标准溶液,绘制PCDD/F剂量-效应关系曲线,定量分析了2个飞灰样品和2个烟气样品的TEQ浓度,结果表明分析样品的TEQ实测值(HRGC/HRMS分析获得)与预测值(标准曲线计算值)的相对偏差(Rd)均小于15%,说明用该方法定量分析垃圾焚烧飞灰和烟气中PCDD/F毒性是可行的.
关键词(KeyWords): 二恶英;酶免疫分析法;高分辨气相色谱-高分辨质谱法;垃圾焚烧
基金项目(Foundation): 天津市科技发展计划项目(No.05YFGDGX09300)
作者(Author): 高亮;杨健;张海军;张曙光;高建东;赵亮;纪涛;杨军;李玉军;吴才玉;陈吉平;
Email:
DOI:
参考文献(References):
- Behnisch P A,Hosoe K,Sakai S.2001.Bioanalytical screeningmethods for dioxins and dioxin-like compounds-a review of bioassay/biomarker technology[J].Environment International,27:413-439
- Chittim B G,Bunce N J,Hu K,Tashiro C H M,Yeo B R.1994.Comparison of GC-MS with an in vitro bioassay for PCDDsand related compounds in environmental samples[J].Chemosphere,29:1783-1788
- Engwall M,Brunstr-m B,N"f C,Hjelm K.1999.Levels ofdioxin-like compounds in sewage sludge determined with a bioassaybased on EROD induction in chicken embryo liver cultures[J].Chemosphere,38:1783-1788
- Harrison R O,Carlson R E.1997.An immunoassay for TEQscreening of dioxin/furan samples:current status of assay andapplications development[J].Chemosphere,34:915-928
- Harrison R O,Eduljee G H.1999.Immunochemical analysis fordioxins-progress and prospects[J].The Science of The TotalEnvironment,239:1-18
- Harrison R,Scotti D,Clemmey T,Doncet S.2002.Implementationof EPA Method4025(Immunoassay Screening)for Dioxin SiteAssessment and Remediation[S].CAPE Technologies,Technicalreference TR-006
- Huang H,Buekens A.1995.On the mechanisms of dioxin formationin combustion processes[J].Chemosphere,31:4099-4117
- JIS(Japanese Industrial Standard),1999.Methods for Determinationof Tetra-through Octa-Chlorodibenzo-pdioxins,Tetra-through Octa-Chlorodibenzofurans and Coplanar Polychrobiphenyls in StationarySource Emissions[S].Reference number:JIS K0311:1999
- Li W,Xu Y,Wu W Z,Schramm K M,Kettrup A.2000.Anew enzyme immunoassay for TEQ screening of dioxin/furan inenvironmental samples[J].Environmental Science,21(1):69-72(in Chinese)
- Ryu J K,Choi K C,Mulholland J A.2006.Polychlorinateddibenzo-p-dioxin(PCDD)and dibenzofuran(PCDF)isomer patternsfrom municipal waste combustion:Formation mechanism fingerprints[J].Chemosphere,65:1526-1536
- SEPAC(State Environment Protection Administration of PR China),2001.Report HJ/T77-2001.Determination of PolychlorinatedDibenzo-p-dioxins and Polychlorinated Dibenzo-p-furans byIsotope Dilution HRGC/HRMS[S].Beijing:SEPAC(in Chinese)
- Schneider U A,Brown M M,Logan R A,Millar L C,Bunce NJ.1995.Screening assay for dioxin-like compounds based oncompetitive binding to the murine hepatic Ah receptor.1.Assaydevelopment[J].Environmental Science&Technology,29:2595-2602
- Wang C Z,Hu X M,Shi R,Qi G S,Li R.2006.Bioassay ofdioxin-like chemicals[J].China Safety Science Journal,16(5):135-140(in Chinese)
- Xu Y,Wu W Z,Zhang Y Y.1996.Rapid quantitative screeningof dioxin-like compounds by EROD induction bioassay[J].ChineseEnvironmental Science,16(14):279-284(in Chinese)
- Zheng M H,Yang L C,Zhang B,Xu X B.2002.Progress andprospects in analysis of dioxons[J].Journal of InternationalAnalysis,21(4):91-94(in Chinese)
- 黎雯,徐盈,吴文忠,Schramm K M,Kettrup A.2000.利用EIA生物测试法快速定量筛选环境样品中的二恶英污染物[J].环境科学,21(1):69-72
- 国家环保局.2001.HJ/T77-2001.多氯二苯并二恶英和多氯二苯并呋喃的测定——同位素稀释高分辨毛细管气相色谱/高分辨质谱法[S].北京:国家环保局
- 王承智,胡莜敏,石荣,祁国恕,李锐.2006.二恶英类物质的生物检测方法[J].中国安全科学学报,16(5):135-140
- 徐盈,吴文忠,张甬元.1996.利用EROD生物测试法快速筛选二恶英类化合物[J].中国环境科学,16(14):279-284
- 郑明辉,杨柳春,张兵,徐晓白.2002.二恶英类化合物分析研究进展[J].分析测试学报,21(4):91-94