利用EIA法快速筛查垃圾焚烧飞灰和烟气中的二恶英毒性水平Rapid Screening of Dioxin TEQ in the Flue Gas and Fly Ash from Solid Waste Incinerators by EIA
高亮;杨健;张海军;张曙光;高建东;赵亮;纪涛;杨军;李玉军;吴才玉;陈吉平;
摘要(Abstract)：
基于CAPE技术公司的兔子复合克隆抗体的二恶英(PCDD/F)酶免疫分析试剂盒,建立了快速检测垃圾焚烧飞灰和烟气中PCDD/F毒性(TEQ值)的方法.试剂盒的最低检测限为3.3pg·tube-1(即3.3pgTEQ每EIA试管),线性检测范围为10～30pg·tube-1.样品经甲苯索氏抽提后过硅胶柱连接小碳柱净化处理,TCDD/F的回收率大约为50%,与原飞灰PCDD/F分布模式相比净化处理后样品中的TCDD/F含量明显降低.用净化处理后的飞灰溶液作标准溶液,绘制PCDD/F剂量-效应关系曲线,定量分析了2个飞灰样品和2个烟气样品的TEQ浓度,结果表明分析样品的TEQ实测值(HRGC/HRMS分析获得)与预测值(标准曲线计算值)的相对偏差(Rd)均小于15%,说明用该方法定量分析垃圾焚烧飞灰和烟气中PCDD/F毒性是可行的.
关键词(KeyWords)： 二恶英;酶免疫分析法;高分辨气相色谱-高分辨质谱法;垃圾焚烧
基金项目(Foundation): 天津市科技发展计划项目(No.05YFGDGX09300)
作者(Author): 高亮;杨健;张海军;张曙光;高建东;赵亮;纪涛;杨军;李玉军;吴才玉;陈吉平;
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