李嘉伟;尹晓宇;周旖妮;杨景峰;董武;

• 1:内蒙古民族大学动物科学技术学院内蒙古自治区毒物监控及毒理学重点实验室

摘要(Abstract)：

多溴联苯醚及其羟基代谢物(OH-BDEs)被认为是内分泌干扰物,特别是对甲状腺激素调节的影响。调查并比较了BDE-99及5-OH-BDE-99对甲状腺素受体的影响。斑马鱼胚胎暴露于不同浓度的BDE-99和5-OH-BDE-99中,导致斑马鱼眼球黑色素的色素沉着减少,使用原位杂交以及定量PCR方法检测,发现THRβ在斑马鱼前脑部位表达,并有明显的降低。通过体外实验也证实BDE-99和5-OH-BDE-99能够与THRβ受体结合,尤其是5-OH-BDE-99的结合更为强烈。研究表明,BDE-99和5-OH-BDE-99对斑马鱼胚胎早期THRβ的活性有干扰作用,并导致眼部黑色素沉积减少。上述研究结果对BDE-99和5-OH-BDE-99的生物毒理学机制研究有一定的借鉴意义。

关键词(KeyWords)： PBDE;斑马鱼胚胎;色素沉着;THRβ;BDE-99;5-OH-BDE-99

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(21267015,81360508,21567019);; 内蒙古民族大学特色交叉学科群建设项目(MDXK008);内蒙古民族大学研究生科研创新项目(NMDSS1860);; 内蒙古毒物监控及毒理学重点实验室开放课题项目(MDK2019074)

作者(Author): 李嘉伟;尹晓宇;周旖妮;杨景峰;董武;

Email:

DOI:

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