五溴联苯醚(BDE-99)和羟基五溴联苯醚(5-OH-BDE-99)经由THRβ影响斑马鱼胚胎眼部色素的沉着BDE-99 and 5-OH-BDE-99 Affect the Pigmentation of the Eyes of Zebrafish Embryos via THRβ
李嘉伟;尹晓宇;周旖妮;杨景峰;董武;
摘要(Abstract):
多溴联苯醚及其羟基代谢物(OH-BDEs)被认为是内分泌干扰物,特别是对甲状腺激素调节的影响。调查并比较了BDE-99及5-OH-BDE-99对甲状腺素受体的影响。斑马鱼胚胎暴露于不同浓度的BDE-99和5-OH-BDE-99中,导致斑马鱼眼球黑色素的色素沉着减少,使用原位杂交以及定量PCR方法检测,发现THRβ在斑马鱼前脑部位表达,并有明显的降低。通过体外实验也证实BDE-99和5-OH-BDE-99能够与THRβ受体结合,尤其是5-OH-BDE-99的结合更为强烈。研究表明,BDE-99和5-OH-BDE-99对斑马鱼胚胎早期THRβ的活性有干扰作用,并导致眼部黑色素沉积减少。上述研究结果对BDE-99和5-OH-BDE-99的生物毒理学机制研究有一定的借鉴意义。
关键词(KeyWords): PBDE;斑马鱼胚胎;色素沉着;THRβ;BDE-99;5-OH-BDE-99
基金项目(Foundation): 国家自然科学基金资助项目(21267015,81360508,21567019);; 内蒙古民族大学特色交叉学科群建设项目(MDXK008);内蒙古民族大学研究生科研创新项目(NMDSS1860);; 内蒙古毒物监控及毒理学重点实验室开放课题项目(MDK2019074)
作者(Author): 李嘉伟;尹晓宇;周旖妮;杨景峰;董武;
Email:
DOI:
参考文献(References):
- [1]Zezza D,Tait S,Della Salda L,et al.Toxicological,gene expression and histopathological evaluations of environmentally realistic concentrations of polybrominated diphenyl ethers PBDE-47,PBDE-99 and PBDE-209 on zebrafish embryos[J].Ecotoxicology and Environmental Safety,2019,183:109566
- [2]Mc Donald T A.A perspective on the potential health risks of PBDEs[J].Chemosphere,2002,46(5):745-755
- [3]Giraudo M,Douville M,Letcher R J,et al.Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy) ethane and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate[J].Aquatic Toxicology,2017,186:40-49
- [4]Brown F R,Winkler J,Visita P,et al.Levels of PB-DEs,PCDDs,PCDFs,and coplanar PCBs in edible fish from California coastal waters[J].Chemosphere,2006,64(2):276-286
- [5]Rose M,Fernandes A,Mortimer D,et al.Contamination of fish in UK fresh water systems:Risk assessment for human consumption[J].Chemosphere,2015,122:183-189
- [6]Guo H,Zheng X,Ru S,et al.The leaching of additivederived flame retardants (FRs) from plastics in avian digestive fluids:The significant risk of highly lipophilic FRs[J].Journal of Environmental Sciences,2019,85:200-207
- [7]Boyles E,Nielsen C K.PBDEs and dechloranes in raccoons in the Midwestern United States[J].Bulletin of Environmental Contamination and Toxicology,2017,98(6):758-762
- [8]Chen Y P,Zheng Y J,Liu Q,et al.PBDEs (polybrominated diphenyl ethers) pose a risk to captive giant pandas[J].Environmental Pollution,2017,226:174-181
- [9]Wemken N,Drage D S,Cellarius C,et al.Emerging and legacy brominated flame retardants in the breast milk of first time Irish mothers suggest positive response to restrictions on use of HBCDD and Penta-and OctaBDE formulations[J].Environmental Reseach,2019,180:108805
- [10]Yin S,Guo F,Aamir M,et al.Multicenter biomonitoring of polybrominated diphenyl ethers (PBDEs) in colostrum from China:Body burden profile and risk assessment[J].Environmental Research,2019,179:108828
- [11]Macaulay L J,Chen A,Rock K D,et al.Developmental toxicity of the PBDE metabolite 6-OH-BDE-47 in zebrafish and the potential role of thyroid receptor beta[J].Aquatic Toxicology,2015,168:38-47
- [12]Yang J,Zhao H,Chan K M.Toxic effects of polybrominated diphenyl ethers (BDE-47 and 99) and localization of BDE-99 induced cyp1a mRNA in zebrafish larvae[J].Toxicology Reports,2017,4:614-624
- [13]Chen L,Yu K,Huang C,et al.Prenatal transfer of polybrominated diphenyl ethers (PBDEs) results in developmental neurotoxicity in zebrafish larvae[J].Environmental&Science Technology,2012,46 (17):9727-9734
- [14]Wang F,Fang M,Hinton D E,et al.Increased coiling frequency linked to apoptosis in the brain and altered thyroid signaling in zebrafish embryos (Danio rerio) exposed to the PBDE metabolite 6-OH-BDE-47[J].Chemosphere,2018,198:342-350
- [15]Linares V,Belles M,Domingo J L.Human exposure to PBDE and critical evaluation of health hazards[J].Archives of Toxicology,2015,89(3):335-356
- [16]Abolaji A O,Kamdem J P,Lugokenski T H,et al.Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster[J].Free Radical Biology and Medicine,2014,71:99-108
- [17]Balch G C,Velez-Espino L A,Sweet C,et al.Inhibition of metamorphosis in tadpoles of Xenopus laevis exposed to polybrominated diphenyl ethers (PBDEs)[J].Chemosphere,2006,64(2):328-338
- [18]Macaulay L J,Chernick M,Chen A,et al.Exposure to a PBDE/OH-BDE mixture alters juvenile zebrafish(Danio rerio) development[J].Environmental Toxicology and Chemistry,2017,36(1):36-48
- [19]Levy-Bimbot M,Major G,Courilleau D,et al.Tetrabromobisphenol-A disrupts thyroid hormone receptor alpha function in vitro:Use of fluorescence polarization to assay corepressor and coactivator peptide binding[J].Chemosphere,2012,87(7):782-788
- [20]Dong W,Macaulay L J,Kwok K W,et al.The PBDEmetabolite 6-OH-BDE-47 affects melanin pigmentation and THRβmRNA expression in the eye of zebrafish embryos[J].Endocrine Disruptors,2014,2 (1):e969072
- [21]Zheng J,Hashimoto A,Putnam M,et al.Development of a thyroid hormone receptor targeting conjugate[J].Bioconjugate Chemistry,2008,19(6):1227-1234
- [22]Benvenuti S,Luciani P,Cellai I,et al.Thyroid hormones promote cell differentiation and up-regulate the expression of the seladin-1 gene in in vitro models of human neuronal precursors[J].Journal of Endocrinology,2008,197(2):437-446
- [23]Xing W,Govoni K E,Donahue L R,et al.Genetic evidence that thyroid hormone is indispensable for prepubertal insulin-like growth factor-I expression and bone acquisition in mice[J].Journal of Bone and Mineral Research,2012,27(5):1067-1079
- [24]Helbing C C,Bailey C M,Ji L,et al.Identification of gene expression indicators for thyroid axis disruption in a Xenopus laevis metamorphosis screening assay.Part 1.Effects on the brain[J].Aquatic Toxicology,2007,82(4):227-241
- [25]Argumedo G S,Sanz C R,Olguin H J.Experimental models of developmental hypothyroidism[J].Hormone Metabolic Research,2012,44(2):79-85
- [26]Rivas M,Naranjo J R.Thyroid hormones,learning and memory[J].Genes Brain and Behavior,2007,6(1):40-44
- [27]Azadi S,Zhang Y,Caffe A R,et al.Thyroid-beta2 and the retinoid RAR-alpha,RXR-gamma and ROR-beta2receptor mRNAs;expression profiles in mouse retina,retinal explants and neocortex[J].Neuroreport,2002,13(6):745-750
- [28]Xing W,Aghajanian P,Goodluck H,et al.Thyroid hormone receptor-beta1 signaling is critically involved in regulating secondary ossification via promoting transcription of the Ihh gene in the epiphysis[J].American Journal of Physiology Endocrinology and Metabolism,2016,310(10):E846-E854
- [29]Arbogast P,Flamant F,Godement P,et al.Thyroid hormone signaling in the mouse retina[J].PLo S One,2016,11(12):e0168003
- [30]Jones I,Srinivas M,Ng L,et al.The thyroid hormone receptor beta gene:Structure and functions in the brain and sensory systems[J].Thyroid,2003,13 (11):1057-1068
- [31]Carlsson G,Kulkarni P,Larsson P,et al.Distribution of BDE-99 and effects on metamorphosis of BDE-99 and-47 after oral exposure in Xenopus tropicalis[J].Aquatic Toxicology,2007,84(1):71-79
- [32]Wu L,Li Y,Ru H,et al.Parental exposure to 2,2’,4,4’5-pentain polybrominated diphenyl ethers (BDE-99) causes thyroid disruption and developmental toxicity in zebrafish[J].Toxicology Applied Pharmacology,2019,372:11-18