微纳尺度SiO_2对雄性大鼠生殖功能损伤的实验研究Experimental Study on the Reproductive Damage of Male Rats Induced by Micro-nano-scale SiO_2
林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;
摘要(Abstract)：
为了探讨纳米与微米尺度SiO2对雄性大鼠的生殖毒性作用,选择不同剂量的纳米SiO2(20～40nm)与微米SiO2(1～10μm)采用气管滴注方式对雄性Wistar大鼠分组染毒.于染毒5周后处死大鼠,检查附睾精子形态,并检测睾丸组织和血清中睾丸功能标志酶活性变化以及性激素含量的变化.结果表明:1)高、低剂量的纳米和微米SiO2染毒均可使大鼠发生程度不同的精子数量减少、精子活动率降低、精子畸形率升高;2)纳米SiO2染毒可使大鼠睾丸组织SDH、LDH和血清中ACP活性显著降低,而微米SiO2染毒对这些指标的影响不显著;3)纳米SiO2和高剂量微米SiO2染毒可使大鼠血清T和睾丸匀浆T浓度显著降低,而对血清LH没有显著影响;4)与微米SiO2相比,纳米SiO2对大鼠生殖功能的损伤有更严重的趋势,但相同剂量下,纳米SiO2和微米SiO2相比,各指标均无显著性差异.以上结果表明,微米和纳米尺度SiO2染毒均可使大鼠生殖功能产生损伤,使部分生殖功能指标发生显著变化;与微米SiO2相比,纳米SiO2对大鼠生殖功能的损伤有更严重的趋势.
关键词(KeyWords)： 纳米SiO_2;微米SiO_2;雄性大鼠;生殖损伤
基金项目(Foundation): 国家自然科学基金项目(No.90406024);; 国家高技术研究发展规划(863)项目(No.2006AA03Z330)
作者(Author): 林本成;袭著革;张英鸽;张华山;杨丹凤;孙欣;张伟;刘焕亮;
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