0,0-二甲基-(2,2,2-三氯-1-羟基乙基)磷酸酯对4种藻类生长的影响Toxic Effects of 0,0-Two-( 2,2,2-Three Chloro-1-Hydroxy Ethyl) Phosphate on Four Kinds of Algae Growth
景江;夏林军;吴菊珍;孙磊;
摘要(Abstract):
0,0-二甲基-(2,2,2-三氯~(-1)-羟基乙基)磷酸酯(敌百虫)为广谱杀虫剂,用途广泛,但对藻类的毒理学效应研究还有待完善。采用4种受试藻样,设置5个敌百虫浓度组(1、5、10、50和100 mg·L~(-1))和对照组,实验周期40 d,藻细胞的初始接种密度106cells·m L~(-1),光暗比12 h/12 h,24 h曝气,24 h磁力搅拌,实验温度25℃,p H 6.8,每隔24 h取样。结果表明:5 mg·L~(-1)、10 mg·L~(-1)、50 mg·L~(-1)浓度的敌百虫对铜绿微囊藻和小球藻的生长有促进作用,其中以50 mg·L~(-1)浓度组的促进作用最为显著,促进作用主要表现在生长峰值的延后以及生长对数期的延长,而高剂量(100 mg·L~(-1))的敌百虫则有抑制藻生长的作用。取50 mg·L~(-1)敌百虫浓度组以及铜绿微囊藻和小球藻作进一步深入研究,结果表明:50 mg·L~(-1)敌百虫浓度组的叶绿素a含量峰值比对照组高30%,细胞体内的SOD、ATP含量都高于对照组。敌百虫的使用浓度通常在0.1~1.0 mg·L~(-1),低于本实验最佳浓度。本实验中1 mg·L~(-1)敌百虫对藻生长影响效果不明显。
关键词(KeyWords): 敌百虫;铜绿微囊藻;小球藻;细胞密度;叶绿素a;SOD;ATP
基金项目(Foundation): 四川省科技厅重点项目(2011ZR0139);; 四川省教育厅项目(15ZB0309)
作者(Author): 景江;夏林军;吴菊珍;孙磊;
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