张杏丽;邹威;周启星;

• 1:河南师范大学环境学院黄淮水环境与污染防治教育部重点实验室河南省环境污染控制重点实验室
• 2:南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室天津市城市生态环境修复与污染防治重点实验室

摘要(Abstract)：

磷酸三苯酯(TPhP)是广泛存在于环境介质和生物体内的一种典型有机磷阻燃剂。为探求TPhP诱发水生动物发育毒性的分子机制,本研究以斑马鱼为模式动物,将发育至2.5 hpf (hours post fertilization)的斑马鱼胚胎暴露于0.0025、0.1、1、10、100和1 000μg·L~(-1)TPhP溶液至7 dpf (days post fertilization),考察斑马鱼胚胎生长发育指标和线粒体功能的变化,通过代谢组学分析揭示相关分子机制。结果表明,环境相关浓度(0.0025、0.1和1μg·L~(-1)) TPhP对斑马鱼胚胎发育无显著影响,但是轻微干扰了斑马鱼的代谢过程。100和1 000μg·L~(-1)TPhP暴露引起斑马鱼心跳速率、孵化率和线粒体膜电位明显下调,畸形率分别增加6.8倍和12.5倍,死亡率分别增加7.2倍和16.5倍。代谢组学分析发现,10、100和1 000μg·L~(-1)TPhP显著抑制斑马鱼氨基酸代谢,降低缬氨酸、亮氨酸和异亮氨酸水平,抑制氨酰-tRNA生物合成过程;同时引起葡萄糖糖酵解过程和三羧酸循环发生障碍。氨基酸和糖代谢异常可能是TPhP引起斑马鱼发育畸形的主要原因,线粒体功能紊乱可能是TPhP诱发三羧酸循环障碍的原因。上述研究结果为TPhP发育毒性机制分析提供了新思路。

关键词(KeyWords)： 磷酸三苯酯;斑马鱼;发育毒性;代谢组学

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31670508);; 博士科研启动课题(5101219170132);; 博士后科研经费(5101219470216)

作者(Author): 张杏丽;邹威;周启星;

Email:

DOI:

参考文献(References)：

• [1] Zhang W W,Wang P,Li Y M,et al. Spatial and temporal distribution of organophosphate esters in the atmosphere of the Beijing-Tianjin-Hebei Region,China[J].Environmental Pollution,2019,244:182-189
• [2] Kim U J,Kannan K. Occurrence and distribution of organophosphate flame retardants/plasticizers in surface waters,tap water,and rainwater:Implications for human exposure[J]. Environmental Science&Technology,2018,52(10):5625-5633
• [3] He C,Wang X Y,Thai P,et al. Organophosphate and brominated flame retardants in Australian indoor environments:Levels,sources,and preliminary assessment of human exposure[J]. Environmental Pollution,2018,235:670-679
• [4] Yadav I C,Devi N L,Li J,et al. Organophosphate ester flame retardants in Nepalese soil:Spatial distribution,source apportionment and air-soil exchange assessment[J]. Chemosphere,2018,190:114-123
• [5] Mo L,Zheng J,Wang T,et al. Legacy and emerging contaminants in coastal surface sediments around Hainan Island in South China[J]. Chemosphere,2019,215:133-141
• [6]周启星，赵梦阳，来子阳，等．有机磷阻燃剂的环境暴露与动物毒性效应[J]．生态毒理学报，2017,12(5):1-11Zhou Q X,Zhao M Y,Lai Z Y,et al. Researching progress in toxic effects of organophosphorus flame retardants released into the environment[J]. Asian Journal of Ecotoxicology,2017,12(5):1-11(in Chinese)
• [7] Ospina M,Jayatilaka N K,Wong L Y,et al. Exposure to organophosphate flame retardant chemicals in the U.S.general population:Data from the 2013-2014 National Health and Nutrition Examination Survey[J]. Environment International,2018,110:32-41
• [8] Li P,Jin J,Wang Y,et al. Concentrations of organophosphorus,polybromobenzene,and polybrominated diphenyl ether flame retardants in human serum,and relationships between concentrations and donor ages[J].Chemosphere,2017,171:654-660
• [9]ˇCechováE,Vojta，Kukuˇc ka P,et al. Legacy and alternative halogenated flame retardants in human milk in Europe:Implications for childrens health[J]. Environment International,2017,108:137-145
• [10] Alves A,Covaci A,Voorspoels S.Method development for assessing the human exposure to organophosphate flame retardants in hair and nails[J]. Chemosphere,2017,168:692-698
• [11] Zhang X L,Zou W,Mu L,et al. Rice ingestion is a major pathway for human exposure to organophosphate flame retardants(OPFRs)in China[J]. Journal of Hazardous Materials,2016,318:686-693
• [12] Poma G,Sales C,Bruyland B,et al. Occurrence of organophosphorus flame retardants and plasticizers(PFRs)in Belgian foodstuff and estimation of the dietary exposure of the adult population[J]. Environmental Science&Technology,2018,52(4):2331-2338
• [13] Wang R M,Tang J H,Xie Z Y,et al. Occurrence and spatial distribution of organophosphate ester flame retardants and plasticizers in 40 rivers draining into the Bohai Sea,North China[J]. Environmental Pollution,2015,198:172-178
• [14] Wang X W,Liu J F,Yin Y G. Development of an ultrahigh-performance liquid chromatography-tandem mass spectrometry method for high throughput determination of organophosphorus flame retardants in environmental water[J]. Journal of Chromatography A,2011,1218(38):6705-6711
• [15] Li J,Yu N Y,Zhang B B,et al. Occurrence of organophosphate flame retardants in drinking water from China[J]. Water Research,2014,54:53-61
• [16] Su G,Crump D,Letcher R J,et al. Rapid in vitro metabolism of the flame retardant triphenyl phosphate and effects on cytotoxicity and mRNA expression in chicken embryonic hepatocytes[J]. Environmental Science&Technology,2014,48(22):13511-13519
• [17] Yuan S,Li H,Dang Y,et al. Effects of triphenyl phosphate on growth,reproduction and transcription of genes of Daphnia magna[J]. Aquatic Toxicology, 2018,195:58-66
• [18] Liu X,Cai Y,Wang Y,et al. Effects of tris(1,3-dichloro-2-propyl)phosphate(TDCPP)and triphenyl phosphate(TPP)on sex-dependent alterations of thyroid hormones in adult zebrafish[J]. Ecotoxicology and Environmental Safety,2019,170:25-32
• [19] Shi Q,Wang M,Shi F,et al. Developmental neurotoxicity of triphenyl phosphate in zebrafish larvae[J]. Aquatic Toxicology,2018,203:80-87
• [20] Li Y,Wang C,Zhao F R,et al. Environmentally relevant concentrations of the organophosphorus flame retardant triphenyl phosphate(TPhP)impaired testicular development and reproductive behaviors in Japanese Medaka(Oryzias latipes)[J]. Environmental Science&Technology Letters,2018,54:649-654
• [21] Hong X S,Chen R,Hou R,et al. Triphenyl phosphate(TPHP)-induced neurotoxicity in adult male Chinese rare minnows(Gobiocypris rarus)[J]. Environmental Science&Technology,2018,52:11895-11903
• [22] Isales G M,Hipszer R A,Raftery T D,et al. Triphenyl phosphate induced developmental toxicity in zebrafish:Potential role of the retinoic acid receptor[J]. Aquatic Toxicology,2015,161:221-230
• [23]耿柠波，张海军，王菲迪，等．代谢组学技术在环境毒理学研究中的应用[J]．生态毒理学报，2016,11(3):26-35Geng N B,Zhang H J,Wang F D,et al. A review on the application of metabonomic approaches in environmental toxicology[J]. Asian Journal of Ecotoxicology,2016,11(3):26-35(in Chinese)
• [24] Zhang X L,Zhou Q X,Zou W,et al. Molecular mechanisms of developmental toxicity induced by graphene oxide at predicted environmental concentrations[J]. Environmental Science&Technology,2017,51:7861-7871
• [25] Xu Y,Wang W,Zhou J,et al. Metabolomics analysis of a mouse model for chronic exposure to ambient PM2.5[J]. Environmental Pollution,2019,274:953-963
• [26] Song Y,Li R,Zhang Y,et al. Mass spectrometry-based metabolomics reveals the mechanism of ambient fine particulate matter and its components on energy metabolic reprogramming in BEAS-2B cells[J]. Science of the Total Environment,2019,651:3139-3150
• [27] Li X,Mu L,Hu X. Integrating proteomics,metabolomics and typical analysis to investigate the uptake and oxidative stress of graphene oxide and polycyclic aromatic hydrocarbons[J]. Environmental Science:Nano,2018,5(1):115-129
• [28]高丹，同帜，张圣虎，等．4种典型有机磷阻燃剂对斑马鱼胚胎毒性及风险评价[J]．生态与农村环境学报，2017,33(9):836-844Gao D,Tong Z,Zhang S H,et al. Toxicity of four typical organic phosphorus flame retardants to zebrafish embryo and risk assessment[J]. Journal of Ecology and Rural Environment,2017,33(9):836-844(in Chinese)
• [29] Shi Q P,Wang M,Shi F Q,et al. Developmental neurotoxicity of triphenyl phosphate in zebrafish larvae[J].Aquatic Toxicology,2018,203:80-87
• [30] Yan L,Gong C X,Zhang X F,et al. Perturbation of metabonome of embryo/larvae zebrafish after exposure to fipronil[J]. Environmental Toxicology&Pharmacology,2016,48:39-45
• [31] Wang D H,Jackson J R,Twining C,et al. Saturated branched chain,normal odd-carbon-numbered,and N-3(Omega-3)polyunsaturated fatty acids in freshwater fish in the Northeastern United States[J]. Journal of Agricultural Food and Chemistry,2016,64(40):7512-7519
• [32] Xu N N,Mu P Q,Yin Z Q,et al. Analysis of the enantioselective effects of PCB95 in zebrafish(Danio rerio)embryos through targeted metabolomics by UPLC-MS/MS[J]. PloS One,2016,11(8):e0160584
• [33] Wentzel P,Wentzel C R,Greskog M B,et al. Induction of embryonic dysmorphogenesis by high glucose concentration,disturbed inositol metabolism,and inhibited protein kinase C activity[J]. Teratology,2001,63(5):193-201