毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;

• 1:华中师范大学生命科学学院环境科学实验室和遗传调控与整合生物学湖北省重点实验室
• 2:军事医学科学院卫生和环境医学研究所环境毒理学研究室

摘要(Abstract)：

为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物(Nano-MnO2)和常规尺度二氧化锰颗粒物(Nor-MnO2)所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度(0、100、200、400μg·mL-1)的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明:与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比(TailDNA%)和尾矩(TailMoment)显著增加(p<0.01);而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重(p<0.01).结果提示:二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常规尺度二氧化锰颗粒毒作用更强烈.

关键词(KeyWords)： 纳米尺度二氧化锰颗粒物;常规尺度二氧化锰颗粒物;DNA损伤;彗星实验;Hela细胞

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基金项目(Foundation): the Grant of National High-Tech R&D Program (863 Program)(No. 2006AA03Z330);; the Grants of China National Key Technology R&D Programs (No. 2006BAI19B05; No. 2006BAJ02A10)

作者(Author): 毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;

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