二氧化锰颗粒对Hela细胞DNA损伤的尺度依赖性毒作用(英文)Size-Dependent Toxicity of Dioxide Manganese Particles on DNA Damage in Hela Cells
毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;
摘要(Abstract):
为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物(Nano-MnO2)和常规尺度二氧化锰颗粒物(Nor-MnO2)所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度(0、100、200、400μg·mL-1)的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明:与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比(TailDNA%)和尾矩(TailMoment)显著增加(p<0.01);而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重(p<0.01).结果提示:二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常规尺度二氧化锰颗粒毒作用更强烈.
关键词(KeyWords): 纳米尺度二氧化锰颗粒物;常规尺度二氧化锰颗粒物;DNA损伤;彗星实验;Hela细胞
基金项目(Foundation): the Grant of National High-Tech R&D Program (863 Program)(No. 2006AA03Z330);; the Grants of China National Key Technology R&D Programs (No. 2006BAI19B05; No. 2006BAJ02A10)
作者(Author): 毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;
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参考文献(References):
- Ferin J, Oberdster G, Penny D P. 1992. Pulmonary retention ofultrafine and fine particles in rats [J]. American Journal ofRespiratory Cell and Molecular Biology, 6: 535-542
- Hartog J J, Hoek G, Peters A, Timonen K L, Ibald -Mulli A,Brunekreef B, Heinrich J, Tiittanen P, Van Wijnene J H,Kreyling W, Kulmala M, Pekkanen J. 2003. Effects of fine andultrafine particles on cardiorespiratory symptoms in elderly subjectswith coronary heart diseas [J]. American Journal of Epidemiology,157: 613-623
- Nel A, Xia T, Mdler L , Li N. 2006. Toxic potential ofmaterials at the nanolevel [J]. Science, 311: 622-627
- Oberdrster G, Finkelstein J N, Johnston C, Gelein R, Cox C,Baggs R, Elder A C. 2000. Acute pulmonary effects of ultrafineparticles in rats and mice [J]. Research Report (Health EffectsInstitute), 96: 5-74; disc, 75-86
- Papageorgiou I, Brown C, Schins R, Singh S, Newson R, DavisS, Fisher J, Ingham E, Case C P. 2007. The effect of nano -and micron -sized particles of cobalt -chromium alloy on humanfibroblasts in vitro [J]. Biomaterials, 28: 2946-2958
- Tice R R, Agurell E, Anderson D, Burlinson B, Hartmann A,Kobayashi H, Miyamae Y, Rojas E, Ryu J C, Sasaki Y F.2000. Single cell gel/comet assay: Guidelines for in vitro and invivo genetic toxicology testing [J]. Environmental and MolecularMutagenesis, 35: 206-221
- Wang B, Feng W Y, Zhao Y L, Chai Z F, Wang H F, Jia G.2004. Research progression in biological effect of nanomaterialsand its toxicology[J]. Science in China(Ser. B Chemisty), 35:1-9 (in Chinese)
- Wu K, Yang G T, Lou X H, Wang L M, Li Y, Ke X H,Yang X. 2006. DNA -protein crosslinks and DNA damages ofmice lung induced by formaldehyde[J]. Journal of Public Healthand Preventive Medicine, 17: 15-18 (in Chinese)
- Yang Y J, Huang C D. 2006. Preparation of nano -structuredMnO2 and its application in chemical power sources [J]. ChemicalIndustry and Engineering Progress, 24: 383-387 (in Chinese)
- Ying X Q, Zeng Q L, Zhu H J, Zheng Y F, Zhu X Q. 2006.Nanosized dioxide and normal silicon dioxide induced acutepulmonary damage in rat [J]. Chinese Journal of IndustrialHygiene and Occupational, 24: 116-117 (in Chinese)
- Zhang Q W, Kusaka Y, Donaldson K. 2000. Comparativepulmonary responses caused by exposure to standard cobalt andultrafine cobalt[J]. Journal of Occupational Health, 42: 179-184