二氧化锰颗粒对Hela细胞DNA损伤的尺度依赖性毒作用(英文)Size-Dependent Toxicity of Dioxide Manganese Particles on DNA Damage in Hela Cells
毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;
摘要(Abstract)：
为了观察二氧化锰颗粒物所致的尺度依赖性DNA损伤作用,将纳米尺度二氧化锰颗粒物(Nano-MnO2)和常规尺度二氧化锰颗粒物(Nor-MnO2)所致的DNA损伤进行了对比研究.将Hela细胞分别暴露于不同浓度(0、100、200、400μg·mL-1)的Nano-MnO2和Nor-MnO2中,染毒24h,采用彗星实验检测Hela细胞的DNA损伤水平.结果表明:与对照组相比,Nano-MnO2和Nor-MnO2均可使彗尾DNA百分比(TailDNA%)和尾矩(TailMoment)显著增加(p<0.01);而在同一浓度水平上,Nano-MnO2所致的DNA损伤则比Nor-MnO2所致的DNA损伤更为严重(p<0.01).结果提示:二氧化锰颗粒对Hela细胞DNA损伤具有尺度依赖性毒作用,纳米尺度比常规尺度二氧化锰颗粒毒作用更强烈.
关键词(KeyWords)： 纳米尺度二氧化锰颗粒物;常规尺度二氧化锰颗粒物;DNA损伤;彗星实验;Hela细胞
基金项目(Foundation): the Grant of National High-Tech R&D Program (863 Program)(No. 2006AA03Z330);; the Grants of China National Key Technology R&D Programs (No. 2006BAI19B05; No. 2006BAJ02A10)
作者(Author): 毛彩霞;杨光涛;乔永康;李岩;袭著革;杨旭;
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