路阳;卢春凤;苑晓燕;李利忠;王以美;赵君;彭双清;

• 1:军事医学科学院 疾病预防控制所毒理学评价研究中心
• 2:佳木斯大学

摘要(Abstract)：

为了研究铅与纳米SiO2联合染毒所致的细胞损伤特征,并从氧化应激方面探讨其可能的作用机制。用铅和SiO2处理A549细胞,采用四唑盐(MTT)比色法检测细胞存活率,评价铅和SiO2联合染毒所致的细胞损伤特征;采用硫代巴比妥酸(TBA)比色法检测细胞内丙二醛(MDA)含量,评价铅与SiO2联合染毒所致细胞的氧化应激状态;检测了细胞内抗氧化物还原型谷胱甘肽(GSH)含量以及细胞内抗氧化酶的活性,以评价铅与SiO2联合染毒对细胞抗氧化系统的影响。将实验数据进行ANOVA分析。结果表明,铅、SiO2单独染毒组各指标没有明显改变;而联合染毒能造成细胞氧化损伤,表现为细胞存活率、GSH水平、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性显著低于对照组及2个单独染毒组(P<0.05),细胞内MDA含量显著高于对照组及各单独染毒组(P<0.05)。可见,联合染毒可引起明显的细胞毒性,氧化损伤可能是铅与SiO联合染毒致肺细胞毒性损伤的作用机制之一。

关键词(KeyWords)： 铅;纳米SiO2;氧化损伤;联合染毒

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划项目(2011CB503803)

作者(Author): 路阳;卢春凤;苑晓燕;李利忠;王以美;赵君;彭双清;

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DOI:

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