稀有鮈鲫神经发育相关基因的克隆、同源性分析及组织分布Cloning,Homology Analysis,and Distribution of Neural Development Related Genes of Chinese Rare Minnow( Gobiocypris rarus)
陈曦;洪响生;吴慧敏;查金苗;覃剑晖;
摘要(Abstract):
作为重要的本土模式鱼类,稀有鮈鲫神经发育相关的生物学背景几乎空白,导致其在神经毒性评价方面的应用受到限制。本文从稀有鮈鲫脑部克隆得到了神经发育相关的神经生长因子(nerve growth factor;ngf)、脑源性神经营养因子(brain-derived neurotrophic factor;bdnf)、胶质纤维酸性蛋白(glial fibrillary acidic protein;gfap)、髓鞘相关糖蛋白(myelin-associated glycoprotein;mag)、P0蛋白(myelin protein zero;mpz)、微管蛋白α1(α1-tubulin)和Y染色体性别决定基因10(SRY-box containing gene 10;sox10)等基因的片段,并对其进化树及表达谱进行了分析。序列分析表明,bdnf的核苷酸序列与鲫鱼、鲤鱼的同源性最高,均为98%;gfap、ngf、mag和α1-tubulin与斑马鱼的同源性最高,分别为95%、92%、92%和96%;mpz与黑头软口鲦的同源性最高,为94%;sox10与鳙鱼同源性最高,达97%。表达谱分析结果表明,ngf、bdnf、mag、mpz、α1-tubulin和sox10基因均在脑组织中表达量最高,gfap在脊髓中表达量最高,上述结果为这一模式鱼类在神经发育学和神经毒性效应评价方面的应用奠定了基础。
关键词(KeyWords): 神经系统发育相关基因;稀有鮈鲫;克隆;同源性分析;组织分布
基金项目(Foundation):
作者(Author): 陈曦;洪响生;吴慧敏;查金苗;覃剑晖;
Email:
DOI:
参考文献(References):
- [1]Cowden J,Padnos B,Hunter D,et al.Developmental exposure to valproate and ethanol alters locomotor activity and retino-tectal projection area in zebrafish embryos[J].Reproductive Toxicology,2012,33(2):165-173
- [2]Selderslaghs I W,Hooyberghs J,Blust R,et al.Assessment of the developmental neurotoxicity of compounds by measuring locomotor activity in zebrafish embryos and larvae[J].Neurotoxicology and Teratology,2013,37:44-56
- [3]Cunha C,Brambilla R,Thomas K L.A simple role for BDNF in learning and memory?[J].Frontiers in Molecular Neuroscience,2010,3(1):1-14
- [4]Park H,Poo M M.Neurotrophin regulation of neral circuitdevelopment and function[J].Nature Reviews Neuroscience,2013,14(1):7-23
- [5]Gomes F C,Garcia-Abreu J,Galou M,et al.Neurons induce GFAP gene promoter of cultured astrocytes from transgenic mice[J].Glia,1999,26(2):97-108
- [6]Nielsen A L,Jorgensen A L.Structural and functional characterization of the zebrafish gene for glial fibrillary acidic protein,GFAP[J].Gene,2003,310:123-132
- [7]Arquint M,Roder J,Chia L S,et al.Molecular cloning and primary structure of myelin-associated glycoprotein[J].Proceedings of the National Academy of Sciences of the United States of America,1987,84(2):600-604
- [8]Zhao L,Liu Q J,Chen H,et al.The effect of 2,5-hexanedione on myelin protein zero expression,and its mitigation using ginkgo biloba extract[J].Biomedical and Environmental Sciences,2011,24(4):374-382
- [9]Kelsh R N.Sorting out Sox10 functions in neural crest development[J].Bio Essays,2006,28(8):788-798
- [10]Fan C Y,Cowden J,Simmons S O,et al.Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening[J].Neurotoxicology and Teratology,2010,32(1):91-98
- [11]Bernardos R L,Raymond P A.GFAP transgenic zebrafish[J].Gene Expression Patterns,2006,6(8):1007-1013
- [12]Betancourt A M,Burgess S C,Carr R L.Effect of developmental exposure to chlorpyrifos on the expression of neurotrophin growth factors and cell-specific markers in neonatal rat brain[J].Toxicological Sciences,2006,92(2):500-506
- [13]Capiotti K M,Menezes F P,Nazario L R,et al.Early exposure to caffeine affects gene expression of adenosine receptors,DARPP-32 and BDNF without affecting sensibility and morphology of developing zebrafish(Danio rerio)[J].Neurotoxicology and Teratology,2011,33(6):680-685
- [14]Wang X Y.The exposure to nicotine affects expression of brain-derived neurotrophic factor(BDNF)and nerve growth factor(NGF)in neonate rats[J].Neurological Sciences,2015,36(2):289-295
- [15]Liang X F,Wang M,Chen X,et al.Endocrine disrupting effects of benzotriazole in rare minnow(Gobiocypris rarus)in a sex-dependent manner[J].Chemosphere,2014,112:154-162
- [16]曹文宣,王剑伟.稀有鮈鲫—一种新的鱼类实验动物[J].实验动物科学与管理,2003,20(S1):96-99Cao W X,Wang J W.Rare minnow:A new laboratory animal in China[J].Laboratory Animal Science and Management,2003,20(S1):96-99(in Chinese)
- [17]Cohen S,Levi-Montalcini R,Hamburger V.A nerve growth-stimulating factor isolated from sarcom as 37 and180[J].Proceedings of the National Academy of Sciences of the United States of America,1954,40(10):1014-1018
- [18]Scott J,Selby M,Urdea M,et al.Isolation and nucleotide sequence of a c DNA encoding the precursor of mouse nerve growth factor[J].Nature,1983,302(5908):538-540
- [19]Leibrock J L F,Hohn A,Hofer M,et al.Molecular cloning and expression of brain-drived neurotrophic factor[J].Nature,1989,341:149-152
- [20]余云开,华仲慰,郭建荣,等.人及猪脑源性神经营养因子基因克隆[J].科学通报,1994,39(1):76-78Yu Y K,Hua Z W,Guo J R,et al.Cloning of brain-derived neurotrophic factor gene of human and pigs[J].Chinese Science Bulletin,1994,39(1):76-78(in Chinese)
- [21]曹玫,杨玉华,张义正.大凉疣螈脑源性神经营养因子(BDNF)基因的克隆与序列分析[J].应用与环境生物学报,2002,8(3):314-316Cao M,Yang Y H,Zhang Y Z.Cloning and sequence analysis of brain-derived neurotrophic factor gene of Tylototriton taliangensis[J].Chinese Journal of Applied and Environmental Biology,2002,8(3):314-316(in Chinese)
- [22]杨光,陈红卫,杨玉华,等.四川山鹧鸪脑源性神经营养因子(BDNF)基因的扩增与序列分析[J].四川大学学报:自然科学版,2000,37:195-198Yang G,Chen H W,Yang Y H,et al.The amplification and sequence analysis of the brain derived neurotophic factor(BDNF)gene form Sichuan Partridge[J].Journal of Sichuan University:Natural Science Edition,2000,37:195-198(in Chinese)
- [23]林峰,杨玉华,张义正,等.小熊猫脑源性神经营养因子(BDNF)基因的克隆与序列分析[J].四川大学学报:自然科学版,1997,34(3):367-370Lin F,Yang Y H,Zhang Y Z,et al.Molecular cloning and sequencing of the brain derived neurotrophic factor(BDNF)gene from lesser panda[J].Journal of Sichuan University:Natural Science Edition,1997,34(3):367-370(in Chinese)
- [24]林峰,杨玉华,张义正,等.马来熊脑源性神经营养因子基因编码区的克隆与序列分析[J].动物学报,1998,44(1):102-106Lin F,Yang Y H,Zhang Y Z,et al.Molecular cloning and sequence of the coding region of brain derived neurotrophic factor(BDNF)gene from Helarctos malayanus[J].Acta Zoologica Sinica,1998,44(1):102-106(in Chinese)
- [25]Rehberg S,Lischka P,Glaser G,et al.Sox10 is an active nucleocytoplasmic shuttle protein,and shuttling is crucialfor sox10-mediated transactivation[J].Molecular and Cellular Biology,2002,22(16):5826-5834
- [26]章卓,苏建明,肖调义.Sox10在感觉神经元发育中的作用[J].中南林业科技大学学报,2010,30(10):94-106Zhang Z,Su J M,Xiao T Y.A genetic investigation of the role of sox10 in sensory neuron development[J].Journal of Central South University of Forestry&Technology,2010,30(10):94-106(in Chinese)
- [27]Bigini P,Bastone A,Mennini T.Glutamate transporters in the spinal cord of the wobbler mouse[J].Neuroreport,2001,12(9):1815-1820
- [28]Romero-Alemán M M,Monz'on-Mayor M,Yanes C,et al.Radial glial cells,proliferating periventricular cells,and microglia might contribute to successful structural repair in the cerebral cortex of the lizard Gallotia galloti[J].Experimental Neurology,2004,188(1):74-85
- [29]Sassoe P M,Panzanelli P,Artero C,et al.Comparative study of glial fibrillary acidic protein(GFAP)-like immunoreactivity in the retina of some representative vertebrates[J].European Journal of Histochemistry,1992,36(4):467-477
- [30]高德宏.多疣壁虎GFAP基因的克隆及其脊髓横断损伤后GFAP的表达变化[D].武汉:华中科技大学,2010Gao D H.The molecular cloning of glial fibrillary acidic protein in Gekko japonicus and its expression changes after spinal cord transection[D].Huazhong University of Science and Technology,2010(in Chinese)
- [31]李犇,李秀雯,宋默识,等.神经生长因子及其受体在雌性哺乳动物生殖系统中的作用[J].科技咨询,2009,3:9-10Li B,Li X W,Song M S,et al.The effects of nerve growth factor and its receptors in female mammalian reproductive system[J].Science&Technology Information,2009,3:9-10(in Chinese)
- [32]Ren L,Mohamed S M,Weng Q,et al.Immunolocalization of nerve growth factor(NGF)and its receptors(Trk A and p75LNGFR)in the reproductive organs of Shiba goats[J].The Journal of Reproduction and Development,2005,51(3):399-404
- [33]Shi Z.Studies on expression and regulation of nerve growth factor and inhibin/activin in reproductive organs of the female golden hamster[D].Tokyo:Tokyo University of Agricuture and Technology,2004