硝酸铊对浮萍(Lemna gibba L.)的生长抑制及其氧自由基损伤作用Growth Inhibition of Thallium( I)- nitrate on Duckweed( Lemna gibba L. )with Relation to Its Oxidative Stress
刘涛;邓红梅;王耀龙;陈永亨;陈敏婵;巫贵明;曾冬媚;杜建莹;顾颖诗;许旭超;
摘要(Abstract):
为了探讨硝酸铊(Tl(I)-nitrate)对浮萍(Lemna gibba L.)的生长抑制作用及其可能的氧化损伤机制,设置了Tl(I)-nitrate的8个处理组(0.005、0.01、0.02、0.04、0.08、0.1、0.5和1.0 mg·L-1)和对照组(0.0 mg·L-1),进行96 h急性毒性实验,测定96 h内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及丙二醛含量。结果表明:96 h暴露时间下,浮萍生长受抑制程度与Tl(I)浓度之间呈正相关,Tl(I)-nitrate对浮萍的96 h EC50为0.076 mg·L-1,NOEC为0.01 mg·L-1。CAT活性随着Tl(I)-nitrate浓度升高逐渐降低,在0.5和1 mg·L-1时,有极显著差异(p<0.01)产生,SOD活性在0.1 mg·L-1时显著升高(>46%),POD活性和MDA含量在Tl(I)浓度0.5 mg·L-1时明显上升。当Tl(I)-nitrate胁迫超出浮萍抗氧化酶系统清除活性氧的能力时,活性氧积累导致浮萍受到不可逆转的氧化损伤。Tl(I)-nitrate对浮萍生长产生明显的抑制作用
关键词(KeyWords): Tl(I);浮萍;抗氧化酶;丙二醛
基金项目(Foundation): 国家自然科学基金资助项目(No.41170399);; 国家级大学生创新训练项目(No.201211078017)
作者(Author): 刘涛;邓红梅;王耀龙;陈永亨;陈敏婵;巫贵明;曾冬媚;杜建莹;顾颖诗;许旭超;
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