刘涛;邓红梅;王耀龙;陈永亨;陈敏婵;巫贵明;曾冬媚;杜建莹;顾颖诗;许旭超;

• 1:广州大学土木工程学院
• 2:广州大学环境科学与工程学院
• 3:华南师范大学化学与环境学院

摘要(Abstract)：

为了探讨硝酸铊(Tl(I)-nitrate)对浮萍(Lemna gibba L.)的生长抑制作用及其可能的氧化损伤机制,设置了Tl(I)-nitrate的8个处理组(0.005、0.01、0.02、0.04、0.08、0.1、0.5和1.0 mg·L-1)和对照组(0.0 mg·L-1),进行96 h急性毒性实验,测定96 h内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及丙二醛含量。结果表明:96 h暴露时间下,浮萍生长受抑制程度与Tl(I)浓度之间呈正相关,Tl(I)-nitrate对浮萍的96 h EC50为0.076 mg·L-1,NOEC为0.01 mg·L-1。CAT活性随着Tl(I)-nitrate浓度升高逐渐降低,在0.5和1 mg·L-1时,有极显著差异(p<0.01)产生,SOD活性在0.1 mg·L-1时显著升高(>46%),POD活性和MDA含量在Tl(I)浓度0.5 mg·L-1时明显上升。当Tl(I)-nitrate胁迫超出浮萍抗氧化酶系统清除活性氧的能力时,活性氧积累导致浮萍受到不可逆转的氧化损伤。Tl(I)-nitrate对浮萍生长产生明显的抑制作用

关键词(KeyWords)： Tl(I);浮萍;抗氧化酶;丙二醛

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.41170399);; 国家级大学生创新训练项目(No.201211078017)

作者(Author): 刘涛;邓红梅;王耀龙;陈永亨;陈敏婵;巫贵明;曾冬媚;杜建莹;顾颖诗;许旭超;

Email:

参考文献(References)：

• [1]万顺利,马明海,徐圣友,等.水体中铊的污染治理技术研究进展[J].水处理技术,2014,40(2):15-19Wan S L,Ma M H,Xu S Y,et al.Research progress ofthallium pollution control technology in water[J].Water Treatment Technology,2014,40(2):15-19
• [2]邓红梅,陈永亨,刘涛,等.铊在土壤-植物系统中的迁移积累[J].环境化学,2013,32(9):1749-1757Deng H M,Chen Y H,Liu T,et al.Study on the translocation and accumulation of thallium in soil-plant system[J].Environmental Chemistry,2013,32(9):1749-1757
• [3]Babic'M,Radic'S,Cvjetko P,et.al.Antioxidative response of Lemna minor plants exposed to thallium(I)-acetate[J].Aquatic Botany,2009,(91):166-172
• [4]Ostga B K,Sadowska M,Ostrowska S.Thallium speciation in plant tissues-Tl(III)found in Sinapis alba L.grownin soil polluted with tailing sediment containing thalliumminerals[J].Talanta,2012,(93):326-329
• [5]Peter,A L J,Viraraghavan T.Thallium:A review ofpublic health and environmental concerns[J].Environment International,2005(31),493-501
• [6]Mestek O,Polák J,Jǔrǐcek M,et al.Trace elementdistribution and species fractionation in Brassica napusplant[J].Applied Organometallic Chemistry,2007,(21):468-474
• [7]邓红梅,陈永亨.水中铊的污染及其生态效应[J].环境化学,2008,27(3):363-367Deng H M,Chen Y H.Thallium pollution and its ecological effect in water[J].Environmental Chemistry,2008,27(3):363-367(in Chinese)
• [8]Lan C H,Lin T S.Acute toxicity of trivalent thalliumcompounds to Daphnia magna[J].Ecotoxicology andEnvironmental Safety,2005,(61):432-435
• [9]Ralph L,Twiss M R.Compatative toxicity of thallium(I),thallium(III),and cadmium(II)to the unicellular algaChlorella isolated from lake Erie[J].Environmental Contamination and Toxicology,2002,(68):261-268
• [10]Zitko V,Carson W V,Carson W G,et al.Thallium occurrence in the environment and toxicity to fish[J].Bulletin of Environmental Contamination and Toxicity,1975,13:23--30
• [11]Rybova R L,Nepurkova R,Metlicka J K,et al.Thallous ion interactions with transport of ions in the Algaehydrod ictyon reticulatum[J].Archiv fur HydrobiologieSupplement,1988,78:329-339
• [12]OECD.Test No.221:Lemna sp.Growth InhibitionTest[M].OECD Publishing,2006
• [13]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2006:164-169Li H S.Physiological and Biochemical Test Principle andTechnology of Plant[M].Beijing:Higher EducationPress,2006:164-169(in Chinese)
• [14]李仕飞,周建平.分光光度法测定植物过氧化氢酶活性的研究[J].安徽农学通报,2007,13(2):72-73Li S F,Zhou J P.Measuration of catalase vigor in plantswith spectrophotometry[J].Anhui Agricultural ScienceBulletin,2007,13(2):72-73(in Chinese)
• [15]周红卫,施国新,徐勤松.Cd2+污染水质对水花生根系抗氧化酶活性和超微结构的影响[J].植物生理学通讯,2003,39(3):211-214Zhou H W,Shi G X,Xu Q S.Effects of Cd2+pollutedwater on the activities of antioxidant enzymes and ultrastructure in roots of Alternanthera philoxeroides[J].Plant Physiology Communications,2003,39(3):211-214(in Chinese)
• [16]李君,周守标,黄文江,等.马蹄金叶片中铜、铅含量及其对生理指标的影响[J].应用生态学报,2004,15(12):2355-2358[[[Li J,Zhou S B,Huang W J,et al.Cu and Pb contents inDichondra repens leaf and their effects on its physiological indexes[J].Chinese Journal of Applied Ecology,2004,15(12):2355-2358(in Chinese)
• [17]Hou W,Chen X,Song G,et al.Effect of copper andcadmium on heavy metal polluted waterbody restorationby duckweed(Lemna minor)[J].Plant Physiology andBiochemistry,2007,45:62-69
• [18]徐楠,施国新,曾晓敏,等.Hg2+胁迫对浮萍体细胞DNA一级结构和抗氧化酶系的损伤[J].植物生态学报,2003,27(3):299-303Xu N,Shi G X,Zeng X M,et al.Damage to DNA primary structure and antioxidant enzymes in Lemna minorinduced by Hg2+[J].Acta Phytoecologica Sinica,2003,27(3):299-303
• [19]Asada K.The water-water cycle in chloroplasts:Scavenging of active oxygens and dissipation of excess photons[J].Annual Review of Plant Physiology and Plant Molecular Biology,1999,50:601-639