韩璐;尚小娜;于华;郭邑;么建萍;王雨新;刘晓晖;李亚晨;邵静;

• 1:大连市妇产医院暨妇幼保健院
• 2:大连医科大学公共卫生学院

摘要(Abstract)：

多溴联苯醚(polybrominated diphenyl ethers,PBDEs)是一类常用的溴代阻燃剂,由于使用广泛,已成为普遍存在的环境污染物。研究表明,PBDEs具有内分泌干扰作用,影响甲状腺功能,对胚胎发育、婴幼儿及儿童的健康存在潜在的危害。本研究采集了100例具羊水穿刺指征的孕妇的孕中期羊水样本,采用罗氏全自动电化学发光免疫分析仪测定羊水中促甲状腺激素(TSH)、三碘甲状腺原氨酸(TT3)、游离三碘甲状腺原氨酸(FT3)、甲状腺素(TT4)、游离甲状腺素(FT4)、抗甲状腺过氧化酶抗体(TPOA)和抗甲状腺球蛋白抗体(TGA)的含量,用气相色谱-负化学源-质谱法测定羊水样品中16种PBDEs同系物含量。结果显示,羊水中TSH、TT3、FT3、TT4、FT4中位数分别为0.342μIU·m L-1、0.949 nmol·L-1、1.52 pmol·L-1、11.02 nmol·L-1和3.91pmol·L-1,95%参考区间值分别为0.174~0.872μIU·m L-1、0.835~1.01 nmol·L-1、1.26~2.72 pmol·L-1、9.42~20.68 nmol·L-1和2.16~6.35 pmol·L-1。具有唐氏筛查高风险和甲状腺激素水平低下的羊水样品中共检出6种BDEs(BDE-47、BDE-71、BDE-138、BDE-183、BDE-190、BDE-209),其中BDE-71、183和190在被检羊水样品中均被检出,并且,TSH水平低下的羊水中BDE-190和209负荷水平显著高于正常组。这些结果提示,大连地区孕中期妇女羊水存在PBEDs内暴露,并且PBDEs内暴露可能与羊水的甲状腺激素水平低下、尤其TSH水平升高有关,提示PBDEs暴露可能与胎儿中枢神经系统损伤产生的下丘脑-垂体-甲状腺(HPT)轴功能失调有关。因此,羊水PBDEs内暴露对新生儿出生结局的影响值得进一步关注。

关键词(KeyWords)： PBDEs;甲状腺激素;羊水;唐氏筛查;气相色谱-负化学源-质谱检测

Abstract:

Keywords:

基金项目(Foundation): 辽宁省自然科学基金(2015020557);; 国家自然科学基金(81273031);; 国家科技部十二五支撑项目(No:2012BAI37B00;Sub-No:2012BAI37B03)

作者(Author): 韩璐;尚小娜;于华;郭邑;么建萍;王雨新;刘晓晖;李亚晨;邵静;

Email:

DOI:

参考文献(References)：

• [1]Siddiqi M A,Laessig R H,Reed K D.Polybrominated diphenyl ethers(PBDEs):New pollutants-old diseases[J].Journal of Clinical Medicine Research,2003,1(4):281-290
• [2]Darnerud P O,Eriksen G S,Jóhannesson T,et al.Polybrominated diphenyl ethers:Occurrence,dietary exposure,and toxicology[J].Environmental Health Perspectives,2001,109(Suppl1):49-68
• [3]Chen C,Chen J,Zhao H,et al.Levels and patterns of polybrominated diphenyl ethers in children's plasma from Dalian,China[J].Environment International,2010,36(2):163-167
• [4]李敬光,赵云峰,吴永宁.我国持久性有机污染物人体负荷研究进展[J].环境化学,2011,30(1):5-19Li J G,Zhao Y F,Wu Y N.A review for the body buden of persistent organic pollutants in China[J].Environmental Chemistry,2011,30(1):5-19(in Chinese)
• [5]万斌,郭良宏.多溴联苯醚的环境毒理学研究进展[J].环境化学,2011,30(1):143-152Wan B,Guo L H.Research progress on the investigation of environmental toxicology of polybrominated diphenyl ethers[J].Environmental Chemistry,2011,30(1):143-152(in Chinese)
• [6]Bernal J.Thyroid hormones and brain development[J].Vitamins&Hormones,2005,71:95-122
• [7]Glinoer D.Clinical and biological consequences of iodine deficiency during pregnancy[J].Endocrine Development,2007,10:62-85
• [8]Haddow J E,Palomaki G E,Allan W C,et al.Maternal thyroid deficiency during pregnancy and subsequent neuropsychological development of the child[J].The New England Journal of Medicine,1999,341(8):549-555
• [9]Zoeller R T,Crofton K M.Mode of action:Developmental thyroid hormone insufficiency--Neurological abnormalities resulting from exposure to propylthiouracil[J].Critical Reviews in Toxicology,2005,35(8-9):771-781
• [10]Thorpe-Beeston J G,Nicolaides K H,Mc Gregor A M.Fetal thyroid function[J].Thyroid,1992,2(3):207-217
• [11]Luton D,Huel C,Vuillard E,et al.Assessment of thyroid hormones and androgens in amniotic fluid:Clinical interest[J].Annales de Biologie Clinique,2009,67(3):299-305
• [12]朱立,赵志英,王正华,等.羊水中甲状腺激素检测及其临床应用[J].放射免疫学杂志,1999,12(1):4-6Zhu L,Zhao Z Y,Wang Z H,et al.Measurements of thyroid hormone levels in amniotic fluid and its clinical application[J].Journal of Radioimmunology,1999,12(1):4-6(in Chinese)
• [13]Singh P K,Parvin C A,Gronowski A M.Establishment of reference intervals for markers of fetal thyroid status in amniotic fluid[J].The Journal of Clinical Endocrinology&Metabolism,2003,88(9):4175-4179
• [14]钱波,王健,朱春艳,等.甲状腺激素脱碘酶在孕哺期十溴联苯醚(BDE 209)暴露致子代小鼠神经发育毒性中的潜在作用[J].生态毒理学报,2014,9(3):459-466Qian B,Wang J,Zhu C Y,et al.The role of thyroid hormone deiodinase in developmental neurotoxicity in newborn mice mediated by decabromodiphenyl ether(BDE209)exposure during pregnancy and lactation[J].Asian Journal of Ecotoxicology,2014,9(3):459-466(in Chinese)
• [15]Nderson G W,Schoonover C M,Jones S A.Control of thyroid hormone action in the developing rat brain[J].Thyroid,2003,13(11):1039-1056
• [16]Koopdonk-Kool J M,de Vijlder J J,Veenboer G J,et al.Type II and type III deiodinase activity in human placenta as a function of gestational age[J].The Journal of Clinical Endocrinology&Metabolism,1996,81(6):2154-2158
• [17]Rabeler R,Mittag J,Geffers L,et al.Generation of thyrotropin-releasing hormone receptor 1-deficient mice as an animal model of central hypothyroidism[J].Molecular Endocrinology,2004,18(6):1450-1460
• [18]Dingemans M M,van den Berg M,Westerink R H.Neurotoxicity of brominated flame retardants:(In)direct effects of parent and hydroxylated polybrominated diphenyl ethers on the(developing)nervous system[J].Environmental Health Perspectives,2011,119(7):900-907
• [19]刘早玲,张建清.多溴联苯醚对甲状腺激素干扰毒性的研究进展[J].环境与职业医学,2010,27(2):107-112Liu Z L,Zhang J Q.Progress in toxicity of polybrominated diphenyl ethers on the thyroid hormone disruption[J].Journal of Environmental&Occupational Medicine,2010,27(2):107-112(in Chinese)
• [20]颜世帅,徐海明,秦占芬.多溴二苯醚毒理学研究进展及展望[J].生态毒理学报,2010,5(5):609-617Yan S S,Xu H M,Qin Z F.Research progress and future perspectives in toxicology study on polybrominated diphenyl ethers[J].Asian Journal of Ecotoxicology,2010,5(5):609-617(in Chinese)
• [21]夏涛,何卫红,张明,等.2,2',4,4'-四溴联苯醚和2,2',4,4',5-五氯联苯联合作用的细胞遗传毒性[J].中华劳动卫生职业病杂志,2008,26(2):89-93Xia T,He W H,Zhang M,et al.Cyto-genotoxicity induced by 2,2',4,4'-tetrabromodiphenyl ethers combined with 2,2',4,4',5-hexachlorobiphenyl treatment in SH-SY5Y cells[J].Chinese Journal of Industrial Hygiene&Occupational Diseases,2008,26(2):89-93(in Chinese)
• [22]Gill U,Chu I,Ryan J J,et al.Polybrominated diphenyl ethers:Human tissue levels and toxicology[J].Reviews of Environmental Contamination and Toxicology,2004,183:55-97
• [23]Erlichman I,Mimouni F B,Erlichman M,et al.Thyroxine-based screening for congenital hypothyroidism in neonates with down syndrome[J].The Journal of Pediatrics,2016,173:165-168