赵宇侠;李晨红;黎文明;王志正;韩秋琴;陈培清;

• 1:上海健康医学院协同科研中心
• 2:国立药材研究院人参医药研究中心

摘要(Abstract)：

量子点(QDs)和Cu~(2+)可能共存于肝脏,对肝细胞产生联合毒性,威胁人体健康。本研究以人胚肝细胞(L02)为研究对象,考察了低/无毒的QDs(3.6%的细胞致死率)对Cu~(2+)诱导L02细胞毒性的影响及相关机理,为其可能的健康和环境风险提供参考。结果显示,QDs的加入使得Cu~(2+)的细胞毒性有了很大的提高,细胞存活率最高下降了26.0%,两者表现为协同作用;另外QDs存在使得Cu~(2+)细胞蓄积量增大,伴随着Cu~(2+)天然解毒蛋白CTR1和ATP7A等表达水平的升高,从侧面印证了Cu~(2+)的蓄积量增加对细胞的影响;同时,QDs的存在使得细胞乳酸脱氢酶(lactate dehyfrogenase,LDH)泄露相对于Cu~(2+)单独处理组提高,暗示QDs可能破坏了细胞膜,导致Cu~(2+)在细胞内蓄积量增加,从而使细胞毒性增加,两者的联合毒性表现为协同作用。

关键词(KeyWords)： 量子点纳米;Cu2+;L02细胞;联合毒性;细胞膜;蓄积量

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金“量子点与Cu2+对L02细胞联合毒性的木马机理研究”项目(21307031);; 上海健康医学院种子基金项目(E1-0200-19-201132);上海健康医学院百人库项目(B1-0200-19-311133)

作者(Author): 赵宇侠;李晨红;黎文明;王志正;韩秋琴;陈培清;

Email:

DOI:

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